LOCUS       sequence-332833-        6398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5487:Dendra
FEATURES             Location/Qualifiers
     source          1..6398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2524..2547
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2804..3604
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3729..4317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4218..4237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatggcgtcg gctacgtgct gcgggaaacg cctccgtagc
      301 gagatggcct ttcctccgaa ctcctggcgg cccacgggcc cgctccccac cagctcactg
      361 tccttgcgct ggcgcgtgat gatgctcgcg atgtcgatgg tggcgctcgt ggtggtgctg
      421 atggccgtcg cggtgtacgc cgtcgtctcg cgcgcgctct acgacgacct cgacaaccag
      481 ctgcacagcc gggcccggct gctcatcgag agcgggtcgc tggccgccga tccgggcaag
      541 gccatcgagg gcaccgccta ctcggacgtc aacgcgatgc tggtgatccc gggccggtcg
      601 atctacaccg ccaaccagca gggtcagacg ctgccgctgg gagaaccgga gaaagacgtc
      661 atctccggcg aactgctcat gtcactgcgc acggccaatc atcagcgggt gctcgcggtg
      721 catctggcca acggcagttc actgctgatc tccaagagcc tcgcgccgac cgtccaagtg
      781 ctcagacggc tcggcacggt gctgctgatc gtcggcggta tcggcgtcgc cgtggccgcc
      841 atcgccggtg gggccgtcgc cagagcgggg ctgcgtcccg tcggcagact caccgaggcc
      901 gccgaacggg tggcccgcac cgacgaccta cgacccatcc cggtggtcgg cagcgacgag
      961 ctcgcgcgcc tcaccgaggc gttcaacatg atgttgcggg cgctggccga atcccgcgaa
     1021 cgccaggccc ggctggtctc cgacgcggga cacgaactgc gcacgccgct gacctcgctg
     1081 cgcaccaacg tcgaactgct gatggccgcg caggaacccg gtgcaccgcc gctgcccgag
     1141 gacgagatgg ccgggctgcg cgccgacgtg atcgcccaga tcgaggagtt gtccacgctc
     1201 gtgggcgatc tcgtggacct cacccgcgag gacgcgggcg gcatcacgcc cgagcccgtc
     1261 gacatggccg acgtcatcga ccgcagcctc gaacgggtcc gccgccgccg caacgacatc
     1321 gagttcgatg tcgacgtcat cggctggcag gtgttcggcg atgcacaggg tctgggccgc
     1381 gcggtgctca acctgctcga caacgccgcg aagtggagtc cgccgggcgg acgcgtgggc
     1441 gtccggctgc atcaggtcga ccacatgcat gccgaaatcg tcgtctccga ccagggaccc
     1501 ggcatcccgc cggaggagcg gcgcctggtg ttcgagcggt tctaccgctc cgacgcggcg
     1561 cgtgcgatgc ccggatccgg tctgggcctg gcgatcgtgc agcaggtggt cctgaaacac
     1621 ggtggcgcgc ttcgcatcga cgagacggtc cccggcggca acccgccggg cgcctcggtc
     1681 cacatgctgc tgcccggcca gcggatcccc gatccgggtg cgacacgctc cgccgagggg
     1741 ttcgtcgacg accggggcgg gcatactgtc gccaccgaaa agcttggtgc ggccgcgggc
     1801 aacacgcctg gcatcaatct gatcaaggag gacatgcgtg tcaaggtgca tatggagggc
     1861 aacgtgaacg gtcacgcctt cgtgatcgag ggggagggta agggtaagcc ttacgaaggg
     1921 acccagaccg ccaacctcac cgtcaaggag ggcgcccctc tcccattctc gtacgatatc
     1981 ctcactaccg cggtgcatta cgggaaccgc gtgttcacca agtaccccga ggatatccct
     2041 gactacttca agcagagctt cccggaggga tactcctggg agcggacgat gaccttcgaa
     2101 gacaagggta tctgtacgat ccgcagcgac atctcgctgg aaggggactg tttcttccag
     2161 aacgtccgtt tcaaggggac caactttccg ccgaacggcc cggtcatgca gaagaagacc
     2221 ctcaagtggg agccgagcac ggagaagctg cacgttcggg acggcctcct ggtcggcaac
     2281 atcaacatgg cgctgctgct ggaaggcggc ggccactacc tatgcgactt caagaccacg
     2341 tacaaagcga agaaggtcgt ccagctgccc gacgcgcact tcgtggacca ccgcatcgaa
     2401 atcctgggga acgattcgga ctacaacaag gtcaagttgt acgagcacgc tgtggcacgc
     2461 tatagcccgc tcccgtcgca ggtctggttc gaaggcggcg gcggggagaa cctgtatttc
     2521 caggactaca aggacgacga cgacaagtga tttaaataac cccttggggc ctctaaacgg
     2581 gtcttgaggg gttttttgtt aactagcgta cgatcgaatt cctgcagctc acggtaactg
     2641 atgccgtatt tgcagtacca gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg
     2701 cctttgaatg ggttcatgtg cagctccatc agcaaaaggg gatgataagt ttatcaccac
     2761 cgactatttg caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga
     2821 gtgcaatgtc gtgcaatacg aatggcgaaa agccgagctc atcggtcagc ttctcaacct
     2881 tggggttacc cccggcggtg tgctgctggt ccacagctcc ttccgtagcg tccggcccct
     2941 cgaagatggg ccacttggac tgatcgaggc cctgcgtgct acgctgggtc cgggagggac
     3001 gctcgtcatg ccctcgtggt caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc
     3061 cgttacaccg gaccttggag ttgtctctga cacattctgg cgcctgccaa atgtaaagcg
     3121 cagcgcccat ccatttgcct ttgcggcagc ggggccacag gcagagcaga tcatctctga
     3181 tccattgccc ctgccacctt actcgcctgc aagcccggtc gcccgtgtcc atgaactcga
     3241 tgggcaggta cttctcctcg gcgtgggaca cgatgccaac acgacgctgc atcttgccga
     3301 gttgatggca aaggttccct atggggtgcc gagacactgc accattcttc aggatggcaa
     3361 gttggtacgc gtcgattatc tcgagaatga ccactgctgt gagcgctttg ccttggcgga
     3421 caggtggctc aaggagaaga gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg
     3481 gttgatccgc tcccgcgaca ttgtggcgac agccctgggt caactgggcc gagatccgtt
     3541 gatcttcctg catccgccag aggcgggatg cgaagaatgc gatgccgctc gccagtcgat
     3601 tggctgagct catgagcgga gaacgagatg acgttggagg ggcaaggtcg cgctgattgc
     3661 tggggcaaca cgggggatcc actagttcca tgagcgtcag accccgtaga aaagatcaaa
     3721 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3781 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3841 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3901 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3961 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4021 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4081 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4141 cccgagggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4201 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4261 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4321 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4381 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4441 accgctcgcc gcagccgaac gaccgagcgc aacgcgtgcg gccgcggtac ccggggatcc
     4501 tctagagtcg accaccaagg gcaccatctc tgcttgggcc accccgttgg ccgcagccag
     4561 ctcgctgaga gccgtgaacg acagggcgaa cgccagcccg ccgacggcga gggttccgac
     4621 cgctgcaact cccggtgcaa ccttgtcccg gtctattctc ttcactgcac cagctccaat
     4681 ctggtgtgaa tgcccctcgt ctgttcgcgc aggcgggggg ctctattcgt ttgtcagcat
     4741 cgaaagtagc cagatcaggg atgcgttgca accgcgtatg cccaggtcag aagagtcgca
     4801 caagagttgc agacccctgg aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag
     4861 cggagcgggc gacgggaatc gaacccgcgt agctagtttg gaagaatggg tgtctgccga
     4921 ccacatatgg gccggtcaag ataggttttt accccctctc ggctgcatcc tctaagtgga
     4981 aagaaattgc aggtcgtaga agcgcgttga agcctgagag ttgcacagga gttgcaaccc
     5041 ggtagccttg ttcacgacga gaggagacct agttggcacg tcgcggatgg ggatcgctga
     5101 agactcagcg cagcgggagg atccaagcct catacgtcaa cccgcaggac ggtgtgaggt
     5161 actacgcgct gcagacctac gacaacaaga tggacgccga agcctggctc gcgggcgaga
     5221 agcggctcat cgagatggag acctggaccc ctccacagga ccgggcgaag aaggcagccg
     5281 ccagcgccat cacgctggag gagtacaccc ggaagtggct cgtggagcgc gacctcgcag
     5341 acggcaccag ggatctgtac agcgggcacg cggagcgccg catctacccg gtgctaggtg
     5401 aagtggcggt cacagagatg acgccagctc tggtgcgtgc gtggtgggcc gggatgggta
     5461 ggaagcaccc gactgcccgc cggcatgcct acaacgtcct ccgggcggtg atgaacacag
     5521 cggtcgagga caagctgatc gcagagaacc cgtgccggat cgagcagaag gcagccgatg
     5581 agcgcgacgt agaggcgctg acgcctgagg agctggacat cgtcgccgct gagatcttcg
     5641 agcactaccg gatcgcggca tacatcctgg cgtggacgag cctccggttc ggagagctga
     5701 tcgagcttcg ccgcaaggac atcgtggacg acggcatgac gatgaagctc cgggtgcgcc
     5761 gtggcgcttc ccgcgtgggg aacaagatcg tcgttggcaa cgccaagacc gtccggtcga
     5821 agcgtcctgt gacggttccg cctcacgtcg cggagatgat ccgagcgcac atgaaggacc
     5881 gtacgaagat gaacaagggc cccgaggcat tcctggtgac cacgacgcag ggcaaccggc
     5941 tgtcgaagtc cgcgttcacc aagtcgctga agcgtggcta cgccaagatc ggtcggccgg
     6001 aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga
     6061 cgaccaagga gctgatggcc cgtctcggtc acacgactcc taggatggcg atgaagtacc
     6121 agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc gatgtccaag ctggccaaga
     6181 cctcctgaaa cgcaaaaagc ccccctccca aggacactga gtcctaaaga ggggggtttc
     6241 ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga
     6301 gacctgggca ccagccccgc cgccgccagg agcattgccg ttcccgccag aaatctagac
     6361 ggtgaccaca acgcgcccgc tttgatcggg gacgtcga
//
