LOCUS       sequence-332831-        8698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A codon-optimized Cas9 nuclease under the control of the Drosophila 
            hsp70 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings 
            AM, O'Connor-Giles KM
  TITLE     Highly specific and efficient CRISPR/Cas9-catalyzed 
            homology-directed repair in Drosophila.
  JOURNAL   Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. 
            Epub 2014 Jan 29.
  PUBMED    24478335
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.113.160713"; journalName: "Genetics"; date: 
            "2014-04"; volume: "196"; issue: "4"; pages: "961-71"
COMMENT     Sequence Label: pBS-Hsp70-Cas9
FEATURES             Location/Qualifiers
     source          1..8698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(36..52)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(36..52)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        76..528
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     475..496
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             547..612
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             619..639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             664..4764
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             664..4764
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4765..4812
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    4892..5764
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
     primer_bind     complement(5825..5844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5826..5844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5851..5868)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5851..5867)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5860..5882)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6009..6464
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6096..6115)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6306..6327
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6490..6594
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6595..6663,6664..7455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6813..6832)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7626..8214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8115..8134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8368..8385
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8502..8523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8538..8543,8544..8561,8562..8568)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8576..8592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8581..8603
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8600..8616
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8600..8616
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8637..8655
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     join(8683..8698,1)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     join(8684..8698,1..2)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta gagcggccgc
       61 ggaattccca tccccctaga atcccaaaac aaactggtta ttgtggtagg tcatttgttt
      121 ggcagaaaga aaactcgaga aatttctctg gccgttattc gttattctct cttttctttt
      181 tgggtctctc cctctctgca ctaatgctct ctcactctgt cacacagtaa acggcatact
      241 gctctcgttg gttcgagaga gcgcgcctcg aatgttcgcg aaaagagcgc cggagtataa
      301 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
      361 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa
      421 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
      481 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctggg ggatctgatc
      541 gatatggact ataaggacca cgacggagac tacaaggatc atgatattga ttacaaagac
      601 gatgacgata agatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca
      661 gccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg
      721 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
      781 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
      841 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
      901 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
      961 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
     1021 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
     1081 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
     1141 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     1201 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     1261 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     1321 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac
     1381 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     1441 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     1501 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     1561 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     1621 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     1681 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     1741 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     1801 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     1861 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     1921 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     1981 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     2041 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     2101 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     2161 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     2221 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     2281 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     2341 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     2401 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     2461 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     2521 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     2581 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     2641 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     2701 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     2761 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     2821 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     2881 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     2941 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     3001 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
     3061 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     3121 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat
     3181 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
     3241 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     3301 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
     3361 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     3421 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     3481 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
     3541 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     3601 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     3661 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     3721 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     3781 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     3841 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
     3901 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     3961 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
     4021 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
     4081 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     4141 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     4201 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     4261 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
     4321 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     4381 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     4441 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     4501 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
     4561 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
     4621 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
     4681 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     4741 gacctgtctc agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca
     4801 aaaaagaaaa agtaatctag agatcttcca tacctaccag ttctgcgcct gcagcaatgg
     4861 caacaacgtt gcccggatcg aggtcgacta aggccaaaga gtctaatttt tgttcatcaa
     4921 tgggttataa catatgggtt atattataag tttgttttaa gtttttgaga ctgataagaa
     4981 tgtttcgatc gaatattcca tagaacaaca atagtattac ctaattacca agtcttaatt
     5041 tagcaaaaat gttattgctt atagaaaaaa taaattattt atttgaaatt taaagtcaac
     5101 ttgtcattta atgtcttgta gacttttgaa agtcttacga tacaattagt atctaatata
     5161 catgggttca ttctacattc tatattagtg atgatttctt tagctagtaa tacattttaa
     5221 ttatattcgg ctttgatgat tttctgattt tttccgaacg gattttcgta gaccctttcg
     5281 atctcataat ggctcatttt attgcgatgg acggtcagga gagctccact tttgaatttc
     5341 tgttcgcaga caccgcattt gtagcacata gccgggacat ccggtttggg gagattttcc
     5401 agtctctgtt gcaattggtt ttcgggaatg cgttgcaggc gcatacgctc tatatcctcc
     5461 gaacggcgct ggttgaccct agcatttaca taaggatcag cagcaaaatt tgcctctact
     5521 tcattgcccg gaatcacagc aatcagatgt ccctttcggt tacgatggat attcaggtgc
     5581 gaaccgcaca caaagctctc gccgcacact ccacactgat atggtcgctc gcccgtgtgg
     5641 cgccgcatat ggatcttaag gtcgttggac tgcacaaagc tcttgctgca cattttgcag
     5701 gagtacggcc tttgacccgt gtgcaatcgc atgtgtcgcg ccagcttgtt ctgcgaaata
     5761 aactaacggg aattcctgca gcccggggga tccgcggccg ccaccgcggt ggagctccaa
     5821 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     5881 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     5941 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6001 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6061 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6121 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6181 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6241 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6301 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6361 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6421 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     6481 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6541 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6601 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6661 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6721 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6781 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6841 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6901 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6961 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7021 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7081 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7141 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7201 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7261 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7321 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7381 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7441 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7501 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7741 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8221 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8281 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8341 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8401 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8461 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     8521 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8581 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcgaaatt
     8641 aaccctcact aaagggaaca aaagctggta ccgggccccc cctcgaggtc gacggtat
//
