LOCUS       sequence-332842-        5771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5438:Dendra
FEATURES             Location/Qualifiers
     source          1..5771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1897..1920
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2177..2977
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3102..3690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3591..3610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatggcgaaa gagatcgact ttcgcccacg aaaggcattc
      301 ggacagaact tcgtccacga tgccaacacg gtgcggcgca tcgtgtcggc ctctggagtc
      361 catcggcacg accatgtcct cgaggtcggg ccaggactcg gttctctgac tctggcgctg
      421 ctggatcggg gagcccgcgt caccgcggtg gagatcgatc cactgctggc gcgtcagctg
      481 ccgacgacga tcgccgacca ttcgcacagt gagatcaacc ggctcacggt gctcaaccgg
      541 gacatcctga cgctcatgcc gggcgacctg gaggagcagc ccacggcact ggtggcgaac
      601 ctgccgtaca acgtggcggt gcccgcgttg ctgcacctgc tcgcggagtt cccgtcgatc
      661 cggacggtga tggtcatggt gcaggccgag gtggccgagc gcctggctgc cgaaccgggc
      721 ggcaaggact acggcgtgcc gagtgccaag gtgcggttct acggcaacgt gcgcaggcac
      781 ggcatggtct cgccgacggt cttctggccc atcccgcgcg tgtactcggg gctggtccgg
      841 atcgaccggt acgagacgtc gccgtggccc atggacgagg ccttccgcga cgaggtgttc
      901 aacctcatcg acatcggctt cgcgcagcgc cgcaagacct cgcgcaacgc gttcgccgag
      961 tgggccggtg gtggcaacga gtcggccgaa cgcctcctgg ctgccagaat cgacccggca
     1021 cgtcgcggtg agacgttgag catcaacgac ttcgtccggt tgctgcagcg gaccaaggaa
     1081 gcactgggcg aggactacac caagcaggtc gcaaggcctt ccgcatcagc gccttcggtg
     1141 tcagccgagg ccaagcttgg tgcggccgcg ggcaacacgc ctggcatcaa tctgatcaag
     1201 gaggacatgc gtgtcaaggt gcatatggag ggcaacgtga acggtcacgc cttcgtgatc
     1261 gagggggagg gtaagggtaa gccttacgaa gggacccaga ccgccaacct caccgtcaag
     1321 gagggcgccc ctctcccatt ctcgtacgat atcctcacta ccgcggtgca ttacgggaac
     1381 cgcgtgttca ccaagtaccc cgaggatatc cctgactact tcaagcagag cttcccggag
     1441 ggatactcct gggagcggac gatgaccttc gaagacaagg gtatctgtac gatccgcagc
     1501 gacatctcgc tggaagggga ctgtttcttc cagaacgtcc gtttcaaggg gaccaacttt
     1561 ccgccgaacg gcccggtcat gcagaagaag accctcaagt gggagccgag cacggagaag
     1621 ctgcacgttc gggacggcct cctggtcggc aacatcaaca tggcgctgct gctggaaggc
     1681 ggcggccact acctatgcga cttcaagacc acgtacaaag cgaagaaggt cgtccagctg
     1741 cccgacgcgc acttcgtgga ccaccgcatc gaaatcctgg ggaacgattc ggactacaac
     1801 aaggtcaagt tgtacgagca cgctgtggca cgctatagcc cgctcccgtc gcaggtctgg
     1861 ttcgaaggcg gcggcgggga gaacctgtat ttccaggact acaaggacga cgacgacaag
     1921 tgatttaaat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gttaactagc
     1981 gtacgatcga attcctgcag ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg
     2041 gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga atgggttcat gtgcagctcc
     2101 atcagcaaaa ggggatgata agtttatcac caccgactat ttgcaacagt gccgttgatc
     2161 gtgctatgat cgactgatgt catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg
     2221 aaaagccgag ctcatcggtc agcttctcaa ccttggggtt acccccggcg gtgtgctgct
     2281 ggtccacagc tccttccgta gcgtccggcc cctcgaagat gggccacttg gactgatcga
     2341 ggccctgcgt gctacgctgg gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct
     2401 ggacgacgag ccgttcgatc ctgccacgtc gcccgttaca ccggaccttg gagttgtctc
     2461 tgacacattc tggcgcctgc caaatgtaaa gcgcagcgcc catccatttg cctttgcggc
     2521 agcggggcca caggcagagc agatcatctc tgatccattg cccctgccac cttactcgcc
     2581 tgcaagcccg gtcgcccgtg tccatgaact cgatgggcag gtacttctcc tcggcgtggg
     2641 acacgatgcc aacacgacgc tgcatcttgc cgagttgatg gcaaaggttc cctatggggt
     2701 gccgagacac tgcaccattc ttcaggatgg caagttggta cgcgtcgatt atctcgagaa
     2761 tgaccactgc tgtgagcgct ttgccttggc ggacaggtgg ctcaaggaga agagccttca
     2821 gaaggaaggt ccagtcggtc atgcctttgc tcggttgatc cgctcccgcg acattgtggc
     2881 gacagccctg ggtcaactgg gccgagatcc gttgatcttc ctgcatccgc cagaggcggg
     2941 atgcgaagaa tgcgatgccg ctcgccagtc gattggctga gctcatgagc ggagaacgag
     3001 atgacgttgg aggggcaagg tcgcgctgat tgctggggca acacggggga tccactagtt
     3061 ccatgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3121 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3181 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3241 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3301 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3361 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3421 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3481 ctacagcgtg agctatgaga aagcgccacg cttcccgagg ggagaaaggc ggacaggtat
     3541 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3601 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3661 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3721 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3781 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3841 cgcaacgcgt gcggccgcgg tacccgggga tcctctagag tcgaccacca agggcaccat
     3901 ctctgcttgg gccaccccgt tggccgcagc cagctcgctg agagccgtga acgacagggc
     3961 gaacgccagc ccgccgacgg cgagggttcc gaccgctgca actcccggtg caaccttgtc
     4021 ccggtctatt ctcttcactg caccagctcc aatctggtgt gaatgcccct cgtctgttcg
     4081 cgcaggcggg gggctctatt cgtttgtcag catcgaaagt agccagatca gggatgcgtt
     4141 gcaaccgcgt atgcccaggt cagaagagtc gcacaagagt tgcagacccc tggaaagaaa
     4201 aatggccaga gggcgaaaac accctctgac cagcggagcg ggcgacggga atcgaacccg
     4261 cgtagctagt ttggaagaat gggtgtctgc cgaccacata tgggccggtc aagataggtt
     4321 tttaccccct ctcggctgca tcctctaagt ggaaagaaat tgcaggtcgt agaagcgcgt
     4381 tgaagcctga gagttgcaca ggagttgcaa cccggtagcc ttgttcacga cgagaggaga
     4441 cctagttggc acgtcgcgga tggggatcgc tgaagactca gcgcagcggg aggatccaag
     4501 cctcatacgt caacccgcag gacggtgtga ggtactacgc gctgcagacc tacgacaaca
     4561 agatggacgc cgaagcctgg ctcgcgggcg agaagcggct catcgagatg gagacctgga
     4621 cccctccaca ggaccgggcg aagaaggcag ccgccagcgc catcacgctg gaggagtaca
     4681 cccggaagtg gctcgtggag cgcgacctcg cagacggcac cagggatctg tacagcgggc
     4741 acgcggagcg ccgcatctac ccggtgctag gtgaagtggc ggtcacagag atgacgccag
     4801 ctctggtgcg tgcgtggtgg gccgggatgg gtaggaagca cccgactgcc cgccggcatg
     4861 cctacaacgt cctccgggcg gtgatgaaca cagcggtcga ggacaagctg atcgcagaga
     4921 acccgtgccg gatcgagcag aaggcagccg atgagcgcga cgtagaggcg ctgacgcctg
     4981 aggagctgga catcgtcgcc gctgagatct tcgagcacta ccggatcgcg gcatacatcc
     5041 tggcgtggac gagcctccgg ttcggagagc tgatcgagct tcgccgcaag gacatcgtgg
     5101 acgacggcat gacgatgaag ctccgggtgc gccgtggcgc ttcccgcgtg gggaacaaga
     5161 tcgtcgttgg caacgccaag accgtccggt cgaagcgtcc tgtgacggtt ccgcctcacg
     5221 tcgcggagat gatccgagcg cacatgaagg accgtacgaa gatgaacaag ggccccgagg
     5281 cattcctggt gaccacgacg cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc
     5341 tgaagcgtgg ctacgccaag atcggtcggc cggaactccg catccacgac ctccgcgctg
     5401 tcggcgctac gttcgccgct caggcaggtg cgacgaccaa ggagctgatg gcccgtctcg
     5461 gtcacacgac tcctaggatg gcgatgaagt accagatggc gtctgaggcc cgcgacgagg
     5521 ctatcgctga ggcgatgtcc aagctggcca agacctcctg aaacgcaaaa agcccccctc
     5581 ccaaggacac tgagtcctaa agaggggggt ttcttgtcag tacgcgaaga accacgcctg
     5641 gccgcgagcg ccagcaccgc cgctctgtgc ggagacctgg gcaccagccc cgccgccgcc
     5701 aggagcattg ccgttcccgc cagaaatcta gacggtgacc acaacgcgcc cgctttgatc
     5761 ggggacgtcg a
//
