LOCUS       sequence-332955-       11302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal aid-5Myc-spo5DSR degron cassette with kanMX6 marker for 
            S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11302)
  TITLE     Carr lab yeast expression plasmids
REFERENCE   2  (bases 1 to 11302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAW8-aid-5Myc-spo5DSR-Turg1:kanMX6
FEATURES             Location/Qualifiers
     source          1..11302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..33
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17..33
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(65..98)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             137..823
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             839..868
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             875..904
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             911..940
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             956..985
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             992..1021
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     gene            1780..3136
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1780..2123
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2124..2933
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2191..2210)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2939..3136
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3231..3248)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3231..3247)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3240..3262)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6142..7236
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      8068..8849
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(8944..8963)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9063..9085
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9123..9141)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9209..9313
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9314..9382,9383..10174)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9532..9551)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10345..10933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10834..10853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11087..11104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11221..11242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11257..11262,11263..11280,11281..11287)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(11295..11302,1..9)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(11300..11302,1..20)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatggcgg
       61 ccgcataact tcgtatagca tacattatac gaagttatgc atgctcggtg ggtcaggtgg
      121 aagtggatct ggtgctatga tgggcagtgt cgagctgaat ctgagggaga ctgagctgtg
      181 tcttggtctt cccggtggag atacagtggc tccggtaacc ggaaacaaga gagggttctc
      241 agagacggtt gatctgaagc taaatctgaa taatgagcct gcaaacaagg aaggatctac
      301 gactcatgac gtcgtgactt ttgattccaa ggagaagagt gcttgtccta aagatccagc
      361 caaacctccg gccaaggcac aagttgtggg atggccaccg gtgagatcat accggaagaa
      421 cgtgatggtt tcctgccaaa aatcaagcgg tggcccggag gcggcggcgt tcgtgaaggt
      481 atcaatggac ggagcaccgt acttgaggaa aatcgatttg aggatgtata aaagctacga
      541 tgagctttct aatgctttgt ccaacatgtt cagctctttt accatgggca aacatggagg
      601 agaagaagga atgatagact tcatgaatga gaggaaattg atggatttgg tgaatagctg
      661 ggactatgtt ccctcttatg aagacaaaga cggtgattgg atgctcgtcg gcgacgttcc
      721 ttggccaatg ttcgtcgata catgcaagcg tttacgtctc atgaaaggat cggatgccat
      781 tggtctcgct ccgagggcga tggagaagtg caagagcaga gctggagcag gcgccggtga
      841 acaaaagttg atttctgaag aagatttgaa cggtgaacaa aagctaatct ccgaggaaga
      901 cttgaacggt gaacaaaaat taatctcaga agaagacttg aacggactcg acggtgaaca
      961 aaagttgatt tctgaagaag atttgaacgg tgaacaaaag ctaatctccg aggaagactt
     1021 gtaaggcgcg ccgctagcac tacgccatat catgcccatc ccgctacttc tgctgctaat
     1081 taaatttaaa catctttcca gcatttactt gaacatcaaa ctctaatatc gaaatctttt
     1141 caaacactta tcacatactc aaacttaaac caacataaaa cctgttagac aaagccccgg
     1201 ggcttgccca tctgttttag acgtcacaga aggaccatgt ttattttaaa tttcacatac
     1261 atgtgtttaa ttaccttcat tgtataaatt ctcgaaactt tctccaattc ttcggtcaga
     1321 taaaaaattt ctctagtcac cgtcttttat agagcttttg tcagatgttt ttctggatgg
     1381 aatcattgtc cagttactat gctttttatt tataagtttg aaagtttagt taactttttg
     1441 ttttctttaa tacatttttt aatcatgtca acatttggga tttgtagaag tagtaaattt
     1501 tttgtacatg ttgtagaatt ggataagaac gaaaacagct agtatgtaaa aaaaacgaaa
     1561 atcccaaatt ccgttgtttg tttgatatag taatatttat agcattttag tttctaaaga
     1621 tttttgataa tcttttacat ttgctttcac atacattcgc gctactacca tacatcatat
     1681 cccgctttct ttaattgctt ccccacagac tcaaccagcg gggccttctt ctcgagatct
     1741 gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc
     1801 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt
     1861 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg
     1921 cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca
     1981 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg
     2041 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac
     2101 atcacatccg aacataaaca accatgggta aggaaaagac tcacgtttcg aggccgcgat
     2161 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     2221 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga
     2281 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     2341 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     2401 ggttactcac cactgcgatc cccggcaaaa cagcattcca ggtattagaa gaatatcctg
     2461 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     2521 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     2581 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     2641 ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca
     2701 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     2761 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     2821 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     2881 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagtac
     2941 tgacaataaa aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt
     3001 agttgttcta ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat
     3061 ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat
     3121 gctggtcgct atactgctgt cgattcgata ctaacgccgc catccagtcg acgaactagt
     3181 ataacttcgt atatggtatt atatacgaag ttatgcggcc gcgcgaattc actggccgtc
     3241 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     3301 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     3361 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     3421 tgcggtattt cacaccgcat accatcgtcc ggatcaatga agtagatgag tttactacct
     3481 gtatatactt ttttttttgt caaagtcact tttatggcat ttccatgttg aaggattagc
     3541 cacagcacta aattttcagc gtatgattgc ttttaaatat ttaattttca tcgtttttta
     3601 attaatattc aaaacgattt aatgccttct acatctgaag tcaagctcat agactcgggt
     3661 tgggtagcta aaactacatc taacacgaag gggtatagcc ttcttaaagt atactcatgt
     3721 ataaacgttt tgggttcata tttttgggcc acgtggttta taaaaattct taactacacc
     3781 actcggtatc ccgcacccgt ctacgtttct acgatttcga gctaatattg agtaaagtga
     3841 ttagcaaaaa aaataaaagt actcgttgtc ggagatcaag aatttttcta ttatctcatc
     3901 taaaccactt tctaaaagcg aaaaacaaaa tcgtaatatg cagcttgaat gggcttccat
     3961 agtttgaaag aaaaacccta gcagtactgg caagggagac attcctttta cctggctaat
     4021 cgccatcttc cagcaggcgc accattgccc ctgtttcact atccaggtta cggatatagt
     4081 tcatgacaat atttacattg gtccagccac cagcttgcat gatctccggt attgaaactc
     4141 cagcgcgggc catatctcgc gcggctccga cacgggcact gtgtccagac caggccaggt
     4201 atctctgacc agagtcatcc ttagcgccgt aaatcaatcg atgagttgct tcaaaaatcc
     4261 cttccagggc gcgagttgat agctggctgg tggcagatgg cgcggcaaca ccattttttc
     4321 tgacccggca aaacaggtag ttattcggat catcagctac accagagacg gaaatccatc
     4381 gctcgaccag tttagttacc cccaggctaa gtgccttctc tacacctgcg gtgctaacca
     4441 gcgttttcgt tctgccaata tggattaaca ttctcccacc gtcagtacgt gagatatctt
     4501 taaccctgat cctggcaatt tcggctatac gtaacagggt gttataagca atccccagaa
     4561 atgccagatt acgtatatcc tggcagcgat cgctattttc catgagtgaa cgaacctggt
     4621 cgaaatcagt gcgttcgaac gctagagcct gttttgcacg ttcaccggca tcaacgtttt
     4681 cttttcggat ccgccgcata accagtgaaa cagcattgct gtcacttggt cgtggcagcc
     4741 cggaccgacg atgaagcatg tttagctggc ccaaatgttg ctggatagtt tttactgcca
     4801 gaccgcgcgc ctgaagatat agaagataat cgcgaacatc ttcaggttct gcgggaaacc
     4861 atttccggtt attcaacttg caccatgccg cccacgaccg gcaaacggac agaagcattt
     4921 tccaggtatg ctcagaaaac gcctggcgat ccctgaacat gtccatcagg ttcttgcgaa
     4981 cctcatcact cgttgcatcg accggtaact tttagattaa catttgttta aatatgaaat
     5041 taggcaatgt aacatactgc aggcaaattt tggtgtacgg taagtaaatt ggacatatga
     5101 tttaacaaag cgactataag tcagaaagtg agaatgagat tgaaataatt aattcaactt
     5161 attcaattga tgatatgcca ggattcctct tcctttatct tcaatgtttc gattaacagg
     5221 tccattatcc ggtttttagt ttcgctgcac attgccgaat gacaatcgga ttcctttaag
     5281 ctttgctttt gccctgtttt ctctccccag taatcgttgg gtgactgaac catccagtag
     5341 ccgagagtta cgtttttcct gatttaataa atatccatat ctatttgtct cgtggtgttt
     5401 tacagcaact aaccgaaatt actattttgc atcatccaac cataaagaga caacaataat
     5461 tctagaaaac atataacaag tgtttttttc tatttacgtc tcatacgcaa agtccacttt
     5521 accatgtttt ggaaacttgt acccaaccat tcatttttcg attttgtaat gaggctcttt
     5581 tcttctaaca atcattgagg tcattatcat gtgtcctcac ctattgttta aattgaggct
     5641 tgcgctattc ccaaaattgt gggtattatt ttcatatatc agtatctgca atttcggttc
     5701 aactccggaa gctcggtacc caactgtggg aatactcagg tatcgtaaga tgcaagagtt
     5761 cgaatctctt agcaaccatt attttttttc tctcaacata acgagaacac acaggggcgc
     5821 tatcgcacag aatcaaattc gatgactgga aattttttgt taatttcaga ggtcgcctga
     5881 cgcatatacc tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac
     5941 cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga
     6001 agttgacaat attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt
     6061 actttctaac ttttcttacc ttttacattt cagcaatata tatatatata tatatatata
     6121 tttcaaggat ataccattgt aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac
     6181 cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat ttctgatgtt
     6241 cgttccaatg tcaagttcga tttcgaaaat catttaattg gtggtgctgc tatcgatgct
     6301 acaggtgttc cacttccaga tgaggcgctg gaagcctcca agaaggctga tgccgttttg
     6361 ttaggtgctg tgggtggtcc taaatggggt accggtagtg ttagacctga acaaggttta
     6421 ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc
     6481 gactctcttt tagacttatc tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt
     6541 gttgttagag aattagtggg aggtatttac tttggtaaga gaaaggaaga cgatggtgat
     6601 ggtgtcgctt gggatagtga acaatacacc gttccagaag tgcaaagaat cacaagaatg
     6661 gccgctttca tggccctaca acatgagcca ccattgccta tttggtcctt ggataaagct
     6721 aatgttttgg cctcttcaag attatggaga aaaactgtgg aggaaaccat caagaacgaa
     6781 tttcctacat tgaaagttca acatcaattg attgattctg ccgccatgat cctagttaag
     6841 aacccaaccc acctaaatgg tattataatc accagcaaca tgtttggtga tatcatctcc
     6901 gatgaagcct ccgttatccc aggctccttg ggtttgttgc catctgcgtc cttggcctct
     6961 ttgccagaca agaacaccgc atttggtttg tacgaaccat gccatggttc cgctccagat
     7021 ttgccaaaga ataaggtcaa ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa
     7081 ttgtcattga acttgcctga agaaggtaaa gccattgaag atgcagttaa aaaggttttg
     7141 gatgcaggta tcagaactgg tgatttaggt ggttccaaca gtaccaccga agtcggtgat
     7201 gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag attctctttt tttgtgatat
     7261 ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt acaaaatgga
     7321 atatgttcat agggtagacg aaactatata cgcaatctac atacatttat caagaaggag
     7381 aaaaaggagg atgtaaagga atacaggtaa gcaaattgat actaatggct caacgtgata
     7441 aggaaaaaga attgcacttt aacattaata ttgacaagga ggagggcatc acacaaaaag
     7501 ttaggtgtaa cagaaaatca tgaaactatg atttctaatt tatatattgg aggattttct
     7561 ctaaaaaaaa aaatacaaca aataaaaaac actcaatgac ctgaccattt gatggagttt
     7621 aagtcaatac cttcttgaac catttcccat aatggtgaaa gttccctcaa gaattttact
     7681 ctgtcagaaa cggccttaac gacgtagtcg atccggacga tggccaacct tccaattcat
     7741 taaatcgagt cctccatcag atgatttaca aggttttgca tagaatccct aagtaagaga
     7801 agttgttagt gatttcttca agtttactaa aatttacata caaatggatg atctcttctc
     7861 cattgtttcc tttcttcttg caaacgcgtt ttacaaagag atgacatgct attttctgat
     7921 tatttttttc tatagttttc tattttgtac gtccttggga gtccgaaatg taaaatcggg
     7981 gtatggttag tagcggcagc gttcattatg gtatataaaa gataaaaagt aggggcaagc
     8041 gagacagaat gggatacaag ggcatcgtct ataattatag ctaaaaattg tattttaatt
     8101 tgtatttttt gtaattttat ttctcattgt tttacttaaa atgaatgcga attagagaaa
     8161 acttcaacga aatgtcaaaa taagctcagc aaaatataca attttaggga aagcgatcag
     8221 caacttattc tccgttgtag attctttttc ttcataaatt ccaacaatgt aactgccaat
     8281 tgatttttct ctagatgcta ataaattgaa actaatgaat ataataacac aggttttgat
     8341 aaattgtaaa ctcttactca aaaaaagaat gttaaaacta atttaacctt tttataaagg
     8401 ctcatggcgt taaagaggat agtaaaaatt ctacaatttt ttaatttaat gtcacaattg
     8461 tcttgcgaat ataattctat taattttcgc taactgctgt tttagtacta ttactttcaa
     8521 tatgccttat atgcaacttt aattcatgaa gaatgcaata tttttgcaat ctttcttgta
     8581 ctgagcgctt tttatcattt agtactcatt atttaatttt tcagtaaagg gcaataaata
     8641 aatttgtaga agatgcaatg taatctcctc tatccttttg ctcatatgtt tatgagtata
     8701 cctagtctag aaaggcttgt atttaaaata ttattcaata aaaattcaca atttttacga
     8761 catgtgctac cattcactta acttcctgat tataaaattg gttcgtttat actaattact
     8821 taagtacctt taactaaaca aaatgcctat atatatatta atttacaatg agtgtcagat
     8881 aagtcactat gtccgagtgg ttaaggagtt agactcgcca tcgatggtat ggtgcactct
     8941 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     9001 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     9061 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     9121 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     9181 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     9241 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9301 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     9361 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     9421 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     9481 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     9541 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     9601 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     9661 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9721 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9781 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9841 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9901 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9961 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    10021 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    10081 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    10141 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    10201 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10261 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10321 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10381 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10441 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10501 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10561 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10621 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10681 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10741 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10801 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10861 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10921 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10981 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    11041 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    11101 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    11161 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    11221 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    11281 tatgttgtgt ggaattgtga gc
//
