LOCUS       sequence-332982-       10384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA and EGFP tagged RPL22, neomycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Gregory JA, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, 
            Barretto N, Schrode N, Akbalik G, Kang K, Cheng E, Bowles K, Lotz S,
            Goderie S, Karch CM, Temple S, Goate A, Brennand KJ, Phatnani H
  TITLE     Cell Type-Specific In Vitro Gene Expression Profiling of Stem 
            Cell-Derived Neural Models.
  JOURNAL   Cells. 2020 Jun 5;9(6). pii: cells9061406. doi: 
            10.3390/cells9061406.
  PUBMED    32516938
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-06-5"; volume: "9(6). pii: cells9061406. doi"; pages: " 
            10.3390/cells9061406"
COMMENT     Sequence Label: P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo
FEATURES             Location/Qualifiers
     source          1..10384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..27
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(144..161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(315..903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(395..414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1074..1865,1866..1934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1697..1716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1935..2039)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2114..2133)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2305..2684
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2686..2884
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2839..2859
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2902..3082
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3129..3254
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3747..3980
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4165..4209
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4507..4624
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4718..4929
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      4948..4957
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5353..5433
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5449..5505
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5518..6228
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             5518..6228
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(5557..5578)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5818..5837)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6165..6186
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6238..6291
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6304..7104
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6364..6383)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6974..6993
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7143..7731
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7196..7216)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7614..7625)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7734..7750)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7735..7751)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8256..8436
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8462..8479)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8468..8692
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8738..9166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8825..8844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9035..9056
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9175..9195)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9180..9509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9360..9495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9422..9441
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9557..9604
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9623..9997
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10127..10248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10164..10183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10218..10237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10297..10313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10297..10313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10310..10332)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10321..10337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10345..10351,10352..10369,10370..10375))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
       61 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      121 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      181 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      241 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      301 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      721 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
      841 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      901 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1201 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2041 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     2101 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     2161 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     2221 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     2281 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     2341 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2401 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2461 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2521 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2581 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2641 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2701 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2761 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2821 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2881 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     2941 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3001 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3061 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     3121 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     3181 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     3241 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     3301 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     3361 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     3421 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     3481 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     3541 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     3601 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     3661 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     3721 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     3781 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     3841 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     3901 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     3961 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     4021 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     4081 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     4141 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     4201 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     4261 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     4321 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     4381 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     4441 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     4501 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     4561 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     4621 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaagc tagctaggtc
     4681 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4741 gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc
     4801 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     4861 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     4921 agaacacagg accggttaat ctgcgctgcc accatggctc ctgtgaaaaa gcttgtggtg
     4981 aaggggggca aaaaaaagaa gcaagttctg aagttcactc ttgattgcac ccaccctgta
     5041 gaagatggaa tcatggatgc tgccaatttt gagcagtttt tgcaagaaag gatcaaagtg
     5101 aacggaaaag ctgggaacct tggtggaggg gtggtgacca tcgaaaggag caagagcaag
     5161 atcaccgtga catccgaggt gcctttctcc aaaaggtatt tgaaatatct caccaaaaaa
     5221 tatttgaaga agaataatct acgtgactgg ttgcgcgtag ttgctaacag caaagagagt
     5281 tacgaattac gttacttcca gattaaccag gacgaagaag aggaggaaga cgaggatgga
     5341 ggtggatcag gatacccata cgatgttcca gattacgctt atccatatga tgtcccagac
     5401 tatgcttacc cttacgacgt tcctgactac gccgaattcg gatctggcgc cacgaatttc
     5461 tcgctactca agcaggccgg tgatgtcgag gaaaaccctg gtcctatggt gtctagaagc
     5521 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     5581 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     5641 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     5701 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     5761 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     5821 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     5881 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     5941 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     6001 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     6061 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     6121 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     6181 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg ttcaggggag
     6241 ggaagaggtt ccctgctcac atgcggagac gtagaagaga atcccggacc catgacctct
     6301 agaggatcgg ctattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     6361 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     6421 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     6481 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     6541 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6601 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     6661 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6721 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6781 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     6841 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6901 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6961 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     7021 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     7081 cgccttcttg acgagttctt ctgattaatt aagctagcta attcgatatc aagcttatcg
     7141 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7201 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7261 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7321 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7381 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7441 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7501 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7561 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7621 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7681 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     7741 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     7801 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     7861 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7921 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     7981 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     8041 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     8101 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     8161 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     8221 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     8281 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8341 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8401 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     8461 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     8521 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     8581 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     8641 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     8701 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     8761 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8821 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8881 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8941 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9001 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9061 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9121 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     9181 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9241 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9301 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9361 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9421 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     9481 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     9541 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     9601 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     9661 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     9721 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     9781 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     9841 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     9901 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     9961 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
    10021 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
    10081 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
    10141 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10201 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    10261 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10321 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10381 gggt
//
