LOCUS       sequence-33285-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter
            for optogenetic inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  TITLE     eNpHR3.0-EYFP
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-CaMKIIa-eNpHR 3.0-EYFP
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(73..783,784..786))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(115..136)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     464..483
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     723..744
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tttgtaatcc agaggttgat tatcgataag cttgatatcg aattctcatt acacctcgtt
       61 ctcgtagcag aacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      121 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta
      181 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      241 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
      301 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
      361 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      421 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      481 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      541 gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac tgcaggccgt agccgaaggt
      601 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
      661 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
      721 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
      781 gctcaccacg ttgatgtcga tctggtcca
//
