LOCUS       sequence-332900-        9910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Liu P, Liang SQ, Zheng C, Mintzer E, Zhao YG, Ponnienselvan K, Mir 
            A, Sontheimer EJ, Gao G, Flotte TR, Wolfe SA, Xue W
  TITLE     Improved prime editors enable pathogenic allele correction and 
            cancer modelling in adult mice.
  JOURNAL   Nat Commun. 2021 Apr 9;12(1):2121. doi: 10.1038/s41467-021-22295-w.
  PUBMED    33837189
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-04-9"; volume: "12(1):2121. doi"; pages: " 
            10.1038/s41467-021-22295-w"
COMMENT     Sequence Label: SpyCas9 PE2*-3HA
FEATURES             Location/Qualifiers
     source          1..9910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      246..255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             258..284
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             324..344
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(345..2858,2859..2861,2862..4445)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             345..4445
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6672..6761
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             6765..6785
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6794..6811
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6834..6851)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6840..7064
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7135..7151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7135..7151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7148..7170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7159..7175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7183..7189,7190..7207,7208..7213))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7228..7249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7366..7383)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7537..8125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7617..7636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8296..9087,9088..9156))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8919..8938
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9157..9261)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9340..9359)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9531..9910
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgcgccac catggggccc gccgccaagc gcgtgaagct ggacggcggc aaacggacag
      301 ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcgacaag aagtacagca
      361 tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg
      421 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc
      481 tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa
      541 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca
      601 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
      661 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg
      721 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg
      781 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc
      841 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc
      901 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg
      961 acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg
     1021 cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg
     1081 gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga
     1141 gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg
     1201 ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga
     1261 gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg
     1321 agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt
     1381 acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag
     1441 ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg
     1501 aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca
     1561 acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg
     1621 aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc
     1681 gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca
     1741 gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt
     1801 ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg
     1861 tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag
     1921 tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg
     1981 ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg
     2041 actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt
     2101 tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc
     2161 tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg
     2221 aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag
     2281 tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga
     2341 tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg
     2401 gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg
     2461 acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc
     2521 tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc
     2581 tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga
     2641 accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg
     2701 gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc
     2761 agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg
     2821 aactggacat caaccggctg tccgactacg atgtggacgc tatcgtgcct cagagctttc
     2881 tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga
     2941 gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc
     3001 tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg
     3061 gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga
     3121 tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg
     3181 acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc
     3241 ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg
     3301 cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg
     3361 agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc
     3421 aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg aactttttca
     3481 agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg
     3541 gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc
     3601 tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca
     3661 aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg
     3721 accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg
     3781 ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca
     3841 ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct
     3901 acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg
     3961 aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg
     4021 ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg
     4081 gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg
     4141 acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc
     4201 tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg
     4261 agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact
     4321 ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc
     4381 tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag
     4441 gtgactctgg aggatctagc ggaggatcct ctggcagcga gacaccagga acaagcgagt
     4501 cagcaacacc agagagcagt ggcggcagca gcggcggcag cagcacccta aatatagaag
     4561 atgagtatcg gctacatgag acctcaaaag agccagatgt ttctctaggg tccacatggc
     4621 tgtctgattt tcctcaggcc tgggcggaaa ccgggggcat gggactggca gttcgccaag
     4681 ctcctctgat catacctctg aaagcaacct ctacccccgt gtccataaaa caatacccca
     4741 tgtcacaaga agccagactg gggatcaagc cccacataca gagactgttg gaccagggaa
     4801 tactggtacc ctgccagtcc ccctggaaca cgcccctgct acccgttaag aaaccaggga
     4861 ctaatgatta taggcctgtc caggatctga gagaagtcaa caagcgggtg gaagacatcc
     4921 accccaccgt gcccaaccct tacaacctct tgagcgggct cccaccgtcc caccagtggt
     4981 acactgtgct tgatttaaag gatgcctttt tctgcctgag actccacccc accagtcagc
     5041 ctctcttcgc ctttgagtgg agagatccag agatgggaat ctcaggacaa ttgacctgga
     5101 ccagactccc acagggtttc aaaaacagtc ccaccctgtt taatgaggca ctgcacagag
     5161 acctagcaga cttccggatc cagcacccag acttgatcct gctacagtac gtggatgact
     5221 tactgctggc cgccacttct gagctagact gccaacaagg tactcgggcc ctgttacaaa
     5281 ccctagggaa cctcgggtat cgggcctcgg ccaagaaagc ccaaatttgc cagaaacagg
     5341 tcaagtatct ggggtatctt ctaaaagagg gtcagagatg gctgactgag gccagaaaag
     5401 agactgtgat ggggcagcct actccgaaga cccctcgaca actaagggag ttcctaggga
     5461 aggcaggctt ctgtcgcctc ttcatccctg ggtttgcaga aatggcagcc cccctgtacc
     5521 ctctcaccaa accggggact ctgtttaatt ggggcccaga ccaacaaaag gcctatcaag
     5581 aaatcaagca agctcttcta actgccccag ccctggggtt gccagatttg actaagccct
     5641 ttgaactctt tgtcgacgag aagcagggct acgccaaagg tgtcctaacg caaaaactgg
     5701 gaccttggcg tcggccggtg gcctacctgt ccaaaaagct agacccagta gcagctgggt
     5761 ggcccccttg cctacggatg gtagcagcca ttgccgtact gacaaaggat gcaggcaagc
     5821 taaccatggg acagccacta gtcattctgg ccccccatgc agtagaggca ctagtcaaac
     5881 aaccccccga ccgctggctt tccaacgccc ggatgactca ctatcaggcc ttgcttttgg
     5941 acacggaccg ggtccagttc ggaccggtgg tagccctgaa cccggctacg ctgctcccac
     6001 tgcctgagga agggctgcaa cacaactgcc ttgatatcct ggccgaagcc cacggaaccc
     6061 gacccgacct aacggaccag ccgctcccag acgccgacca cacctggtac acggatggaa
     6121 gcagtctctt acaagaggga cagcgtaagg cgggagctgc ggtgaccacc gagaccgagg
     6181 taatctgggc taaagccctg ccagccggga catccgctca gcgggctgaa ctgatagcac
     6241 tcacccaggc cctaaagatg gcagaaggta agaagctaaa tgtttatact gatagccgtt
     6301 atgcttttgc tactgcccat atccatggag aaatatacag aaggcgtggg tggctcacat
     6361 cagaaggcaa agagatcaaa aataaagacg agatcttggc cctactaaaa gccctctttc
     6421 tgcccaaaag acttagcata atccattgtc caggacatca aaagggacac agcgccgagg
     6481 ctagaggcaa ccggatggct gaccaagcgg cccgaaaggc agccatcaca gagactccag
     6541 acacctctac cctcctcata gaaaattcat caccctctgg cggctcaaaa agaaccgccg
     6601 acggcagcga gaagcgcacc gccgacagcc agcacagcac cccccccaag accaagcgca
     6661 aggtggaatt ctacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg
     6721 actatgcagg atcgtatcct tatgacgttc cagattacgc tgagcccaag aagaagagga
     6781 aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac
     6841 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     6901 ggaaggtgcc actcccactg tcctttccta ataaaatgag aaaattgcat cgcattgtct
     6961 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     7021 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     7081 aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7141 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7201 cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7261 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7321 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7381 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7441 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7501 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7561 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7621 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7681 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7741 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7801 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7861 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7921 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7981 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8041 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8101 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacac
     8161 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8221 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8281 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8341 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8401 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8461 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8521 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8581 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8641 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8701 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8761 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8821 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8881 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8941 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9001 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9061 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9121 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9181 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9241 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     9301 gggagatcga tctcccgatc ccctagggtc tactctcagt acaatctgct ctgatgccgc
     9361 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     9421 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     9481 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     9541 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     9601 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     9661 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     9721 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     9781 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     9841 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     9901 tattaccatg
//
