LOCUS       sequence-332919-        5276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5069:Dendra
FEATURES             Location/Qualifiers
     source          1..5276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1402..1425
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1682..2482
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2607..3195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3096..3115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatgttcgcc aacatcgggt ggggcgagat gctcgtgctg
      301 gtgatcgcgg gtctggtgat cctcggcccc gaacgcctgc ccggcgcgat ccggtggacg
      361 tccggtgcgc tgcgccaggc ccgtgactac gtcagcggcg ccaccagcca gctgcgtcag
      421 gatctggggc ccgagttcga cgatctgcgc gagccgctgc aggaactcca gaagctgcgc
      481 ggcatgacgc cgcgcgcggc gatcaccaaa cacctgctcg acggcgacga ttcgttcctc
      541 accggcaagt tcgacgacga gcgtcccaag cagcagccgc agaccccggc cgcgcaggac
      601 aagcccgaag agaagccgga caagcctgcc gggccggtgt tcgaccccga cgcgaccaag
      661 cttggtgcgg ccgcgggcaa cacgcctggc atcaatctga tcaaggagga catgcgtgtc
      721 aaggtgcata tggagggcaa cgtgaacggt cacgccttcg tgatcgaggg ggagggtaag
      781 ggtaagcctt acgaagggac ccagaccgcc aacctcaccg tcaaggaggg cgcccctctc
      841 ccattctcgt acgatatcct cactaccgcg gtgcattacg ggaaccgcgt gttcaccaag
      901 taccccgagg atatccctga ctacttcaag cagagcttcc cggagggata ctcctgggag
      961 cggacgatga ccttcgaaga caagggtatc tgtacgatcc gcagcgacat ctcgctggaa
     1021 ggggactgtt tcttccagaa cgtccgtttc aaggggacca actttccgcc gaacggcccg
     1081 gtcatgcaga agaagaccct caagtgggag ccgagcacgg agaagctgca cgttcgggac
     1141 ggcctcctgg tcggcaacat caacatggcg ctgctgctgg aaggcggcgg ccactaccta
     1201 tgcgacttca agaccacgta caaagcgaag aaggtcgtcc agctgcccga cgcgcacttc
     1261 gtggaccacc gcatcgaaat cctggggaac gattcggact acaacaaggt caagttgtac
     1321 gagcacgctg tggcacgcta tagcccgctc ccgtcgcagg tctggttcga aggcggcggc
     1381 ggggagaacc tgtatttcca ggactacaag gacgacgacg acaagtgatt taaataaccc
     1441 cttggggcct ctaaacgggt cttgaggggt tttttgttaa ctagcgtacg atcgaattcc
     1501 tgcagctcac ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg
     1561 tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga
     1621 tgataagttt atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact
     1681 gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat
     1741 cggtcagctt ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt
     1801 ccgtagcgtc cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctac
     1861 gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt
     1921 cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg
     1981 cctgccaaat gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc
     2041 agagcagatc atctctgatc cattgcccct gccaccttac tcgcctgcaa gcccggtcgc
     2101 ccgtgtccat gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac
     2161 gacgctgcat cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac
     2221 cattcttcag gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga
     2281 gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt
     2341 cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca
     2401 actgggccga gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga
     2461 tgccgctcgc cagtcgattg gctgagctca tgagcggaga acgagatgac gttggagggg
     2521 caaggtcgcg ctgattgctg gggcaacacg ggggatccac tagttccatg agcgtcagac
     2581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     2761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3001 tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca ggtatccggt aagcggcagg
     3061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3301 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc
     3361 cgcggtaccc ggggatcctc tagagtcgac caccaagggc accatctctg cttgggccac
     3421 cccgttggcc gcagccagct cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc
     3481 gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt
     3541 cactgcacca gctccaatct ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct
     3601 ctattcgttt gtcagcatcg aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc
     3661 caggtcagaa gagtcgcaca agagttgcag acccctggaa agaaaaatgg ccagagggcg
     3721 aaaacaccct ctgaccagcg gagcgggcga cgggaatcga acccgcgtag ctagtttgga
     3781 agaatgggtg tctgccgacc acatatgggc cggtcaagat aggtttttac cccctctcgg
     3841 ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt
     3901 gcacaggagt tgcaacccgg tagccttgtt cacgacgaga ggagacctag ttggcacgtc
     3961 gcggatgggg atcgctgaag actcagcgca gcgggaggat ccaagcctca tacgtcaacc
     4021 cgcaggacgg tgtgaggtac tacgcgctgc agacctacga caacaagatg gacgccgaag
     4081 cctggctcgc gggcgagaag cggctcatcg agatggagac ctggacccct ccacaggacc
     4141 gggcgaagaa ggcagccgcc agcgccatca cgctggagga gtacacccgg aagtggctcg
     4201 tggagcgcga cctcgcagac ggcaccaggg atctgtacag cgggcacgcg gagcgccgca
     4261 tctacccggt gctaggtgaa gtggcggtca cagagatgac gccagctctg gtgcgtgcgt
     4321 ggtgggccgg gatgggtagg aagcacccga ctgcccgccg gcatgcctac aacgtcctcc
     4381 gggcggtgat gaacacagcg gtcgaggaca agctgatcgc agagaacccg tgccggatcg
     4441 agcagaaggc agccgatgag cgcgacgtag aggcgctgac gcctgaggag ctggacatcg
     4501 tcgccgctga gatcttcgag cactaccgga tcgcggcata catcctggcg tggacgagcc
     4561 tccggttcgg agagctgatc gagcttcgcc gcaaggacat cgtggacgac ggcatgacga
     4621 tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg
     4681 ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc
     4741 gagcgcacat gaaggaccgt acgaagatga acaagggccc cgaggcattc ctggtgacca
     4801 cgacgcaggg caaccggctg tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg
     4861 ccaagatcgg tcggccggaa ctccgcatcc acgacctccg cgctgtcggc gctacgttcg
     4921 ccgctcaggc aggtgcgacg accaaggagc tgatggcccg tctcggtcac acgactccta
     4981 ggatggcgat gaagtaccag atggcgtctg aggcccgcga cgaggctatc gctgaggcga
     5041 tgtccaagct ggccaagacc tcctgaaacg caaaaagccc ccctcccaag gacactgagt
     5101 cctaaagagg ggggtttctt gtcagtacgc gaagaaccac gcctggccgc gagcgccagc
     5161 accgccgctc tgtgcggaga cctgggcacc agccccgccg ccgccaggag cattgccgtt
     5221 cccgccagaa atctagacgg tgaccacaac gcgcccgctt tgatcgggga cgtcga
//
