LOCUS       sequence-33296-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of humanized ChR2 with H134R mutation fused to 
            mCherry, for optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  TITLE     hChR2 plasmids
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-hSyn-hChR2(H134R)-mCherry
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      428..437
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgttgctca ggccggtgag gttgctcagg cggataagga tgacaggaca agtgagcagc
       61 cactctgcat agcgcagcca ctgcacccgg tgtcctgtgg caaggtagag catagaggga
      121 ttcttaaact caaaaaagaa ctcgagaatc accttaacca tttcaatggc gcacacatag
      181 atctcctccc agccgcatgt agatttccag gtttggtagg catagaacat cagcagcaaa
      241 atgctgaatc ctgctgcaag ccactgcagg acatttgacg cggtctgagc gccgttcgtg
      301 ccgcgagatt caatccatcc ggcacagtaa cattgatcct cagggaccag gacggacccg
      361 ttcaccacca caggattagt aacgaacaaa agttcgcgtc cgacggcaga caaagcgccg
      421 ccatagtcca tggtggcgtc gactctagag gatccggtac cttctcgact gcgctctcag
      481 gcacgacacg actcctccgc tgcccaccgc agactgaggc agcgctgagt cgccggcgcc
      541 gcagcgcaga tggtcgcgcc cgtgcccccc tatctcgcgc ctcgcgtggt gcggtccggc
      601 tgggccggcg gcggcgcgga cgcgaccaag gtggccggga aggggagttt gcgggggacc
      661 ggcgagtgac gtcagcgcgc cttcagtgct ga
//
