LOCUS       sequence-332838-        6275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5061:Dendra
FEATURES             Location/Qualifiers
     source          1..6275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2401..2424
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2681..3481
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3606..4194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4095..4114
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatgcgcgct cggcggctat gtgctgcggc agtggccgcg
      301 atggcggcgg catcgatggt gtcggcatgc ggttcacaga ccggcggcat cgtcatcaac
      361 tactacaccc cggcaaacga ggaagcgacg ttcaaagccg tcgccaaccg ctgcaacgaa
      421 caactcggcg gccggttcca gatcgcacag cggaatttgc ccaagggcgc cgacgaccaa
      481 cggttgcagt tggcgcgcag gctcaccggc aacgacaaga gcctcgacgt gatggcgctc
      541 gacgtggtgt ggaccgccga gttcgccgaa gccggttggg ccgtgccgct gtcggaggac
      601 cccgctggtc tcgccgaggc cgacgcgacg gagaacacgc tgcccgggcc actggagacg
      661 gcccgctggc aggatgagct ctacgccgcg cccatcacca ccaacaccca attgctgtgg
      721 taccgcgctg atctgatgcc cgcgccgccc accacgtggg acggcatgct cgacgaggcc
      781 aaccgcctgt accgcgaggg cggcccgagc tggattgcgg ttcagggcaa gcagtacgaa
      841 ggcatggtgg tgtggttcaa cacgctgctg cagagcgcgg gcggtcaggt gctgtccgac
      901 gacgggcagc gcgtcacgct caccgacacc cccgagcacc gtgcggcaac cgtcaaggcg
      961 ctgcggatca tcaaatcggt tgccaccgcg ccgggtgcgg acccctcgat cacccagacc
     1021 gacgagaaca ccgcccggct ggcactcgaa cagggcaagg ccgcgctcga ggtgaactgg
     1081 ccgtacgtgc ttccgtcgct gctggagaac gccgtcaagg gcggcgtgtc gttcctgccg
     1141 ctcgacggcg atcccgcgct gcagggcagc atcaacgacg tcggcacctt ctcgcccacc
     1201 gacgagcagt tcgacatcgc gttcgacgcg agcaagaagg tcttcgggtt cgcgccgtat
     1261 ccgggtgtga atcccgacga accggcccgg gtgaccctgg gcgggctgaa cctggccgtc
     1321 gcgagcacca gccagcacaa ggccgaggcg ttcgaggcga tacggtgcct gcgcaatgtg
     1381 gagaaccagc gctacacctc gatcgagggc ggtctgcccg ctgtgcgcac atcgctgtac
     1441 gacgatccgg cgttccagaa gaagtacccg cagtacgaga tcatccgtca gcagctcacc
     1501 aatgccgctg tgcggccggc cacgccggtc taccaggcgg tgtccacccg gatgtcggcc
     1561 acgctcgcac cgatctcgga catcgacccc gagcgcaccg cagacgaact caccgaagcc
     1621 gtacaaaagg cgatcgacgg taaggggctg attccgaagc ttggtgcggc cgcgggcaac
     1681 acgcctggca tcaatctgat caaggaggac atgcgtgtca aggtgcatat ggagggcaac
     1741 gtgaacggtc acgccttcgt gatcgagggg gagggtaagg gtaagcctta cgaagggacc
     1801 cagaccgcca acctcaccgt caaggagggc gcccctctcc cattctcgta cgatatcctc
     1861 actaccgcgg tgcattacgg gaaccgcgtg ttcaccaagt accccgagga tatccctgac
     1921 tacttcaagc agagcttccc ggagggatac tcctgggagc ggacgatgac cttcgaagac
     1981 aagggtatct gtacgatccg cagcgacatc tcgctggaag gggactgttt cttccagaac
     2041 gtccgtttca aggggaccaa ctttccgccg aacggcccgg tcatgcagaa gaagaccctc
     2101 aagtgggagc cgagcacgga gaagctgcac gttcgggacg gcctcctggt cggcaacatc
     2161 aacatggcgc tgctgctgga aggcggcggc cactacctat gcgacttcaa gaccacgtac
     2221 aaagcgaaga aggtcgtcca gctgcccgac gcgcacttcg tggaccaccg catcgaaatc
     2281 ctggggaacg attcggacta caacaaggtc aagttgtacg agcacgctgt ggcacgctat
     2341 agcccgctcc cgtcgcaggt ctggttcgaa ggcggcggcg gggagaacct gtatttccag
     2401 gactacaagg acgacgacga caagtgattt aaataacccc ttggggcctc taaacgggtc
     2461 ttgaggggtt ttttgttaac tagcgtacga tcgaattcct gcagctcacg gtaactgatg
     2521 ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa atgccggcct
     2581 ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta tcaccaccga
     2641 ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag cggtggagtg
     2701 caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc tcaaccttgg
     2761 ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc ggcccctcga
     2821 agatgggcca cttggactga tcgaggccct gcgtgctacg ctgggtccgg gagggacgct
     2881 cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca cgtcgcccgt
     2941 tacaccggac cttggagttg tctctgacac attctggcgc ctgccaaatg taaagcgcag
     3001 cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca tctctgatcc
     3061 attgcccctg ccaccttact cgcctgcaag cccggtcgcc cgtgtccatg aactcgatgg
     3121 gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc ttgccgagtt
     3181 gatggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg atggcaagtt
     3241 ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct tggcggacag
     3301 gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct ttgctcggtt
     3361 gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag atccgttgat
     3421 cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc agtcgattgg
     3481 ctgagctcat gagcggagaa cgagatgacg ttggaggggc aaggtcgcgc tgattgctgg
     3541 ggcaacacgg gggatccact agttccatga gcgtcagacc ccgtagaaaa gatcaaagga
     3601 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3661 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3721 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3781 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3841 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3901 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3961 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4021 gaggggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4081 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4141 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4201 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4261 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4321 gctcgccgca gccgaacgac cgagcgcaac gcgtgcggcc gcggtacccg gggatcctct
     4381 agagtcgacc accaagggca ccatctctgc ttgggccacc ccgttggccg cagccagctc
     4441 gctgagagcc gtgaacgaca gggcgaacgc cagcccgccg acggcgaggg ttccgaccgc
     4501 tgcaactccc ggtgcaacct tgtcccggtc tattctcttc actgcaccag ctccaatctg
     4561 gtgtgaatgc ccctcgtctg ttcgcgcagg cggggggctc tattcgtttg tcagcatcga
     4621 aagtagccag atcagggatg cgttgcaacc gcgtatgccc aggtcagaag agtcgcacaa
     4681 gagttgcaga cccctggaaa gaaaaatggc cagagggcga aaacaccctc tgaccagcgg
     4741 agcgggcgac gggaatcgaa cccgcgtagc tagtttggaa gaatgggtgt ctgccgacca
     4801 catatgggcc ggtcaagata ggtttttacc ccctctcggc tgcatcctct aagtggaaag
     4861 aaattgcagg tcgtagaagc gcgttgaagc ctgagagttg cacaggagtt gcaacccggt
     4921 agccttgttc acgacgagag gagacctagt tggcacgtcg cggatgggga tcgctgaaga
     4981 ctcagcgcag cgggaggatc caagcctcat acgtcaaccc gcaggacggt gtgaggtact
     5041 acgcgctgca gacctacgac aacaagatgg acgccgaagc ctggctcgcg ggcgagaagc
     5101 ggctcatcga gatggagacc tggacccctc cacaggaccg ggcgaagaag gcagccgcca
     5161 gcgccatcac gctggaggag tacacccgga agtggctcgt ggagcgcgac ctcgcagacg
     5221 gcaccaggga tctgtacagc gggcacgcgg agcgccgcat ctacccggtg ctaggtgaag
     5281 tggcggtcac agagatgacg ccagctctgg tgcgtgcgtg gtgggccggg atgggtagga
     5341 agcacccgac tgcccgccgg catgcctaca acgtcctccg ggcggtgatg aacacagcgg
     5401 tcgaggacaa gctgatcgca gagaacccgt gccggatcga gcagaaggca gccgatgagc
     5461 gcgacgtaga ggcgctgacg cctgaggagc tggacatcgt cgccgctgag atcttcgagc
     5521 actaccggat cgcggcatac atcctggcgt ggacgagcct ccggttcgga gagctgatcg
     5581 agcttcgccg caaggacatc gtggacgacg gcatgacgat gaagctccgg gtgcgccgtg
     5641 gcgcttcccg cgtggggaac aagatcgtcg ttggcaacgc caagaccgtc cggtcgaagc
     5701 gtcctgtgac ggttccgcct cacgtcgcgg agatgatccg agcgcacatg aaggaccgta
     5761 cgaagatgaa caagggcccc gaggcattcc tggtgaccac gacgcagggc aaccggctgt
     5821 cgaagtccgc gttcaccaag tcgctgaagc gtggctacgc caagatcggt cggccggaac
     5881 tccgcatcca cgacctccgc gctgtcggcg ctacgttcgc cgctcaggca ggtgcgacga
     5941 ccaaggagct gatggcccgt ctcggtcaca cgactcctag gatggcgatg aagtaccaga
     6001 tggcgtctga ggcccgcgac gaggctatcg ctgaggcgat gtccaagctg gccaagacct
     6061 cctgaaacgc aaaaagcccc cctcccaagg acactgagtc ctaaagaggg gggtttcttg
     6121 tcagtacgcg aagaaccacg cctggccgcg agcgccagca ccgccgctct gtgcggagac
     6181 ctgggcacca gccccgccgc cgccaggagc attgccgttc ccgccagaaa tctagacggt
     6241 gaccacaacg cgcccgcttt gatcggggac gtcga
//
