LOCUS       sequence-332843-        6125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5470:Dendra
FEATURES             Location/Qualifiers
     source          1..6125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2251..2274
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2531..3331
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3456..4044
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3945..3964
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatgcgttcg gttgaggagc agcaggcccg tgtggcggcc
      301 gccgcggtgg cgccccgacc ggtacgggtg gccatagccg aatcgcaagg gctgatgtgt
      361 gccgaggagg tcgtcaccga acgacccatg ccgggattcg atcaggccgc catcgacggc
      421 tatgccgtgc gcagtgtcga cgtgctcggg gtcggttcgg attccgagga cggcggtgag
      481 atcagcctgc cggtgatggg cgtgatcgag gccggtgcgc gcacaccgag ccggctgcag
      541 ccgcggcagg ccgcacgggt tcagaccggc gcgccgatgc cgacgctggc cgacgcggtg
      601 ctcccgctgc gctggaccga cggcggcgag aaccgcgtgc gggtgttgcg cggcgtgcgc
      661 tctggcgcgt acgtgcggcg cacgggcgac gacgtgcaac ccggcgatgt cgcggtgcgt
      721 gcgggcacga tcatcggccc ggcccaggtg ggcctgctcg cggccgtcgg acgtgacaag
      781 gtgctggtgc atccgcggcc gcggctgtcg gtgatgtgcg tgggcggcga gctcgtcgac
      841 gtgtcgcgca ccccgggtac cgggcaggtg tacgacgtga actcctatgc actggccgcg
      901 gccggacgtg atgccggggc cgaggtgaac cgcgtcggca tcatcagcac cgatccccgt
      961 gaactgcgcg aaaccgttga gggacaggtc aaccgcaacg agatcgtggt gatcgcgggc
     1021 gccgtgggcg gggccgccgc ggaatcggtg cgcacggtgc tgtccgaact cggcgacatg
     1081 gaggtcgcgc gcatcgcgat gcacccgggc tcggtgcagg gcttcgggca gctgggccgc
     1141 gatcgcgtcc cggtgttcct gctgcctgcc aacccggtca gcgcgctcgt ggtgttcgag
     1201 atcatggtgc gcccgctgat ccggttgtcg ctgggcaagc gtcagccgat gcggcgcatc
     1261 gtgcaggcgc gcacgctgtc gccgatcacc tcgatgccgg gccgcaaggg gtatctgcgc
     1321 ggacaactca tgcgcgatca ggacaccggc gagtacctgg tgcaggcgct cggcggtgcg
     1381 cccggcgcct cgacccatct gctcgccact ctcgcggagg ccaactgtct ggtgatcgtg
     1441 cccgccgacg tcgaagaggt acgcacgggg gagacggtcg acgtcgcgtt cctggcgcag
     1501 cgcggcaagc ttggtgcggc cgcgggcaac acgcctggca tcaatctgat caaggaggac
     1561 atgcgtgtca aggtgcatat ggagggcaac gtgaacggtc acgccttcgt gatcgagggg
     1621 gagggtaagg gtaagcctta cgaagggacc cagaccgcca acctcaccgt caaggagggc
     1681 gcccctctcc cattctcgta cgatatcctc actaccgcgg tgcattacgg gaaccgcgtg
     1741 ttcaccaagt accccgagga tatccctgac tacttcaagc agagcttccc ggagggatac
     1801 tcctgggagc ggacgatgac cttcgaagac aagggtatct gtacgatccg cagcgacatc
     1861 tcgctggaag gggactgttt cttccagaac gtccgtttca aggggaccaa ctttccgccg
     1921 aacggcccgg tcatgcagaa gaagaccctc aagtgggagc cgagcacgga gaagctgcac
     1981 gttcgggacg gcctcctggt cggcaacatc aacatggcgc tgctgctgga aggcggcggc
     2041 cactacctat gcgacttcaa gaccacgtac aaagcgaaga aggtcgtcca gctgcccgac
     2101 gcgcacttcg tggaccaccg catcgaaatc ctggggaacg attcggacta caacaaggtc
     2161 aagttgtacg agcacgctgt ggcacgctat agcccgctcc cgtcgcaggt ctggttcgaa
     2221 ggcggcggcg gggagaacct gtatttccag gactacaagg acgacgacga caagtgattt
     2281 aaataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgttaac tagcgtacga
     2341 tcgaattcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg tacggcccac
     2401 agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag ctccatcagc
     2461 aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta
     2521 tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc
     2581 cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca
     2641 cagctccttc cgtagcgtcc ggcccctcga agatgggcca cttggactga tcgaggccct
     2701 gcgtgctacg ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag gtctggacga
     2761 cgagccgttc gatcctgcca cgtcgcccgt tacaccggac cttggagttg tctctgacac
     2821 attctggcgc ctgccaaatg taaagcgcag cgcccatcca tttgcctttg cggcagcggg
     2881 gccacaggca gagcagatca tctctgatcc attgcccctg ccaccttact cgcctgcaag
     2941 cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg tgggacacga
     3001 tgccaacacg acgctgcatc ttgccgagtt gatggcaaag gttccctatg gggtgccgag
     3061 acactgcacc attcttcagg atggcaagtt ggtacgcgtc gattatctcg agaatgacca
     3121 ctgctgtgag cgctttgcct tggcggacag gtggctcaag gagaagagcc ttcagaagga
     3181 aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg tggcgacagc
     3241 cctgggtcaa ctgggccgag atccgttgat cttcctgcat ccgccagagg cgggatgcga
     3301 agaatgcgat gccgctcgcc agtcgattgg ctgagctcat gagcggagaa cgagatgacg
     3361 ttggaggggc aaggtcgcgc tgattgctgg ggcaacacgg gggatccact agttccatga
     3421 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3481 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3541 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3601 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3661 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3721 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3781 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3841 cgtgagctat gagaaagcgc cacgcttccc gaggggagaa aggcggacag gtatccggta
     3901 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3961 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4021 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4081 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4141 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcaac
     4201 gcgtgcggcc gcggtacccg gggatcctct agagtcgacc accaagggca ccatctctgc
     4261 ttgggccacc ccgttggccg cagccagctc gctgagagcc gtgaacgaca gggcgaacgc
     4321 cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct tgtcccggtc
     4381 tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg ttcgcgcagg
     4441 cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg cgttgcaacc
     4501 gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa gaaaaatggc
     4561 cagagggcga aaacaccctc tgaccagcgg agcgggcgac gggaatcgaa cccgcgtagc
     4621 tagtttggaa gaatgggtgt ctgccgacca catatgggcc ggtcaagata ggtttttacc
     4681 ccctctcggc tgcatcctct aagtggaaag aaattgcagg tcgtagaagc gcgttgaagc
     4741 ctgagagttg cacaggagtt gcaacccggt agccttgttc acgacgagag gagacctagt
     4801 tggcacgtcg cggatgggga tcgctgaaga ctcagcgcag cgggaggatc caagcctcat
     4861 acgtcaaccc gcaggacggt gtgaggtact acgcgctgca gacctacgac aacaagatgg
     4921 acgccgaagc ctggctcgcg ggcgagaagc ggctcatcga gatggagacc tggacccctc
     4981 cacaggaccg ggcgaagaag gcagccgcca gcgccatcac gctggaggag tacacccgga
     5041 agtggctcgt ggagcgcgac ctcgcagacg gcaccaggga tctgtacagc gggcacgcgg
     5101 agcgccgcat ctacccggtg ctaggtgaag tggcggtcac agagatgacg ccagctctgg
     5161 tgcgtgcgtg gtgggccggg atgggtagga agcacccgac tgcccgccgg catgcctaca
     5221 acgtcctccg ggcggtgatg aacacagcgg tcgaggacaa gctgatcgca gagaacccgt
     5281 gccggatcga gcagaaggca gccgatgagc gcgacgtaga ggcgctgacg cctgaggagc
     5341 tggacatcgt cgccgctgag atcttcgagc actaccggat cgcggcatac atcctggcgt
     5401 ggacgagcct ccggttcgga gagctgatcg agcttcgccg caaggacatc gtggacgacg
     5461 gcatgacgat gaagctccgg gtgcgccgtg gcgcttcccg cgtggggaac aagatcgtcg
     5521 ttggcaacgc caagaccgtc cggtcgaagc gtcctgtgac ggttccgcct cacgtcgcgg
     5581 agatgatccg agcgcacatg aaggaccgta cgaagatgaa caagggcccc gaggcattcc
     5641 tggtgaccac gacgcagggc aaccggctgt cgaagtccgc gttcaccaag tcgctgaagc
     5701 gtggctacgc caagatcggt cggccggaac tccgcatcca cgacctccgc gctgtcggcg
     5761 ctacgttcgc cgctcaggca ggtgcgacga ccaaggagct gatggcccgt ctcggtcaca
     5821 cgactcctag gatggcgatg aagtaccaga tggcgtctga ggcccgcgac gaggctatcg
     5881 ctgaggcgat gtccaagctg gccaagacct cctgaaacgc aaaaagcccc cctcccaagg
     5941 acactgagtc ctaaagaggg gggtttcttg tcagtacgcg aagaaccacg cctggccgcg
     6001 agcgccagca ccgccgctct gtgcggagac ctgggcacca gccccgccgc cgccaggagc
     6061 attgccgttc ccgccagaaa tctagacggt gaccacaacg cgcccgcttt gatcggggac
     6121 gtcga
//
