LOCUS       sequence-332916-        5639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5048:Dendra
FEATURES             Location/Qualifiers
     source          1..5639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1765..1788
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2045..2845
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2970..3558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3459..3478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatggtgttc ggtcggtcga agagcaaaaa cctgcctgcg
      301 actgtcgagg gcgcgaacgg cctctccgac gatcccggca ctgccgccaa ggtgctctcg
      361 cacatcatcg agagcagcac acgcgtgcag gcccccgcgg tgcgggccta cgtggaacgg
      421 ctgcgcagcc agcagccgga cgccacaccc gcccagatcg tcagcaggct ggagaagcac
      481 tacctggccg cggtgatggc cagcggcgcg gcggtcggat cggccgcggc gttcccgggg
      541 atcggcacgc tggcggccct gtcggccgtc gcgggcgaga ccgtggtgtt cctcgaggcc
      601 acctcggtgt tcgtgctcgc ggtcgccgag gtgcacggta tccccgccga ccaccgcgaa
      661 cggcgccgtg cgctggtgct ttccgtgctg gtcggcgagg acagcaaacg cgcgctggcc
      721 gacctgctcg ggaccgggcg cacgagcggc gcatggctgt ccgacggcgc agcaacgctg
      781 cccctgcccg cggtgtcgca gctgaactcc cggctggtca ggtacttcgt gaagcggtat
      841 acgctcaagc gcggcgccat ggccttcggc aagctgctgc cggtgggcat cggcgcgctc
      901 atcggcggag tcggcaaccg catgatgggt aagaagatcg tcgccaacgc cgccaaggcg
      961 ttcggcgcac ccccgccgcg gtggccggca acgttgcatg tgctgccgtc cgctcagacc
     1021 aagcttggtg cggccgcggg caacacgcct ggcatcaatc tgatcaagga ggacatgcgt
     1081 gtcaaggtgc atatggaggg caacgtgaac ggtcacgcct tcgtgatcga gggggagggt
     1141 aagggtaagc cttacgaagg gacccagacc gccaacctca ccgtcaagga gggcgcccct
     1201 ctcccattct cgtacgatat cctcactacc gcggtgcatt acgggaaccg cgtgttcacc
     1261 aagtaccccg aggatatccc tgactacttc aagcagagct tcccggaggg atactcctgg
     1321 gagcggacga tgaccttcga agacaagggt atctgtacga tccgcagcga catctcgctg
     1381 gaaggggact gtttcttcca gaacgtccgt ttcaagggga ccaactttcc gccgaacggc
     1441 ccggtcatgc agaagaagac cctcaagtgg gagccgagca cggagaagct gcacgttcgg
     1501 gacggcctcc tggtcggcaa catcaacatg gcgctgctgc tggaaggcgg cggccactac
     1561 ctatgcgact tcaagaccac gtacaaagcg aagaaggtcg tccagctgcc cgacgcgcac
     1621 ttcgtggacc accgcatcga aatcctgggg aacgattcgg actacaacaa ggtcaagttg
     1681 tacgagcacg ctgtggcacg ctatagcccg ctcccgtcgc aggtctggtt cgaaggcggc
     1741 ggcggggaga acctgtattt ccaggactac aaggacgacg acgacaagtg atttaaataa
     1801 ccccttgggg cctctaaacg ggtcttgagg ggttttttgt taactagcgt acgatcgaat
     1861 tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc ccacagaatg
     1921 atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat cagcaaaagg
     1981 ggatgataag tttatcacca ccgactattt gcaacagtgc cgttgatcgt gctatgatcg
     2041 actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac gaatggcgaa aagccgagct
     2101 catcggtcag cttctcaacc ttggggttac ccccggcggt gtgctgctgg tccacagctc
     2161 cttccgtagc gtccggcccc tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc
     2221 tacgctgggt ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc
     2281 gttcgatcct gccacgtcgc ccgttacacc ggaccttgga gttgtctctg acacattctg
     2341 gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc tttgcggcag cggggccaca
     2401 ggcagagcag atcatctctg atccattgcc cctgccacct tactcgcctg caagcccggt
     2461 cgcccgtgtc catgaactcg atgggcaggt acttctcctc ggcgtgggac acgatgccaa
     2521 cacgacgctg catcttgccg agttgatggc aaaggttccc tatggggtgc cgagacactg
     2581 caccattctt caggatggca agttggtacg cgtcgattat ctcgagaatg accactgctg
     2641 tgagcgcttt gccttggcgg acaggtggct caaggagaag agccttcaga aggaaggtcc
     2701 agtcggtcat gcctttgctc ggttgatccg ctcccgcgac attgtggcga cagccctggg
     2761 tcaactgggc cgagatccgt tgatcttcct gcatccgcca gaggcgggat gcgaagaatg
     2821 cgatgccgct cgccagtcga ttggctgagc tcatgagcgg agaacgagat gacgttggag
     2881 gggcaaggtc gcgctgattg ctggggcaac acgggggatc cactagttcc atgagcgtca
     2941 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3001 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3061 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     3121 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3181 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3241 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3301 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3361 ctatgagaaa gcgccacgct tcccgagggg agaaaggcgg acaggtatcc ggtaagcggc
     3421 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3481 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3541 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3601 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3661 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg caacgcgtgc
     3721 ggccgcggta cccggggatc ctctagagtc gaccaccaag ggcaccatct ctgcttgggc
     3781 caccccgttg gccgcagcca gctcgctgag agccgtgaac gacagggcga acgccagccc
     3841 gccgacggcg agggttccga ccgctgcaac tcccggtgca accttgtccc ggtctattct
     3901 cttcactgca ccagctccaa tctggtgtga atgcccctcg tctgttcgcg caggcggggg
     3961 gctctattcg tttgtcagca tcgaaagtag ccagatcagg gatgcgttgc aaccgcgtat
     4021 gcccaggtca gaagagtcgc acaagagttg cagacccctg gaaagaaaaa tggccagagg
     4081 gcgaaaacac cctctgacca gcggagcggg cgacgggaat cgaacccgcg tagctagttt
     4141 ggaagaatgg gtgtctgccg accacatatg ggccggtcaa gataggtttt taccccctct
     4201 cggctgcatc ctctaagtgg aaagaaattg caggtcgtag aagcgcgttg aagcctgaga
     4261 gttgcacagg agttgcaacc cggtagcctt gttcacgacg agaggagacc tagttggcac
     4321 gtcgcggatg gggatcgctg aagactcagc gcagcgggag gatccaagcc tcatacgtca
     4381 acccgcagga cggtgtgagg tactacgcgc tgcagaccta cgacaacaag atggacgccg
     4441 aagcctggct cgcgggcgag aagcggctca tcgagatgga gacctggacc cctccacagg
     4501 accgggcgaa gaaggcagcc gccagcgcca tcacgctgga ggagtacacc cggaagtggc
     4561 tcgtggagcg cgacctcgca gacggcacca gggatctgta cagcgggcac gcggagcgcc
     4621 gcatctaccc ggtgctaggt gaagtggcgg tcacagagat gacgccagct ctggtgcgtg
     4681 cgtggtgggc cgggatgggt aggaagcacc cgactgcccg ccggcatgcc tacaacgtcc
     4741 tccgggcggt gatgaacaca gcggtcgagg acaagctgat cgcagagaac ccgtgccgga
     4801 tcgagcagaa ggcagccgat gagcgcgacg tagaggcgct gacgcctgag gagctggaca
     4861 tcgtcgccgc tgagatcttc gagcactacc ggatcgcggc atacatcctg gcgtggacga
     4921 gcctccggtt cggagagctg atcgagcttc gccgcaagga catcgtggac gacggcatga
     4981 cgatgaagct ccgggtgcgc cgtggcgctt cccgcgtggg gaacaagatc gtcgttggca
     5041 acgccaagac cgtccggtcg aagcgtcctg tgacggttcc gcctcacgtc gcggagatga
     5101 tccgagcgca catgaaggac cgtacgaaga tgaacaaggg ccccgaggca ttcctggtga
     5161 ccacgacgca gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct
     5221 acgccaagat cggtcggccg gaactccgca tccacgacct ccgcgctgtc ggcgctacgt
     5281 tcgccgctca ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt cacacgactc
     5341 ctaggatggc gatgaagtac cagatggcgt ctgaggcccg cgacgaggct atcgctgagg
     5401 cgatgtccaa gctggccaag acctcctgaa acgcaaaaag cccccctccc aaggacactg
     5461 agtcctaaag aggggggttt cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc
     5521 agcaccgccg ctctgtgcgg agacctgggc accagccccg ccgccgccag gagcattgcc
     5581 gttcccgcca gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg ggacgtcga
//
