LOCUS       sequence-332978-       11303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal aid-5Myc-eDSR degron cassette with kanMX6 marker for S. 
            pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11303)
  TITLE     Carr lab yeast expression plasmids
REFERENCE   2  (bases 1 to 11303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAW8-aid-5Myc-eDSR-Turg1:kanMX6
FEATURES             Location/Qualifiers
     source          1..11303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1873..2654
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(2749..2768)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2868..2890
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2928..2946)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3014..3118
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3119..3187,3188..3979)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3337..3356)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4150..4738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4639..4658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4892..4909
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5026..5047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5062..5067,5068..5085,5086..5092)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5100..5116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5105..5127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5124..5140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5124..5140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5172..5205)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5244..5930
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             5946..5975
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5982..6011
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6018..6047
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6063..6092
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6099..6128
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     gene            6888..8244
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        6888..7231
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7232..8041
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7299..7318)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      8047..8244
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8339..8356)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8339..8355)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8348..8370)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             join(11250..11303,1..1041)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 atcacagccg aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag
       61 ttcgatttcg aaaatcattt aattggtggt gctgctatcg atgctacagg tgttccactt
      121 ccagatgagg cgctggaagc ctccaagaag gctgatgccg ttttgttagg tgctgtgggt
      181 ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa
      241 gaacttcaat tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac
      301 ttatctccaa tcaagccaca atttgctaaa ggtactgact tcgttgttgt tagagaatta
      361 gtgggaggta tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat
      421 agtgaacaat acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc
      481 ctacaacatg agccaccatt gcctatttgg tccttggata aagctaatgt tttggcctct
      541 tcaagattat ggagaaaaac tgtggaggaa accatcaaga acgaatttcc tacattgaaa
      601 gttcaacatc aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta
      661 aatggtatta taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt
      721 atcccaggct ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac
      781 accgcatttg gtttgtacga accatgccat ggttccgctc cagatttgcc aaagaataag
      841 gtcaacccta tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg
      901 cctgaagaag gtaaagccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga
      961 actggtgatt taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa
     1021 gttaagaaaa tccttgctta aaaagattct ctttttttgt gatatttgta cataaacttt
     1081 ataaatgaaa ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt
     1141 agacgaaact atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatgta
     1201 aaggaataca ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa aaagaattgc
     1261 actttaacat taatattgac aaggaggagg gcatcacaca aaaagttagg tgtaacagaa
     1321 aatcatgaaa ctatgatttc taatttatat attggaggat tttctctaaa aaaaaaaata
     1381 caacaaataa aaaacactca atgacctgac catttgatgg agtttaagtc aataccttct
     1441 tgaaccattt cccataatgg tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc
     1501 ttaacgacgt agtcgatccg gacgatggcc aaccttccaa ttcattaaat cgagtcctcc
     1561 atcagatgat ttacaaggtt ttgcatagaa tccctaagta agagaagttg ttagtgattt
     1621 cttcaagttt actaaaattt acatacaaat ggatgatctc ttctccattg tttcctttct
     1681 tcttgcaaac gcgttttaca aagagatgac atgctatttt ctgattattt ttttctatag
     1741 ttttctattt tgtacgtcct tgggagtccg aaatgtaaaa tcggggtatg gttagtagcg
     1801 gcagcgttca ttatggtata taaaagataa aaagtagggg caagcgagac agaatgggat
     1861 acaagggcat cgtctataat tatagctaaa aattgtattt taatttgtat tttttgtaat
     1921 tttatttctc attgttttac ttaaaatgaa tgcgaattag agaaaacttc aacgaaatgt
     1981 caaaataagc tcagcaaaat atacaatttt agggaaagcg atcagcaact tattctccgt
     2041 tgtagattct ttttcttcat aaattccaac aatgtaactg ccaattgatt tttctctaga
     2101 tgctaataaa ttgaaactaa tgaatataat aacacaggtt ttgataaatt gtaaactctt
     2161 actcaaaaaa agaatgttaa aactaattta acctttttat aaaggctcat ggcgttaaag
     2221 aggatagtaa aaattctaca attttttaat ttaatgtcac aattgtcttg cgaatataat
     2281 tctattaatt ttcgctaact gctgttttag tactattact ttcaatatgc cttatatgca
     2341 actttaattc atgaagaatg caatattttt gcaatctttc ttgtactgag cgctttttat
     2401 catttagtac tcattattta atttttcagt aaagggcaat aaataaattt gtagaagatg
     2461 caatgtaatc tcctctatcc ttttgctcat atgtttatga gtatacctag tctagaaagg
     2521 cttgtattta aaatattatt caataaaaat tcacaatttt tacgacatgt gctaccattc
     2581 acttaacttc ctgattataa aattggttcg tttatactaa ttacttaagt acctttaact
     2641 aaacaaaatg cctatatata tattaattta caatgagtgt cagataagtc actatgtccg
     2701 agtggttaag gagttagact cgccatcgat ggtatggtgc actctcagta caatctgctc
     2761 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     2821 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2881 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     2941 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     3001 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3061 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3121 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3181 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3241 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3301 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3361 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3421 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3481 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3541 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3601 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3661 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3721 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3781 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3841 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3901 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3961 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4021 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4081 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4141 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4201 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4261 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4321 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4381 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4441 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4501 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4561 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4621 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4681 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4741 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4801 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4861 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4921 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     4981 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     5041 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     5101 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
     5161 atggcggccg cataacttcg tatagcatac attatacgaa gttatgcatg ctcggtgggt
     5221 caggtggaag tggatctggt gctatgatgg gcagtgtcga gctgaatctg agggagactg
     5281 agctgtgtct tggtcttccc ggtggagata cagtggctcc ggtaaccgga aacaagagag
     5341 ggttctcaga gacggttgat ctgaagctaa atctgaataa tgagcctgca aacaaggaag
     5401 gatctacgac tcatgacgtc gtgacttttg attccaagga gaagagtgct tgtcctaaag
     5461 atccagccaa acctccggcc aaggcacaag ttgtgggatg gccaccggtg agatcatacc
     5521 ggaagaacgt gatggtttcc tgccaaaaat caagcggtgg cccggaggcg gcggcgttcg
     5581 tgaaggtatc aatggacgga gcaccgtact tgaggaaaat cgatttgagg atgtataaaa
     5641 gctacgatga gctttctaat gctttgtcca acatgttcag ctcttttacc atgggcaaac
     5701 atggaggaga agaaggaatg atagacttca tgaatgagag gaaattgatg gatttggtga
     5761 atagctggga ctatgttccc tcttatgaag acaaagacgg tgattggatg ctcgtcggcg
     5821 acgttccttg gccaatgttc gtcgatacat gcaagcgttt acgtctcatg aaaggatcgg
     5881 atgccattgg tctcgctccg agggcgatgg agaagtgcaa gagcagagct ggagcaggcg
     5941 ccggtgaaca aaagttgatt tctgaagaag atttgaacgg tgaacaaaag ctaatctccg
     6001 aggaagactt gaacggtgaa caaaaattaa tctcagaaga agacttgaac ggactcgacg
     6061 gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta atctccgagg
     6121 aagacttgta aggcgcgccg ctagcactac gccatatcat gcccatcccg ctacttctgc
     6181 tgctaattaa actttaaaca tctttccagc atttacttga acatcaaact ctaatatcga
     6241 aatcttttca aacacttatc acatactcaa acttaaacca acattaaacc tgttagacaa
     6301 agccccgggg cttgcccatc tgttttagac gtcacagaag gaccatgttt attttaaatt
     6361 tcacatacat gtgtttaatt accttcattg tataaattct cgaaactttc tccaattctt
     6421 cggtcagata aaaaatttct ctagtcaccg tcttttatag agcttttgtc agatgttttt
     6481 ctggatggaa tcattgtcca gttactatgc tttttattta taagtttgaa agtttagtta
     6541 actttttgtt ttctttaata cattttttaa tcatgtcaac atttgggatt tgtagaagta
     6601 gtaaattttt tgtacatgtt gtagaattgg ataagaacga aaacagctag tatgtaaaaa
     6661 aaacgaaaat cccaaattcc gttgtttgtt tgatatagta atatttatag cattttagtt
     6721 tctaaagatt tttgataatc ttttacattt gctttcacat acattcgcgc tactaccata
     6781 catcatatcc cgctttcttt aattgcttcc ccacagactc aaccagcggg gccttcttct
     6841 cgagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc
     6901 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc
     6961 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc
     7021 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc
     7081 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt
     7141 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca
     7201 gttctcacat cacatccgaa cataaacaac catgggtaag gaaaagactc acgtttcgag
     7261 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     7321 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
     7381 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     7441 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     7501 tgatgcatgg ttactcacca ctgcgatccc cggcaaaaca gcattccagg tattagaaga
     7561 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     7621 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     7681 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     7741 ctggcctgtt gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc
     7801 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     7861 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     7921 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     7981 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     8041 atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt
     8101 tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc
     8161 gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt
     8221 atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtcgac
     8281 gaactagtat aacttcgtat atggtattat atacgaagtt atgcggccgc gcgaattcac
     8341 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     8401 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     8461 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     8521 cgcatctgtg cggtatttca caccgcatac catcgtccgg atcaatgaag tagatgagtt
     8581 tactacctgt atatactttt ttttttgtca aagtcacttt tatggcattt ccatgttgaa
     8641 ggattagcca cagcactaaa ttttcagcgt atgattgctt ttaaatattt aattttcatc
     8701 gttttttaat taatattcaa aacgatttaa tgccttctac atctgaagtc aagctcatag
     8761 actcgggttg ggtagctaaa actacatcta acacgaaggg gtatagcctt cttaaagtat
     8821 actcatgtat aaacgttttg ggttcatatt tttgggccac gtggtttata aaaattctta
     8881 actacaccac tcggtatccc gcacccgtct acgtttctac gatttcgagc taatattgag
     8941 taaagtgatt agcaaaaaaa ataaaagtac tcgttgtcgg agatcaagaa tttttctatt
     9001 atctcatcta aaccactttc taaaagcgaa aaacaaaatc gtaatatgca gcttgaatgg
     9061 gcttccatag tttgaaagaa aaaccctagc agtactggca agggagacat tccttttacc
     9121 tggctaatcg ccatcttcca gcaggcgcac cattgcccct gtttcactat ccaggttacg
     9181 gatatagttc atgacaatat ttacattggt ccagccacca gcttgcatga tctccggtat
     9241 tgaaactcca gcgcgggcca tatctcgcgc ggctccgaca cgggcactgt gtccagacca
     9301 ggccaggtat ctctgaccag agtcatcctt agcgccgtaa atcaatcgat gagttgcttc
     9361 aaaaatccct tccagggcgc gagttgatag ctggctggtg gcagatggcg cggcaacacc
     9421 attttttctg acccggcaaa acaggtagtt attcggatca tcagctacac cagagacgga
     9481 aatccatcgc tcgaccagtt tagttacccc caggctaagt gccttctcta cacctgcggt
     9541 gctaaccagc gttttcgttc tgccaatatg gattaacatt ctcccaccgt cagtacgtga
     9601 gatatcttta accctgatcc tggcaatttc ggctatacgt aacagggtgt tataagcaat
     9661 ccccagaaat gccagattac gtatatcctg gcagcgatcg ctattttcca tgagtgaacg
     9721 aacctggtcg aaatcagtgc gttcgaacgc tagagcctgt tttgcacgtt caccggcatc
     9781 aacgttttct tttcggatcc gccgcataac cagtgaaaca gcattgctgt cacttggtcg
     9841 tggcagcccg gaccgacgat gaagcatgtt tagctggccc aaatgttgct ggatagtttt
     9901 tactgccaga ccgcgcgcct gaagatatag aagataatcg cgaacatctt caggttctgc
     9961 gggaaaccat ttccggttat tcaacttgca ccatgccgcc cacgaccggc aaacggacag
    10021 aagcattttc caggtatgct cagaaaacgc ctggcgatcc ctgaacatgt ccatcaggtt
    10081 cttgcgaacc tcatcactcg ttgcatcgac cggtaacttt tagattaaca tttgtttaaa
    10141 tatgaaatta ggcaatgtaa catactgcag gcaaattttg gtgtacggta agtaaattgg
    10201 acatatgatt taacaaagcg actataagtc agaaagtgag aatgagattg aaataattaa
    10261 ttcaacttat tcaattgatg atatgccagg attcctcttc ctttatcttc aatgtttcga
    10321 ttaacaggtc cattatccgg tttttagttt cgctgcacat tgccgaatga caatcggatt
    10381 cctttaagct ttgcttttgc cctgttttct ctccccagta atcgttgggt gactgaacca
    10441 tccagtagcc gagagttacg tttttcctga tttaataaat atccatatct atttgtctcg
    10501 tggtgtttta cagcaactaa ccgaaattac tattttgcat catccaacca taaagagaca
    10561 acaataattc tagaaaacat ataacaagtg tttttttcta tttacgtctc atacgcaaag
    10621 tccactttac catgttttgg aaacttgtac ccaaccattc atttttcgat tttgtaatga
    10681 ggctcttttc ttctaacaat cattgaggtc attatcatgt gtcctcacct attgtttaaa
    10741 ttgaggcttg cgctattccc aaaattgtgg gtattatttt catatatcag tatctgcaat
    10801 ttcggttcaa ctccggaagc tcggtaccca actgtgggaa tactcaggta tcgtaagatg
    10861 caagagttcg aatctcttag caaccattat tttttttctc tcaacataac gagaacacac
    10921 aggggcgcta tcgcacagaa tcaaattcga tgactggaaa ttttttgtta atttcagagg
    10981 tcgcctgacg catatacctt tttcaactga aaaattggga gaaaaaggaa aggtgagagc
    11041 gccggaaccg gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca
    11101 atacttgaag ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca
    11161 atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata tatatatata
    11221 tatatatatt tcaaggatat accattgtaa tgtctgcccc taagaagatc gtcgttttgc
    11281 caggtgacca cgttggtcaa gaa
//
