LOCUS       sequence-332918-        5801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5471:Dendra
FEATURES             Location/Qualifiers
     source          1..5801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1927..1950
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2207..3007
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3132..3720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3621..3640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatgagcacg ccttcgaaag cccctacggt gtcgattcct
      301 cataccgcag tcgtcccggc ggccggtctg ggaactcgtt tcctgccggc aaccaagact
      361 gtgcccaaag aactgttgcc ggtcgtcgac acgcccggta tcgaactcgt cgcggcagag
      421 gccgccgagg ccggcgcgga gcgcctggtc atcgtcacgt ccgagggcaa ggacggtgtc
      481 gtcgcgcact tcgtccaaga cctggtgctg gagggcaccc tggaagctcg cggcaagaag
      541 accatgttgg agaaggtccg gcgcgcgcct gccctgatca aggtcgaatc ggtggtgcag
      601 gccgagccgc tgggcctcgg ccacgccgtg agctgcgtcg agtccgtcct ggctgccgac
      661 gaggacgcca tctcggtgct gctgcccgac gacctggtgt tgcccaccgg cgtgttggag
      721 accatgtcga aggtgcgggc caagcgcggc ggctcggtgc tgtgcgcgat cgaggtgccc
      781 ggcgacaaga tcagcgccta cggtgtcttc gacgtcgaaa ccgtgcccga cgccgccaac
      841 ccgaacgtgc tgcgggtcaa gggcatggtc gagaagccca ggcccgagga cgcgccgtcg
      901 cacttcgccg cggccggccg ttacgtgctg gaccgcgcga tcttcgacgc gctgcgccgc
      961 gttccccgcg gtaccggtgg cgagatccag ctgaccgacg ccatcgcgtt gctgatcgaa
     1021 gaggaccatc ccgtgcacgt ggtggtgcac cgcggtgctc gacacgacct gggaaatccc
     1081 ggcggctacc tgaaggctgc ggttgacttt gcgttggaac gtgacgacta cggcccagaa
     1141 ctgcggcagt ggttggttga gcgactgggc ttggcagaga agaagcttgg tgcggccgcg
     1201 ggcaacacgc ctggcatcaa tctgatcaag gaggacatgc gtgtcaaggt gcatatggag
     1261 ggcaacgtga acggtcacgc cttcgtgatc gagggggagg gtaagggtaa gccttacgaa
     1321 gggacccaga ccgccaacct caccgtcaag gagggcgccc ctctcccatt ctcgtacgat
     1381 atcctcacta ccgcggtgca ttacgggaac cgcgtgttca ccaagtaccc cgaggatatc
     1441 cctgactact tcaagcagag cttcccggag ggatactcct gggagcggac gatgaccttc
     1501 gaagacaagg gtatctgtac gatccgcagc gacatctcgc tggaagggga ctgtttcttc
     1561 cagaacgtcc gtttcaaggg gaccaacttt ccgccgaacg gcccggtcat gcagaagaag
     1621 accctcaagt gggagccgag cacggagaag ctgcacgttc gggacggcct cctggtcggc
     1681 aacatcaaca tggcgctgct gctggaaggc ggcggccact acctatgcga cttcaagacc
     1741 acgtacaaag cgaagaaggt cgtccagctg cccgacgcgc acttcgtgga ccaccgcatc
     1801 gaaatcctgg ggaacgattc ggactacaac aaggtcaagt tgtacgagca cgctgtggca
     1861 cgctatagcc cgctcccgtc gcaggtctgg ttcgaaggcg gcggcgggga gaacctgtat
     1921 ttccaggact acaaggacga cgacgacaag tgatttaaat aaccccttgg ggcctctaaa
     1981 cgggtcttga ggggtttttt gttaactagc gtacgatcga attcctgcag ctcacggtaa
     2041 ctgatgccgt atttgcagta ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc
     2101 cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata agtttatcac
     2161 caccgactat ttgcaacagt gccgttgatc gtgctatgat cgactgatgt catcagcggt
     2221 ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc agcttctcaa
     2281 ccttggggtt acccccggcg gtgtgctgct ggtccacagc tccttccgta gcgtccggcc
     2341 cctcgaagat gggccacttg gactgatcga ggccctgcgt gctacgctgg gtccgggagg
     2401 gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc ctgccacgtc
     2461 gcccgttaca ccggaccttg gagttgtctc tgacacattc tggcgcctgc caaatgtaaa
     2521 gcgcagcgcc catccatttg cctttgcggc agcggggcca caggcagagc agatcatctc
     2581 tgatccattg cccctgccac cttactcgcc tgcaagcccg gtcgcccgtg tccatgaact
     2641 cgatgggcag gtacttctcc tcggcgtggg acacgatgcc aacacgacgc tgcatcttgc
     2701 cgagttgatg gcaaaggttc cctatggggt gccgagacac tgcaccattc ttcaggatgg
     2761 caagttggta cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct ttgccttggc
     2821 ggacaggtgg ctcaaggaga agagccttca gaaggaaggt ccagtcggtc atgcctttgc
     2881 tcggttgatc cgctcccgcg acattgtggc gacagccctg ggtcaactgg gccgagatcc
     2941 gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg ctcgccagtc
     3001 gattggctga gctcatgagc ggagaacgag atgacgttgg aggggcaagg tcgcgctgat
     3061 tgctggggca acacggggga tccactagtt ccatgagcgt cagaccccgt agaaaagatc
     3121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3241 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3541 cttcccgagg ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3841 gataccgctc gccgcagccg aacgaccgag cgcaacgcgt gcggccgcgg tacccgggga
     3901 tcctctagag tcgaccacca agggcaccat ctctgcttgg gccaccccgt tggccgcagc
     3961 cagctcgctg agagccgtga acgacagggc gaacgccagc ccgccgacgg cgagggttcc
     4021 gaccgctgca actcccggtg caaccttgtc ccggtctatt ctcttcactg caccagctcc
     4081 aatctggtgt gaatgcccct cgtctgttcg cgcaggcggg gggctctatt cgtttgtcag
     4141 catcgaaagt agccagatca gggatgcgtt gcaaccgcgt atgcccaggt cagaagagtc
     4201 gcacaagagt tgcagacccc tggaaagaaa aatggccaga gggcgaaaac accctctgac
     4261 cagcggagcg ggcgacggga atcgaacccg cgtagctagt ttggaagaat gggtgtctgc
     4321 cgaccacata tgggccggtc aagataggtt tttaccccct ctcggctgca tcctctaagt
     4381 ggaaagaaat tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca ggagttgcaa
     4441 cccggtagcc ttgttcacga cgagaggaga cctagttggc acgtcgcgga tggggatcgc
     4501 tgaagactca gcgcagcggg aggatccaag cctcatacgt caacccgcag gacggtgtga
     4561 ggtactacgc gctgcagacc tacgacaaca agatggacgc cgaagcctgg ctcgcgggcg
     4621 agaagcggct catcgagatg gagacctgga cccctccaca ggaccgggcg aagaaggcag
     4681 ccgccagcgc catcacgctg gaggagtaca cccggaagtg gctcgtggag cgcgacctcg
     4741 cagacggcac cagggatctg tacagcgggc acgcggagcg ccgcatctac ccggtgctag
     4801 gtgaagtggc ggtcacagag atgacgccag ctctggtgcg tgcgtggtgg gccgggatgg
     4861 gtaggaagca cccgactgcc cgccggcatg cctacaacgt cctccgggcg gtgatgaaca
     4921 cagcggtcga ggacaagctg atcgcagaga acccgtgccg gatcgagcag aaggcagccg
     4981 atgagcgcga cgtagaggcg ctgacgcctg aggagctgga catcgtcgcc gctgagatct
     5041 tcgagcacta ccggatcgcg gcatacatcc tggcgtggac gagcctccgg ttcggagagc
     5101 tgatcgagct tcgccgcaag gacatcgtgg acgacggcat gacgatgaag ctccgggtgc
     5161 gccgtggcgc ttcccgcgtg gggaacaaga tcgtcgttgg caacgccaag accgtccggt
     5221 cgaagcgtcc tgtgacggtt ccgcctcacg tcgcggagat gatccgagcg cacatgaagg
     5281 accgtacgaa gatgaacaag ggccccgagg cattcctggt gaccacgacg cagggcaacc
     5341 ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg ctacgccaag atcggtcggc
     5401 cggaactccg catccacgac ctccgcgctg tcggcgctac gttcgccgct caggcaggtg
     5461 cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac tcctaggatg gcgatgaagt
     5521 accagatggc gtctgaggcc cgcgacgagg ctatcgctga ggcgatgtcc aagctggcca
     5581 agacctcctg aaacgcaaaa agcccccctc ccaaggacac tgagtcctaa agaggggggt
     5641 ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg ccagcaccgc cgctctgtgc
     5701 ggagacctgg gcaccagccc cgccgccgcc aggagcattg ccgttcccgc cagaaatcta
     5761 gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg a
//
