LOCUS       sequence-332920-        5705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5248:Dendra
FEATURES             Location/Qualifiers
     source          1..5705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1831..1854
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2111..2911
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3036..3624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3525..3544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatggcacag aaacctgtcc ctaatgcgct gactctcgag
      301 ctcgagcccg tcgtggccgg cgagatgcgc cgtcacctcg actccgagga cctctggtac
      361 gcgcacgact acgtgccgtt cgaccagggc gagaacttcg ccttcctcgg cggcacggac
      421 tgggatccgt cgcaggtgac gctgcccaag agcatcaccg acgccttgga gatcctcctg
      481 atcaccaagg acaacctcgc cggctaccac cgcgagctcg tcgagcactt catcctcgaa
      541 gaacagtggg gccgcttcct cggccgctgg accgctgagg agcacctgca cgccgtcgcg
      601 ctgcgcaact acctggtggt gacccgcgag atcgatccgg ccgccaacga ggacgtgcgc
      661 gtcgagcacg tcatgaaggg ctaccgcgcc gaccgcttca gccagatcga gacgctggtg
      721 ttcatggcgt tcttcgagcg ctcgcacgcc gtgttctgcc gcaacctgca ggcgcagatc
      781 accgagccgg tcctggcgag cctgatgggc cgcatcgcga ccgacgagga gcgtcacgag
      841 gagttcttct ccaacctcgt cagctacgtg ctggacaagc accgcgacga gaccgtcgag
      901 gccatcgcgg cccgcgcggc gggcctcgac gtgatcggcg ccgacatcgc gccgtaccag
      961 gacaaggtcg ccaacgtcgc cgaggcgggc atcttcgaca aggccgcgct gcagaaggtg
     1021 atctccgacc gcatcacggc atggggcctg gccggcgagg cttcgctgaa ggacttcgtc
     1081 ctggccaagc ttggtgcggc cgcgggcaac acgcctggca tcaatctgat caaggaggac
     1141 atgcgtgtca aggtgcatat ggagggcaac gtgaacggtc acgccttcgt gatcgagggg
     1201 gagggtaagg gtaagcctta cgaagggacc cagaccgcca acctcaccgt caaggagggc
     1261 gcccctctcc cattctcgta cgatatcctc actaccgcgg tgcattacgg gaaccgcgtg
     1321 ttcaccaagt accccgagga tatccctgac tacttcaagc agagcttccc ggagggatac
     1381 tcctgggagc ggacgatgac cttcgaagac aagggtatct gtacgatccg cagcgacatc
     1441 tcgctggaag gggactgttt cttccagaac gtccgtttca aggggaccaa ctttccgccg
     1501 aacggcccgg tcatgcagaa gaagaccctc aagtgggagc cgagcacgga gaagctgcac
     1561 gttcgggacg gcctcctggt cggcaacatc aacatggcgc tgctgctgga aggcggcggc
     1621 cactacctat gcgacttcaa gaccacgtac aaagcgaaga aggtcgtcca gctgcccgac
     1681 gcgcacttcg tggaccaccg catcgaaatc ctggggaacg attcggacta caacaaggtc
     1741 aagttgtacg agcacgctgt ggcacgctat agcccgctcc cgtcgcaggt ctggttcgaa
     1801 ggcggcggcg gggagaacct gtatttccag gactacaagg acgacgacga caagtgattt
     1861 aaataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgttaac tagcgtacga
     1921 tcgaattcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg tacggcccac
     1981 agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag ctccatcagc
     2041 aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta
     2101 tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc
     2161 cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca
     2221 cagctccttc cgtagcgtcc ggcccctcga agatgggcca cttggactga tcgaggccct
     2281 gcgtgctacg ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag gtctggacga
     2341 cgagccgttc gatcctgcca cgtcgcccgt tacaccggac cttggagttg tctctgacac
     2401 attctggcgc ctgccaaatg taaagcgcag cgcccatcca tttgcctttg cggcagcggg
     2461 gccacaggca gagcagatca tctctgatcc attgcccctg ccaccttact cgcctgcaag
     2521 cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg tgggacacga
     2581 tgccaacacg acgctgcatc ttgccgagtt gatggcaaag gttccctatg gggtgccgag
     2641 acactgcacc attcttcagg atggcaagtt ggtacgcgtc gattatctcg agaatgacca
     2701 ctgctgtgag cgctttgcct tggcggacag gtggctcaag gagaagagcc ttcagaagga
     2761 aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg tggcgacagc
     2821 cctgggtcaa ctgggccgag atccgttgat cttcctgcat ccgccagagg cgggatgcga
     2881 agaatgcgat gccgctcgcc agtcgattgg ctgagctcat gagcggagaa cgagatgacg
     2941 ttggaggggc aaggtcgcgc tgattgctgg ggcaacacgg gggatccact agttccatga
     3001 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3061 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3121 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3181 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3241 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3301 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3361 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3421 cgtgagctat gagaaagcgc cacgcttccc gaggggagaa aggcggacag gtatccggta
     3481 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3541 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3601 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3661 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3721 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcaac
     3781 gcgtgcggcc gcggtacccg gggatcctct agagtcgacc accaagggca ccatctctgc
     3841 ttgggccacc ccgttggccg cagccagctc gctgagagcc gtgaacgaca gggcgaacgc
     3901 cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct tgtcccggtc
     3961 tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg ttcgcgcagg
     4021 cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg cgttgcaacc
     4081 gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa gaaaaatggc
     4141 cagagggcga aaacaccctc tgaccagcgg agcgggcgac gggaatcgaa cccgcgtagc
     4201 tagtttggaa gaatgggtgt ctgccgacca catatgggcc ggtcaagata ggtttttacc
     4261 ccctctcggc tgcatcctct aagtggaaag aaattgcagg tcgtagaagc gcgttgaagc
     4321 ctgagagttg cacaggagtt gcaacccggt agccttgttc acgacgagag gagacctagt
     4381 tggcacgtcg cggatgggga tcgctgaaga ctcagcgcag cgggaggatc caagcctcat
     4441 acgtcaaccc gcaggacggt gtgaggtact acgcgctgca gacctacgac aacaagatgg
     4501 acgccgaagc ctggctcgcg ggcgagaagc ggctcatcga gatggagacc tggacccctc
     4561 cacaggaccg ggcgaagaag gcagccgcca gcgccatcac gctggaggag tacacccgga
     4621 agtggctcgt ggagcgcgac ctcgcagacg gcaccaggga tctgtacagc gggcacgcgg
     4681 agcgccgcat ctacccggtg ctaggtgaag tggcggtcac agagatgacg ccagctctgg
     4741 tgcgtgcgtg gtgggccggg atgggtagga agcacccgac tgcccgccgg catgcctaca
     4801 acgtcctccg ggcggtgatg aacacagcgg tcgaggacaa gctgatcgca gagaacccgt
     4861 gccggatcga gcagaaggca gccgatgagc gcgacgtaga ggcgctgacg cctgaggagc
     4921 tggacatcgt cgccgctgag atcttcgagc actaccggat cgcggcatac atcctggcgt
     4981 ggacgagcct ccggttcgga gagctgatcg agcttcgccg caaggacatc gtggacgacg
     5041 gcatgacgat gaagctccgg gtgcgccgtg gcgcttcccg cgtggggaac aagatcgtcg
     5101 ttggcaacgc caagaccgtc cggtcgaagc gtcctgtgac ggttccgcct cacgtcgcgg
     5161 agatgatccg agcgcacatg aaggaccgta cgaagatgaa caagggcccc gaggcattcc
     5221 tggtgaccac gacgcagggc aaccggctgt cgaagtccgc gttcaccaag tcgctgaagc
     5281 gtggctacgc caagatcggt cggccggaac tccgcatcca cgacctccgc gctgtcggcg
     5341 ctacgttcgc cgctcaggca ggtgcgacga ccaaggagct gatggcccgt ctcggtcaca
     5401 cgactcctag gatggcgatg aagtaccaga tggcgtctga ggcccgcgac gaggctatcg
     5461 ctgaggcgat gtccaagctg gccaagacct cctgaaacgc aaaaagcccc cctcccaagg
     5521 acactgagtc ctaaagaggg gggtttcttg tcagtacgcg aagaaccacg cctggccgcg
     5581 agcgccagca ccgccgctct gtgcggagac ctgggcacca gccccgccgc cgccaggagc
     5641 attgccgttc ccgccagaaa tctagacggt gaccacaacg cgcccgcttt gatcggggac
     5701 gtcga
//
