LOCUS       sequence-332866-       13176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-Host range expression vector, pBBR origin, LuxR activator, 
            PLuxB promoter, Violacein, Gentamicin Resistance Marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Schuster LA, Reisch CR
  TITLE     A plasmid toolbox for controlled gene expression across the 
            Proteobacteria.
  JOURNAL   Nucleic Acids Res. 2021 Jun 14. pii: 6298615. doi: 
            10.1093/nar/gkab496.
  PUBMED    34125913
REFERENCE   2  (bases 1 to 13176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jun 14. pii: 6298615. doi: 10.1093/nar/gkab496."
COMMENT     Sequence Label: pBLxVi5
FEATURES             Location/Qualifiers
     source          1..13176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1055..1826
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1827..2489
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3183..3205
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3197..3214
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3198..3214
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        4192..4241
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             4286..4297
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             7737..7745
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             11734..11751
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(11804..11822)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11818..11865
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        join(12199..12204,12205..12221,12222..12227)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             12416..12949
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gtccagaacg gtcttcaggc gctcccgaag gtctcgggcc gtctcttggg cttgatcggc
       61 cttcttgcgc atctcacgcg ctcctgcggc ggcctgtagg gcaggctcat acccctgccg
      121 aaccgctttt gtcagccggt cggccacggc ttccggcgtc tcaacgcgct ttgagattcc
      181 cagcttttcg gccaatccct gcggtgcata ggcgcgtggc tcgaccgctt gcgggctgat
      241 ggtgacgtgg cccactggtg gccgctccag ggcctcgtag aacgcctgaa tgcgcgtgtg
      301 acgtgccttg ctgccctcga tgccccgttg cagccctaga tcggccacag cggccgcaaa
      361 cgtggtctgg tcgcgggtca tctgcgcttt gttgccgatg aactccttgg ccgacagcct
      421 gccgtcctgc gtcagcggca ccacgaacgc ggtcatgtgc gggctggttt cgtcacggtg
      481 gatgctggcc gtcacgatgc gatccgcccc gtacttgtcc gccagccact tgtgcgcctt
      541 ctcgaagaac gccgcctgct gttcttggct ggccgacttc caccattccg ggctggccgt
      601 catgacgtac tcgaccgcca acacagcgtc cttgcgccgc ttctctggca gcaactcgcg
      661 cagtcggccc atcgcttcat cggtgctgct ggccgcccag tgctcgttct ctggcgtcct
      721 gctggcgtca gcgttgggcg tctcgcgctc gcggtaggcg tgcttgagac tggccgccac
      781 gttgcccatt ttcgccagct tcttgcatcg catgatcgcg tatgccgcca tgcctgcccc
      841 tcccttttgg tgtccaaccg gctcgacggg ggcagcgcaa ggcggtgcct ccggcgggcc
      901 actcaatgct tgagtatact cactagactt tgcttcgcaa agtagtgacc gcctacggcg
      961 gctgcggcgc cctacgggct tgctctcagg gcttcgccct gcgcggtcgc tgcgctccct
     1021 tgccagcccg tggatatgtg gacgatggcc gcgagcggcc accggctggc tcgcttcgct
     1081 cggcccgtgg acaaccctgc tggacaagct gatggacagg ctgcgcctgc ccacgagctt
     1141 gaccacaggg attgcccacc ggctacccag ccttcgacca catacccacc ggctccaact
     1201 gcgcggcctg cggccttgcc ccatcaattt ttttaatttt ctctggggaa aagcctccgg
     1261 cctgcggcct gcgcgcttcg cttgccggtt ggacaccaag tggaaggcgg gtcaaggctc
     1321 gcgcagcgac cgcgcagcgg cttggccttg acgcgcctgg aacgacccaa gcctatgcga
     1381 gtgggggcag tcgaagggcg aagcccgccc gcctgccccc cgagcctcac ggcggcgagt
     1441 gcgggggttc caagggggca gcgccacctt gggcaaggcc gaaggccgcg cagtcgatca
     1501 acaagccccg gaggggccac tttttgccgg agggggagcc gcgccgaagg cgtgggggaa
     1561 ccccgcaggg gtgcccttct ttgggcacca aagaactaga tatagggcga aatgcgaaag
     1621 acttaaaaat caacaactta aaaaaggggg gtacgcaaca gctcattgcg gcaccccccg
     1681 caatagctca ttgcgtaggt taaagaaaat ctgtaattga ctgccacttt tacgcaacgc
     1741 ataattgttg tcgcgctgcc gaaaagttgc agctgattgc gcatggtgcc gcaaccgtgc
     1801 ggcaccccta ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag
     1861 ccaagaacaa gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg
     1921 ggcttattgc gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg
     1981 gccaccagaa cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt
     2041 tgcggacggt ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga
     2101 agctcaacgg ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc
     2161 agccccgcga ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg
     2221 accaggacga atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg
     2281 gcgagcagca actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg
     2341 gcatggaacc agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc
     2401 agcgcctgcc gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc
     2461 cgacacgggt cacgctgccg cgccggtagc acttgggttg cgcagcaacc cgtaagtgcg
     2521 ctgttccaga ctatcggctg tagccgcctc gccgccctat accttgtctg cctccccgcg
     2581 ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg
     2641 ctaacggatt caccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa
     2701 ttattacctc cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg
     2761 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
     2821 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
     2881 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc
     2941 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac ggcctattgg ttaaaaaatg
     3001 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcca
     3061 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     3121 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     3181 ttcccagtca cgacgttgta aaacgacggc cagtgatcaa tttttaaagt atgggcaatc
     3241 aattgctcct gttaaaattg ctttagaaat actttggcag cggtttgttg tattgagttt
     3301 catttgcgca ttggttaaat ggaaagtgac agtacgctca ctgcaaccta atatttttga
     3361 aatatcccaa gagctttttc cttcgcatgc ccacgctaaa cattcttttt ctcttttggt
     3421 taaatcgttg tttgatttat tatttgctat atttattttt cgataattat caactagaga
     3481 aggaacaatt aatggtatgt tcatacacgc atgtaaaaat aaactatcta tatagttgtc
     3541 tttttctgaa tgtgcaaaac taagcattcc gaagccattg ttagccgtat gaatagggaa
     3601 actaaaccca gtgataagac ctgatgtttt cgcttcttta attacatttg gagatttttt
     3661 atttacagca ttgttttcaa atatattcca attaattggt gaatgattgg agttagaata
     3721 atctactata ggatcatatt ttatcaaatt agcgtcatca taatattgcc tccatttttt
     3781 agggtaatta tctaggattg aaatatcaga tttaaccata gaatgaggat aaatgatcgc
     3841 gagtaaataa tattcacaat gtaccatttt agtcatatca gataagcatt gattaatatc
     3901 attattgctt ctacaagctt taattttatt aattattctg tatgtgtcgt cggcatttat
     3961 gtttttcatt ctgcccctcc gaaaggctta agtcctcgcg tagtacctgg gcgctatcat
     4021 gccataccgc gaaaggtttt gcgccattcg atggcgcgcc gcccaattat tgaaggcctc
     4081 cctaacgggg ggcctttttt tgtttctggt ctcccgctta acgatcgttg gctgacctgt
     4141 aggatcgtac aggtttacgc aagaaaatgg tttgttacag tcgaataaaa gctgtcaccg
     4201 gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca gcctctacaa ataattttgt
     4261 ttaagaactg ggaagagcgc agattaaaga ggagaaatac tagatgacgg ttagagaaaa
     4321 caccatctca atcatcggag cgggcatttc aggtattttt tgcgcgctaa cgttagcaaa
     4381 atcaaaagag tgtaaaaata caaccattcg ggtttatgag cataaggatc gcgtgggtgg
     4441 tcgcgcacat gccgtaaaag tcaatgatca ctatgttgac ttaggtgctg gacgcttttc
     4501 ttcagggctt catcataatg tcaaagagca agttaagttg cttgatttgg catatgaaaa
     4561 attccctttt acacaactaa aaagaccgca agcattgcat aaaagtttaa agagcattct
     4621 gaaaaatttg aaaccacttt gtgatgaaca tccccacgat tcatttgaag agtttttaac
     4681 tatttacgta ggcagaatcc aagcaaaaga aatgatcaac gcattgggtt atgactcttt
     4741 agctctgccg aatattacgc cacatatcgc ttacgatata attgaaaaac accccgagat
     4801 tcaggggttt tctgacaatg ctggctatga gtggtttaac ctgaaggaag gcttttctgc
     4861 actgaccgag aagctctatg agcaggctct agcacacggt gttgagtttc attttggaca
     4921 tgagctgatc agtctagaag ctgatactgc gtccccttca ctcatattga aaaaagacaa
     4981 tcagcaaaca gtgatcatag acgaaggtga aatgatttta acttttcctc caacagctat
     5041 gaaaaagcta gattgtggtt ttccacaaga ttggagcaac tatacctatg gttcaattcc
     5101 gttgttcaaa ggcttcttgt tttacgatgc accttggtgg caagagctcg acttagccga
     5161 tcatgttgtc attgccaaca gcccgatgcg caagctgtat tttaaggaca atagatatgt
     5221 atttttttat accgatagtg aatatgcgaa cttttggcgt gacgccacat taaaaggtga
     5281 agcacattac ttacaaacgg taaaagaact tatggcagag gcgctaaagt gtcatgtaag
     5341 ccaactccca gatcccaaca atcacacata caaacactgg gtccatggtg ttgagttctc
     5401 ccaagaatct tgcccaaatc accctgaaac acttgaacac aaacggtctc atatcatctc
     5461 agcatcagat gcttatacat ctcattgtgg ttggatggag ggcggcatca tggcaggaac
     5521 gagtgctgca aaaacattgc ttacacgaat taagcaatct caagcaaagg ctgtaacaaa
     5581 gagtgagctt gtacacgttt aactgtcatt tgataaagac atactgtaaa caaagtaact
     5641 aggaagtttt aaataaatga gtattctcga ttttccccga gttcatttta aaggggttgc
     5701 gcgtgtcaat gtgccaacag gtaatcgtaa tattaacaat accttagata tatcgacaaa
     5761 cacggcgatc aaggatggta aaccctacga cgtagcaaag ccaccgcagg cctttcatga
     5821 ctacgtaaag tcattcgggc caaaatttaa tgcacacggc cgtcttgacg atgaaggtga
     5881 atttaaccta gctgctggtt acaacatgtg tggtaataac cacttcagct gggaaaacac
     5941 cttaattaca tcggttcaac taaaacctgg tgaatacatt accgaagata agttagtcgg
     6001 caataaactg tcactttggg ggcattacaa cgaatatttg agaacatcat ttaatcgtgc
     6061 acgctgggtc gacaacgacc cgacacgaag agattcggca ctcatttatg ctggtcaact
     6121 gactatcaca gaccctaaat cgtcagccaa tactgcacat attttatcag cagacattga
     6181 ctgcacacat ggtgttcgat gggttaaccg tggttatatt gaagagccga ttaaccactt
     6241 tctccatgaa gaaatgagcg aagctcgtct gtttcagttc acagtaaaaa aagacagtga
     6301 agaatttata tttaatcagc taggtcttga gtctgaattt ctatcagtat tgaaagataa
     6361 gctaaaagac ccaacggtga aaggcttagt ggtacagtac tgtgtaagta acctgtctcc
     6421 gcctatgaca cctaacaaac ccgttttttg cgacttacac ggctccattg gtttgtggta
     6481 cgacgatgac atgaaaacct ctccaacagg acgaatttta aggccggata atagtgagca
     6541 gtacgcgcct attgcgctat cagttaaaga taactggctc agtctaaaca gtgcgattag
     6601 ttttcctcat caaaattatg aacaagctat tccggttcaa aacggcatgc cacctgcact
     6661 aagtggcaag gttaacttgg gtgatctgta cttgaaatcc aatagtggtc aggtgttagc
     6721 agtgatacaa ccgcaagact acataaacaa tcacctcaat gctggtgtca ttgatattcc
     6781 cttgcctgat atcgatgcac aatttcatca gcaatcttta aagctatgct cagagcggca
     6841 cacttggtac gaaagtgatt ggtatattca agctgagcaa cacatcctct ctatggaaag
     6901 tccaaacaag gagctaaata agccaagtac acagtcactg gacatacgca gctactacag
     6961 aggtgagcct gcatctgtca gtcaattggc cacccacgtg ttaacaccta acacagtggc
     7021 ttgctctcct tttgttgaaa cagatgacaa tgggcgagga aaactcgaca ttgagagcct
     7081 tggtgcaggt agcgcagagc tctttttagg agagttttat agtccagtgc aaatccgtgt
     7141 gcttgcagat gattgggcat tggctgatac gcctacagag ctggttgact atgactttct
     7201 ctatacccat gttatggcct attacgaact tttttatcca tttatgactg aagcggtatt
     7261 cagtatggcg gataagtgta aatgtgaaac ctatgcccga ctaatgtggc aaatgtgtga
     7321 cccaaaaaac cgcgacaaaa gctattacat gccgagcact cgggagatgc cagaagtcca
     7381 ctcacagctg tttttaaagt atctgtgtaa tgtagaacaa tccgctatgc caaatatatt
     7441 gccagcagcg caatccgagg caaagatccg cggcaaaatc aaaaacaaaa agcagctgat
     7501 cagcgcacta aaagacgctg tggacctaga gctttcaatt atgctgcaat atatatacag
     7561 cgcctactca ttacctaact atgctattgg cgagcactta gtctcgcttg gacgctggaa
     7621 cgagcaacaa ctagaacttg taaatggtgg caaagacaga cgactcaata gcggctggcg
     7681 cggtgcatta cttgaaatcg cacacgaaga aatgatccac tatttagtgg taaacaacct
     7741 ccttatggca ttgggcgagc cgttttatcc aggcaagccg gttttgggag agcaagcaaa
     7801 agacactttt ggtctcgata ctgagttttc attcgaacca ttttcagagc acatagttgc
     7861 caaatttgtt cgctttgagt ggccagcgtt tttcccaacc gtcgggaagt ctattgcaga
     7921 cttttatgcc gatatcacaa aagcaatcga tgaactacct gacttatttg gtgaagagaa
     7981 gatcaatttg ggcggcgagc atcatttgtt tctcaatgaa attatcaatc gagaatatcc
     8041 aggctatcag tttgaagttt acgataagga gacagcctta ttcgctatta acttcgtgac
     8101 tgagcaaggc gaaggagttg ctgctgagtc cccccaattt gaagtcagtc attttagtcg
     8161 tttacgagcg atatcaaaag ccttaactaa cagtgatatc ccatttgaac cagcatatcc
     8221 agtgctgaaa aatcccgtat ttgaagcacg aagcgggtgt aacctcgtga cggaccccgc
     8281 agcagtagaa cttatgcagt tttataaagg gtgccatgaa ttaatgttcc agctgatgat
     8341 gcaacacttt gctatcaagc cgctgggcag tatgcgccgt tcacgattaa tgaatggtgc
     8401 tatcgatctc atgaccggga tattaaggcc actttcagta ctacttatga cattaccttc
     8461 aggcgtacca ggtagaaacg cgggtccacc tgtacctgaa gctattaaca cacacgtatt
     8521 tccagaccta gagcaagggt gtatggccat atctgagcaa tgtaaaaagc ttgcagctat
     8581 gggtagagct atggtctgtg caaaacctcc aacaacacaa attgagttat tagagttttt
     8641 tcaaaatcag atgcatgaaa tcgcaactgg caaactttca agggaagctt aatcatgaag
     8701 aaaataattg tcattggcgg cggtctagcc ggtggcctga cagccatata cttagcaaga
     8761 cgcggatacg atgtacacat catagagaaa cgtggaaacc ctctattgag ccaagcagac
     8821 tacattgatc aggtcagctc acgcgccatt ggtgtaagta tgactgtgcg gggcatagag
     8881 gctgtggttg cggcagggat cccgcttgaa gagcttcaag catgcggtgt agaagtatct
     8941 ggcatgtcgt tttttgttaa aggagagcca aaaacgcgtg aactaccccc gcttgataaa
     9001 ctcaagccgc tatctctcag tcgcagtgcg tttcaagttt tgttaaacaa atatgcggaa
     9061 cttgcaggtg tgaactatca ctatgatcaa cgctgtatcg aagtcaattt agataactgc
     9121 tcagtgatga caaaagacag caatgacact tttgttactc ataaaggtga tttacttatc
     9181 ggtgcagatg gcgcgcgttc gtgtgtcaga gaagcgatgc agtcccattg taggcgtttt
     9241 gagttccacc aatcattttt caaacatggt tataaaacac tggttgtacc cgatgcatct
     9301 aaagttggat tgcgcaaaga tctcctgtac ttctttggca tggactcagc aggacaattt
     9361 gcagggcgtg ctgcgacaat ccccgacggc agtatcagct ttgcggtttg ccttccttat
     9421 acgggtcaag tgagcttgca cagtgacgac agcaccgcaa tgcgagcatt cttcgacaaa
     9481 tactacccaa tggttcctga agatattcgt caagatatgt ttgagcaatt catgatcaag
     9541 cctagcaatg acttaatcaa cgtgcgctca tctacgttcc attataaaaa caccgcatta
     9601 ctgattggcg attcagccca tgcgaccgca ccatttttag gtcaagggat gaatatggcg
     9661 ctcgaagacg cctatgtatt ggacaccttg tttgaccaat atggcgatga ctttaccaag
     9721 atcctcgaag aatttaccgt tcagcgcaaa attgaagccg atgccatgca ggatatggcc
     9781 cgtgctaact atgaagttct tagttgctca gaccctatct tttttatgcg agctcgattt
     9841 gcacgctata tgaatcagaa atttccagat cattacccgc cagatatggc agaaaaactt
     9901 tacttcacat caatgcgtta cagtgaatta agagataagc aagaaaagca aaacgtttgg
     9961 tacaaaatag ggagaatgaa ttaatgaaga ttttagttgt tggtgccggt ccatcaggtc
    10021 tgatgtttgc tagtgaaacc aaaaagttaa agccagaatg ggaaattcgt atcattgaga
    10081 aaaaccaccc tgacgccaac gtcgttggct ggggtgttgt actgccaggg agagcgccgc
    10141 atcacccggc taaccctttg tcatatttag aaaaccatga aatactcgat gcccagtatt
    10201 tagaagactt taagctaacg caccacaatc aaagtgcgtt gaaaagcaca ggagtgacct
    10261 tgtgtggtgc cgagcgaaag tcgatggttc gtgaacttag acaattatgt gtcggcttag
    10321 gtatcaccat agattacgaa catcctgcgg tagaagatgc agagctattg tgcaacgaat
    10381 acgatttagt cgtggtttca aacggcatta atcacaccac gacttattac cgtgattccc
    10441 tcaaacccaa agtggaattt ggtaaaaacc gctacatgtg gtacggcaca ccaaaaacat
    10501 ttgatgccat gaacttgatc tttaagtcgc actcaaaagg catttttgtc gcacactctt
    10561 ataaatattc aagcacgatg agcacattcg tcgtcgagtg cagtgaagaa acctatcaaa
    10621 aatcggggct tgatgaaatg tcgccgcaag aagcagagaa attcatcgct tctgtctttg
    10681 aaagtgagtt agacggcaat ccggttatgg cgcaagaagg cttaaaatgg cgtaacttta
    10741 tgacgcttag ccatgagaaa gcatatgacg gtaacgttgc tctgctcggt gacgcattgc
    10801 agtcgggtca cttttcaatc ggtcacggta cgaccatggc tgtggttggc gcacaaatct
    10861 tggttaaagc cgttttcgat aaccccgaca atgttgaagc tgcattggct gactttaacc
    10921 agcacgttat gccaatcatg cgcctatttg atcaacatgc aaccatcagc cgcctttggt
    10981 ttgaaacggt agaagacaga atgcacttgt ctacgggcga agtcgctcag agctttgctg
    11041 agcgccgtgg cgagctccca ccattaccgg cagctttagg ccaagcgctt gaaagagccc
    11101 ttgcccgcgg agacaaataa tatggatctg caaaaacaaa agcatccccc taagctacct
    11161 acgcagtgga atagcagtta catttcatac tggcagccaa tgcagccgca agaccatatc
    11221 acgtctggtt attgctggtt tgattacgcc aataatgtct gtcgaataga tgggttgttt
    11281 aacccttggt cggaagaaaa gaccggtcat agattgtgga tgtcggagat catgtacccg
    11341 gcaaccaatg aatcttttaa gtccaaaatc gcttatagcc gtgcgcaaat ggatcaacaa
    11401 agctcgtttc aagagcaagt attaaacgat gaggttgacc cctgccatga gttgatatta
    11461 atgcaagatg tacttgagct atgcaacgcc gagtttatag gcacctgtga agtgatgggc
    11521 actgaagcgg atatttggca ctttgagcgt cctaataata agggaccagc cacttactat
    11581 ttcaaagcgg ataccaatca acttttgcga atggttacag gcgatccaca aaaaatggca
    11641 tcggtacggg atttccccaa ttttaatact cgagaaattg accctgaaat ttttcaaccg
    11701 actccattaa aacaacctga gtaagcactc gagcaccacc accaccacca ctgagatccg
    11761 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
    11821 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
    11881 atatccggat tggcgaatgg gagctttcca gtcgggaaac ctgtcgtgag gctaggtgga
    11941 ggctcagtga gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    12001 acgcgcgggg agaggcggtt tgcgtattgg gcgcatgcat aaaaactgtt gtaattcatt
    12061 aagcattctg ccgacatgga atccatcaca aacggcatga tgaacctgaa tcgccagcgg
    12121 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gacgcacacc gtggaaacgg
    12181 atgaaggcac gaacccagtt gacataagcc tgttcggttc gtaaactgta atgcaagtag
    12241 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
    12301 gtggcggttt tcatggcttg ttatgactgt ttttttgtac agtctatgcc tcgggcatcc
    12361 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
    12421 acgcagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggtggctc
    12481 aagtatgggc atcattcgca catgtaggct cggccctgac caagtcaaat ccatgcgggc
    12541 tgctcttgat cttttcggtc gtgagttcgg agacgtagcc acctactccc aacatcagcc
    12601 ggactccgat tacctcggga acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt
    12661 cgaccaagaa gcggttgttg gcgctctcgc ggcttacgtt ctgcccaagt ttgagcagcc
    12721 gcgtagtgag atctatatct atgatctcgc agtctccgga gagcaccgga ggcagggcat
    12781 tgccaccgcg ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat
    12841 ctacgtgcaa gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat
    12901 acgggaagaa gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca
    12961 agccgagatc ggcttcccgg ccgcggagtt gttcggtaaa ttgtcacaac gccgcaggtg
    13021 gcacttttcg gggaaatgtg cgcgcccgcg ttcctgctgg cgctgggcct gtttctggcg
    13081 ctggacttcc cgctgttccg tcagcagctt ttcgcccacg gccttgatga tcgcggcggc
    13141 cttggcctgc atatcccgat tcaacggccc cagggc
//
