LOCUS       sequence-332979-       11222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal aid-3HA-eDSR degron cassette with kanMX6 marker for S. 
            pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11222)
  TITLE     Carr lab yeast expression plasmids
REFERENCE   2  (bases 1 to 11222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAW8-aid-3HA-eDSR-Turg1:kanMX6
FEATURES             Location/Qualifiers
     source          1..11222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             280..1374
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      2206..2987
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(3082..3101)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3201..3223
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3261..3279)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3347..3451
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3452..3520,3521..4312)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3670..3689)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4483..5071
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4972..4991
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5225..5242
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5359..5380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5395..5400,5401..5418,5419..5425)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5433..5449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5438..5460
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5457..5473
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5457..5473
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5505..5538)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5577..6263
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             6282..6371
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     gene            7140..8496
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        7140..7483
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7484..8293
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7551..7570)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      8299..8496
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8591..8608)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8591..8607)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8600..8622)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atttcagagg tcgcctgacg catatacctt tttcaactga aaaattggga gaaaaaggaa
       61 aggtgagagc gccggaaccg gcttttcata tagaatagag aagcgttcat gactaaatgc
      121 ttgcatcaca atacttgaag ttgacaatat tatttaagga cctattgttt tttccaatag
      181 gtggttagca atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata
      241 tatatatata tatatatatt tcaaggatat accattgtaa tgtctgcccc taagaagatc
      301 gtcgttttgc caggtgacca cgttggtcaa gaaatcacag ccgaagccat taaggttctt
      361 aaagctattt ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca tttaattggt
      421 ggtgctgcta tcgatgctac aggtgttcca cttccagatg aggcgctgga agcctccaag
      481 aaggctgatg ccgttttgtt aggtgctgtg ggtggtccta aatggggtac cggtagtgtt
      541 agacctgaac aaggtttact aaaaatccgt aaagaacttc aattgtacgc caacttaaga
      601 ccatgtaact ttgcatccga ctctctttta gacttatctc caatcaagcc acaatttgct
      661 aaaggtactg acttcgttgt tgttagagaa ttagtgggag gtatttactt tggtaagaga
      721 aaggaagacg atggtgatgg tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg
      781 caaagaatca caagaatggc cgctttcatg gccctacaac atgagccacc attgcctatt
      841 tggtccttgg ataaagctaa tgttttggcc tcttcaagat tatggagaaa aactgtggag
      901 gaaaccatca agaacgaatt tcctacattg aaagttcaac atcaattgat tgattctgcc
      961 gccatgatcc tagttaagaa cccaacccac ctaaatggta ttataatcac cagcaacatg
     1021 tttggtgata tcatctccga tgaagcctcc gttatcccag gctccttggg tttgttgcca
     1081 tctgcgtcct tggcctcttt gccagacaag aacaccgcat ttggtttgta cgaaccatgc
     1141 catggttccg ctccagattt gccaaagaat aaggtcaacc ctatcgccac tatcttgtct
     1201 gctgcaatga tgttgaaatt gtcattgaac ttgcctgaag aaggtaaagc cattgaagat
     1261 gcagttaaaa aggttttgga tgcaggtatc agaactggtg atttaggtgg ttccaacagt
     1321 accaccgaag tcggtgatgc tgtcgccgaa gaagttaaga aaatccttgc ttaaaaagat
     1381 tctctttttt tgtgatattt gtacataaac tttataaatg aaattcataa tagaaacgac
     1441 acgaaattac aaaatggaat atgttcatag ggtagacgaa actatatacg caatctacat
     1501 acatttatca agaaggagaa aaaggaggat gtaaaggaat acaggtaagc aaattgatac
     1561 taatggctca acgtgataag gaaaaagaat tgcactttaa cattaatatt gacaaggagg
     1621 agggcatcac acaaaaagtt aggtgtaaca gaaaatcatg aaactatgat ttctaattta
     1681 tatattggag gattttctct aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct
     1741 gaccatttga tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt
     1801 tccctcaaga attttactct gtcagaaacg gccttaacga cgtagtcgat ccggacgatg
     1861 gccaaccttc caattcatta aatcgagtcc tccatcagat gatttacaag gttttgcata
     1921 gaatccctaa gtaagagaag ttgttagtga tttcttcaag tttactaaaa tttacataca
     1981 aatggatgat ctcttctcca ttgtttcctt tcttcttgca aacgcgtttt acaaagagat
     2041 gacatgctat tttctgatta tttttttcta tagttttcta ttttgtacgt ccttgggagt
     2101 ccgaaatgta aaatcggggt atggttagta gcggcagcgt tcattatggt atataaaaga
     2161 taaaaagtag gggcaagcga gacagaatgg gatacaaggg catcgtctat aattatagct
     2221 aaaaattgta ttttaatttg tattttttgt aattttattt ctcattgttt tacttaaaat
     2281 gaatgcgaat tagagaaaac ttcaacgaaa tgtcaaaata agctcagcaa aatatacaat
     2341 tttagggaaa gcgatcagca acttattctc cgttgtagat tctttttctt cataaattcc
     2401 aacaatgtaa ctgccaattg atttttctct agatgctaat aaattgaaac taatgaatat
     2461 aataacacag gttttgataa attgtaaact cttactcaaa aaaagaatgt taaaactaat
     2521 ttaacctttt tataaaggct catggcgtta aagaggatag taaaaattct acaatttttt
     2581 aatttaatgt cacaattgtc ttgcgaatat aattctatta attttcgcta actgctgttt
     2641 tagtactatt actttcaata tgccttatat gcaactttaa ttcatgaaga atgcaatatt
     2701 tttgcaatct ttcttgtact gagcgctttt tatcatttag tactcattat ttaatttttc
     2761 agtaaagggc aataaataaa tttgtagaag atgcaatgta atctcctcta tccttttgct
     2821 catatgttta tgagtatacc tagtctagaa aggcttgtat ttaaaatatt attcaataaa
     2881 aattcacaat ttttacgaca tgtgctacca ttcacttaac ttcctgatta taaaattggt
     2941 tcgtttatac taattactta agtaccttta actaaacaaa atgcctatat atatattaat
     3001 ttacaatgag tgtcagataa gtcactatgt ccgagtggtt aaggagttag actcgccatc
     3061 gatggtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     3121 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     3181 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     3241 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3301 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3361 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3421 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3481 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3541 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3601 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3661 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3721 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3781 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3841 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3901 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3961 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4021 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4081 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4141 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4201 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4261 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4321 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4381 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4441 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4501 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4561 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4621 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4681 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4741 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4801 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4861 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4921 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4981 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5041 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5101 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5161 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5221 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5281 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5341 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     5401 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     5461 aaacagctat gaccatgatt acgccaagct tgcatggcgg ccgcataact tcgtatagca
     5521 tacattatac gaagttatgc atgctcggtg ggtcaggtgg aagtggatct ggtgctatga
     5581 tgggcagtgt cgagctgaat ctgagggaga ctgagctgtg tcttggtctt cccggtggag
     5641 atacagtggc tccggtaacc ggaaacaaga gagggttctc agagacggtt gatctgaagc
     5701 taaatctgaa taatgagcct gcaaacaagg aaggatctac gactcatgac gtcgtgactt
     5761 ttgattccaa ggagaagagt gcttgtccta aagatccagc caaacctccg gccaaggcac
     5821 aagttgtggg atggccaccg gtgagatcat accggaagaa cgtgatggtt tcctgccaaa
     5881 aatcaagcgg tggcccggag gcggcggcgt tcgtgaaggt atcaatggac ggagcaccgt
     5941 acttgaggaa aatcgatttg aggatgtata aaagctacga tgagctttct aatgctttgt
     6001 ccaacatgtt cagctctttt accatgggca aacatggagg agaagaagga atgatagact
     6061 tcatgaatga gaggaaattg atggatttgg tgaatagctg ggactatgtt ccctcttatg
     6121 aagacaaaga cggtgattgg atgctcgtcg gcgacgttcc ttggccaatg ttcgtcgata
     6181 catgcaagcg tttacgtctc atgaaaggat cggatgccat tggtctcgct ccgagggcga
     6241 tggagaagtg caagagcaga gctggtgctg gagcaggcgc ctacccatac gatgttcctg
     6301 actatgcggg ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc
     6361 cagattacgc tgctcagtgc tgaggcgcgc cgctagcact acgccatatc atgcccatcc
     6421 cgctacttct gctgctaatt aaactttaaa catctttcca gcatttactt gaacatcaaa
     6481 ctctaatatc gaaatctttt caaacactta tcacatactc aaacttaaac caacattaaa
     6541 cctgttagac aaagccccgg ggcttgccca tctgttttag acgtcacaga aggaccatgt
     6601 ttattttaaa tttcacatac atgtgtttaa ttaccttcat tgtataaatt ctcgaaactt
     6661 tctccaattc ttcggtcaga taaaaaattt ctctagtcac cgtcttttat agagcttttg
     6721 tcagatgttt ttctggatgg aatcattgtc cagttactat gctttttatt tataagtttg
     6781 aaagtttagt taactttttg ttttctttaa tacatttttt aatcatgtca acatttggga
     6841 tttgtagaag tagtaaattt tttgtacatg ttgtagaatt ggataagaac gaaaacagct
     6901 agtatgtaaa aaaaacgaaa atcccaaatt ccgttgtttg tttgatatag taatatttat
     6961 agcattttag tttctaaaga tttttgataa tcttttacat ttgctttcac atacattcgc
     7021 gctactacca tacatcatat cccgctttct ttaattgctt ccccacagac tcaaccagcg
     7081 gggccttctt ctcgagatct gtttagcttg cctcgtcccc gccgggtcac ccggccagcg
     7141 acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat
     7201 gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac
     7261 attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc
     7321 agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa
     7381 atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct
     7441 tgctaggata cagttctcac atcacatccg aacataaaca accatgggta aggaaaagac
     7501 tcacgtttcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     7561 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga
     7621 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     7681 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     7741 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggcaaaa cagcattcca
     7801 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     7861 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     7921 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     7981 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt
     8041 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     8101 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     8161 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     8221 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     8281 tgagtttttc taatcagtac tgacaataaa aagattcttg ttttcaagaa cttgtcattt
     8341 gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt
     8401 ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca
     8461 tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata ctaacgccgc
     8521 catccagtcg acgaactagt ataacttcgt atatggtatt atatacgaag ttatgcggcc
     8581 gcgcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8641 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8701 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     8761 attttctcct tacgcatctg tgcggtattt cacaccgcat accatcgtcc ggatcaatga
     8821 agtagatgag tttactacct gtatatactt ttttttttgt caaagtcact tttatggcat
     8881 ttccatgttg aaggattagc cacagcacta aattttcagc gtatgattgc ttttaaatat
     8941 ttaattttca tcgtttttta attaatattc aaaacgattt aatgccttct acatctgaag
     9001 tcaagctcat agactcgggt tgggtagcta aaactacatc taacacgaag gggtatagcc
     9061 ttcttaaagt atactcatgt ataaacgttt tgggttcata tttttgggcc acgtggttta
     9121 taaaaattct taactacacc actcggtatc ccgcacccgt ctacgtttct acgatttcga
     9181 gctaatattg agtaaagtga ttagcaaaaa aaataaaagt actcgttgtc ggagatcaag
     9241 aatttttcta ttatctcatc taaaccactt tctaaaagcg aaaaacaaaa tcgtaatatg
     9301 cagcttgaat gggcttccat agtttgaaag aaaaacccta gcagtactgg caagggagac
     9361 attcctttta cctggctaat cgccatcttc cagcaggcgc accattgccc ctgtttcact
     9421 atccaggtta cggatatagt tcatgacaat atttacattg gtccagccac cagcttgcat
     9481 gatctccggt attgaaactc cagcgcgggc catatctcgc gcggctccga cacgggcact
     9541 gtgtccagac caggccaggt atctctgacc agagtcatcc ttagcgccgt aaatcaatcg
     9601 atgagttgct tcaaaaatcc cttccagggc gcgagttgat agctggctgg tggcagatgg
     9661 cgcggcaaca ccattttttc tgacccggca aaacaggtag ttattcggat catcagctac
     9721 accagagacg gaaatccatc gctcgaccag tttagttacc cccaggctaa gtgccttctc
     9781 tacacctgcg gtgctaacca gcgttttcgt tctgccaata tggattaaca ttctcccacc
     9841 gtcagtacgt gagatatctt taaccctgat cctggcaatt tcggctatac gtaacagggt
     9901 gttataagca atccccagaa atgccagatt acgtatatcc tggcagcgat cgctattttc
     9961 catgagtgaa cgaacctggt cgaaatcagt gcgttcgaac gctagagcct gttttgcacg
    10021 ttcaccggca tcaacgtttt cttttcggat ccgccgcata accagtgaaa cagcattgct
    10081 gtcacttggt cgtggcagcc cggaccgacg atgaagcatg tttagctggc ccaaatgttg
    10141 ctggatagtt tttactgcca gaccgcgcgc ctgaagatat agaagataat cgcgaacatc
    10201 ttcaggttct gcgggaaacc atttccggtt attcaacttg caccatgccg cccacgaccg
    10261 gcaaacggac agaagcattt tccaggtatg ctcagaaaac gcctggcgat ccctgaacat
    10321 gtccatcagg ttcttgcgaa cctcatcact cgttgcatcg accggtaact tttagattaa
    10381 catttgttta aatatgaaat taggcaatgt aacatactgc aggcaaattt tggtgtacgg
    10441 taagtaaatt ggacatatga tttaacaaag cgactataag tcagaaagtg agaatgagat
    10501 tgaaataatt aattcaactt attcaattga tgatatgcca ggattcctct tcctttatct
    10561 tcaatgtttc gattaacagg tccattatcc ggtttttagt ttcgctgcac attgccgaat
    10621 gacaatcgga ttcctttaag ctttgctttt gccctgtttt ctctccccag taatcgttgg
    10681 gtgactgaac catccagtag ccgagagtta cgtttttcct gatttaataa atatccatat
    10741 ctatttgtct cgtggtgttt tacagcaact aaccgaaatt actattttgc atcatccaac
    10801 cataaagaga caacaataat tctagaaaac atataacaag tgtttttttc tatttacgtc
    10861 tcatacgcaa agtccacttt accatgtttt ggaaacttgt acccaaccat tcatttttcg
    10921 attttgtaat gaggctcttt tcttctaaca atcattgagg tcattatcat gtgtcctcac
    10981 ctattgttta aattgaggct tgcgctattc ccaaaattgt gggtattatt ttcatatatc
    11041 agtatctgca atttcggttc aactccggaa gctcggtacc caactgtggg aatactcagg
    11101 tatcgtaaga tgcaagagtt cgaatctctt agcaaccatt attttttttc tctcaacata
    11161 acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga aattttttgt
    11221 ta
//
