LOCUS       sequence-33293-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of humanized ChR2(H134R) fused to mCherry driven by 
            CaMKIIa promoter for optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  TITLE     hChR2 plasmids
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-CaMKIIa-hChR2(H134R)-mCherry
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      428..437
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgttgctca ggccggtgag gttgctcagg cggataagga tgacaggaca agtgagcagc
       61 cactctgcat agcgcagcca ctgcacccgg tgtcctgtgg caaggtagag catagaggga
      121 ttcttaaact caaaaaagaa ctcgagaatc accttaacca tttcaatggc gcacacatag
      181 atctcctccc agccgcatgt agatttccag gtttggtagg catagaacat cagcagcaaa
      241 atgctgaatc ctgctgcaag ccactgcagg acatttgacg cggtctgagc gccgttcgtg
      301 ccgcgagatt caatccatcc ggcacagtaa cattgatcct cagggaccag gacggacccg
      361 ttcaccacca caggattagt aacgaacaaa agttcgcgtc cgacggcaga caaagcgccg
      421 ccatagtcca tggtggccta ccggtacccg gggatccggt accgctctag agctgccccc
      481 agaactaggg gccactcgcc tgcccgtgct cctgagtgca aacggagaac cggcttgact
      541 gacgagcttg gggcttctga gcagggcact gtggctgctc acagcctcgc gagccttcgt
      601 cagcatccag gtccccatgg caaccacctc cgaaacgccc tgttcccgtt gccccggtaa
      661 ctgcctcccc aacacctgcc tgcc
//
