LOCUS       sequence-332917-        5984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5222:Dendra
FEATURES             Location/Qualifiers
     source          1..5984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2110..2133
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2390..3190
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3315..3903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3804..3823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatgagcctg aacctcggaa tcgtcggttt gccgaacgtc
      301 ggaaaatcga ccttgttcaa cgccttgacg cggaatgacg tgctggcggc caactatccc
      361 ttcgcgacca tcgagccgaa cgagggtgtg gtgccgctgc cggacccgcg cctggacaag
      421 ctcgccgaac tgttcggctc ggagaagacc gtgcccgcgc ctgtgacgtt cgtcgacatc
      481 gcgggcatcg tgaagggcgc gtccgagggt gcgggccttg gcaacaagtt cctcgccaac
      541 atccgcgaat gtgacgccat ctgtcaggtg gtccgggtct tcaacgacga cgacgtggtt
      601 cacgtcgacg ggcgcgtcga ccccaggtcc gacatcgagg tcatcgagac cgagctgatc
      661 ctcgccgaca tgcagaccct cgagaaggcc gtgccgcgcc tggagaagga agcgcgcaac
      721 aacaaggagc gcaaacccgt ccttgaggcc gccgtcgccg cgcaggccgt gttggacagc
      781 ggcaagacgc tcttcgcggg cgccgccgcg gcaggcatcg acgtgacgct gctgcgcgag
      841 ctcaacctga tgaccaccaa accgttcctg tatgtgttca acgccgacga gtcggtgctc
      901 accgacgagg cccgccaggc cgaactccgt gagatggtgg cgcccgccga cgcggtgttc
      961 ctcgacgcca agatcgaggc agaactgctc gagctcgacg acgagtccgc ggccgagctg
     1021 ctggagtcca tcggtcagac cgagcgcgga ctcgacgcgc tcgcacgcgc gggctttcac
     1081 accctcaaac tccagaccta cctcaccgcg gggcccaagg aggcgcgcgc ctggaccatc
     1141 caccagggcg acaccgcacc caaggccgcc ggcgtgatcc actccgactt cgagaagggc
     1201 ttcatcaagg ccgaggtcgt gtcgttcgac gatctggtcg aggccggctc gatggccgcc
     1261 gcgaaggccg cgggcaaggt ccgcatggaa ggcaaggact acgtcatggc cgacggcgat
     1321 gtggtggagt tccgccacgg tggaacgtct gggagcggaa agaaaaagct tggtgcggcc
     1381 gcgggcaaca cgcctggcat caatctgatc aaggaggaca tgcgtgtcaa ggtgcatatg
     1441 gagggcaacg tgaacggtca cgccttcgtg atcgaggggg agggtaaggg taagccttac
     1501 gaagggaccc agaccgccaa cctcaccgtc aaggagggcg cccctctccc attctcgtac
     1561 gatatcctca ctaccgcggt gcattacggg aaccgcgtgt tcaccaagta ccccgaggat
     1621 atccctgact acttcaagca gagcttcccg gagggatact cctgggagcg gacgatgacc
     1681 ttcgaagaca agggtatctg tacgatccgc agcgacatct cgctggaagg ggactgtttc
     1741 ttccagaacg tccgtttcaa ggggaccaac tttccgccga acggcccggt catgcagaag
     1801 aagaccctca agtgggagcc gagcacggag aagctgcacg ttcgggacgg cctcctggtc
     1861 ggcaacatca acatggcgct gctgctggaa ggcggcggcc actacctatg cgacttcaag
     1921 accacgtaca aagcgaagaa ggtcgtccag ctgcccgacg cgcacttcgt ggaccaccgc
     1981 atcgaaatcc tggggaacga ttcggactac aacaaggtca agttgtacga gcacgctgtg
     2041 gcacgctata gcccgctccc gtcgcaggtc tggttcgaag gcggcggcgg ggagaacctg
     2101 tatttccagg actacaagga cgacgacgac aagtgattta aataacccct tggggcctct
     2161 aaacgggtct tgaggggttt tttgttaact agcgtacgat cgaattcctg cagctcacgg
     2221 taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa
     2281 tgccggcctt tgaatgggtt catgtgcagc tccatcagca aaaggggatg ataagtttat
     2341 caccaccgac tatttgcaac agtgccgttg atcgtgctat gatcgactga tgtcatcagc
     2401 ggtggagtgc aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct
     2461 caaccttggg gttacccccg gcggtgtgct gctggtccac agctccttcc gtagcgtccg
     2521 gcccctcgaa gatgggccac ttggactgat cgaggccctg cgtgctacgc tgggtccggg
     2581 agggacgctc gtcatgccct cgtggtcagg tctggacgac gagccgttcg atcctgccac
     2641 gtcgcccgtt acaccggacc ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt
     2701 aaagcgcagc gcccatccat ttgcctttgc ggcagcgggg ccacaggcag agcagatcat
     2761 ctctgatcca ttgcccctgc caccttactc gcctgcaagc ccggtcgccc gtgtccatga
     2821 actcgatggg caggtacttc tcctcggcgt gggacacgat gccaacacga cgctgcatct
     2881 tgccgagttg atggcaaagg ttccctatgg ggtgccgaga cactgcacca ttcttcagga
     2941 tggcaagttg gtacgcgtcg attatctcga gaatgaccac tgctgtgagc gctttgcctt
     3001 ggcggacagg tggctcaagg agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt
     3061 tgctcggttg atccgctccc gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga
     3121 tccgttgatc ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca
     3181 gtcgattggc tgagctcatg agcggagaac gagatgacgt tggaggggca aggtcgcgct
     3241 gattgctggg gcaacacggg ggatccacta gttccatgag cgtcagaccc cgtagaaaag
     3301 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3361 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3421 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     3481 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3541 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3601 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3661 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3721 acgcttcccg aggggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3781 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3841 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3901 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3961 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4021 gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg cgtgcggccg cggtacccgg
     4081 ggatcctcta gagtcgacca ccaagggcac catctctgct tgggccaccc cgttggccgc
     4141 agccagctcg ctgagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt
     4201 tccgaccgct gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc
     4261 tccaatctgg tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt
     4321 cagcatcgaa agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga
     4381 gtcgcacaag agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct
     4441 gaccagcgga gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc
     4501 tgccgaccac atatgggccg gtcaagatag gtttttaccc cctctcggct gcatcctcta
     4561 agtggaaaga aattgcaggt cgtagaagcg cgttgaagcc tgagagttgc acaggagttg
     4621 caacccggta gccttgttca cgacgagagg agacctagtt ggcacgtcgc ggatggggat
     4681 cgctgaagac tcagcgcagc gggaggatcc aagcctcata cgtcaacccg caggacggtg
     4741 tgaggtacta cgcgctgcag acctacgaca acaagatgga cgccgaagcc tggctcgcgg
     4801 gcgagaagcg gctcatcgag atggagacct ggacccctcc acaggaccgg gcgaagaagg
     4861 cagccgccag cgccatcacg ctggaggagt acacccggaa gtggctcgtg gagcgcgacc
     4921 tcgcagacgg caccagggat ctgtacagcg ggcacgcgga gcgccgcatc tacccggtgc
     4981 taggtgaagt ggcggtcaca gagatgacgc cagctctggt gcgtgcgtgg tgggccggga
     5041 tgggtaggaa gcacccgact gcccgccggc atgcctacaa cgtcctccgg gcggtgatga
     5101 acacagcggt cgaggacaag ctgatcgcag agaacccgtg ccggatcgag cagaaggcag
     5161 ccgatgagcg cgacgtagag gcgctgacgc ctgaggagct ggacatcgtc gccgctgaga
     5221 tcttcgagca ctaccggatc gcggcataca tcctggcgtg gacgagcctc cggttcggag
     5281 agctgatcga gcttcgccgc aaggacatcg tggacgacgg catgacgatg aagctccggg
     5341 tgcgccgtgg cgcttcccgc gtggggaaca agatcgtcgt tggcaacgcc aagaccgtcc
     5401 ggtcgaagcg tcctgtgacg gttccgcctc acgtcgcgga gatgatccga gcgcacatga
     5461 aggaccgtac gaagatgaac aagggccccg aggcattcct ggtgaccacg acgcagggca
     5521 accggctgtc gaagtccgcg ttcaccaagt cgctgaagcg tggctacgcc aagatcggtc
     5581 ggccggaact ccgcatccac gacctccgcg ctgtcggcgc tacgttcgcc gctcaggcag
     5641 gtgcgacgac caaggagctg atggcccgtc tcggtcacac gactcctagg atggcgatga
     5701 agtaccagat ggcgtctgag gcccgcgacg aggctatcgc tgaggcgatg tccaagctgg
     5761 ccaagacctc ctgaaacgca aaaagccccc ctcccaagga cactgagtcc taaagagggg
     5821 ggtttcttgt cagtacgcga agaaccacgc ctggccgcga gcgccagcac cgccgctctg
     5881 tgcggagacc tgggcaccag ccccgccgcc gccaggagca ttgccgttcc cgccagaaat
     5941 ctagacggtg accacaacgc gcccgctttg atcggggacg tcga
//
