LOCUS       sequence-332983-        9436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 and mCherry tagged RPL22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Gregory JA, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, 
            Barretto N, Schrode N, Akbalik G, Kang K, Cheng E, Bowles K, Lotz S,
            Goderie S, Karch CM, Temple S, Goate A, Brennand KJ, Phatnani H
  TITLE     Cell Type-Specific In Vitro Gene Expression Profiling of Stem 
            Cell-Derived Neural Models.
  JOURNAL   Cells. 2020 Jun 5;9(6). pii: cells9061406. doi: 
            10.3390/cells9061406.
  PUBMED    32516938
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-06-5"; volume: "9(6). pii: cells9061406. doi"; pages: " 
            10.3390/cells9061406"
COMMENT     Sequence Label: P-Ef1a-mCherry-T2A-RPL22-1xV5
FEATURES             Location/Qualifiers
     source          1..9436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(108..128)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        113..442
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      293..428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     355..374
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        490..537
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             556..930
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1060..1181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1097..1116)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1151..1170
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1230..1246)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1230..1246)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1243..1265)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1254..1270
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1278..1284,1285..1302,1303..1308))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1323..1344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1461..1478)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1632..2220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1712..1731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2391..3182,3183..3251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3014..3033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3252..3356)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3431..3450)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3622..4001
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4003..4201
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4156..4176
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4219..4399
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4446..4571
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5064..5297
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5482..5526
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5824..5941
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6035..6246
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6267..6276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6273..6980
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6418..6436)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6686..6705
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6990..7043
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7443..7484
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7512..8100
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7565..7585)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7983..7994)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8103..8119)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8104..8120)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8625..8805
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8831..8848)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8837..9061
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9107..9436,1..99)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9194..9213)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9404..9425
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
       61 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
      121 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      181 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      241 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
      301 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
      361 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
      421 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
      481 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
      541 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
      601 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
      661 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
      721 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
      781 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
      841 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
      901 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
      961 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     1021 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     1081 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1141 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     1201 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     1261 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     1321 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1381 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1441 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1501 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1561 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1621 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1681 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1741 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1801 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1861 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1921 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1981 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2041 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2101 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2161 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2221 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2281 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2341 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2401 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2461 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2521 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2581 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2641 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2701 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2761 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2821 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2881 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2941 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3001 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3061 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3361 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     3421 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     3481 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     3541 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     3601 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     3661 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     3721 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     3781 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     3841 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     3901 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     3961 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     4021 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     4081 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     4141 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     4201 gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4261 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4321 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4381 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     4441 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     4501 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     4561 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     4621 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     4681 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     4741 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     4801 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     4861 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     4921 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     4981 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     5041 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     5101 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     5161 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     5221 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     5281 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     5341 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     5401 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     5461 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     5521 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     5581 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     5641 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     5701 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     5761 ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca
     5821 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     5881 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     5941 tcgggtttat tacagggaca gcagagatcc agtttggtta attaagctag ctaggtcttg
     6001 aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     6061 cccgagaagt tggggggagg ggtcggcaat tgatccggtg cctagagaag gtggcgcggg
     6121 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     6181 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     6241 acacaggacc ggtgccggcc ggatctgcca ccatggtgag caagggcgag gaggataaca
     6301 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     6361 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     6421 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     6481 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     6541 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     6601 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     6661 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     6721 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     6781 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     6841 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     6901 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     6961 gcatggacga gctgtacaag ggttcagggg agggaagagg ttccctgctc acatgcggag
     7021 acgtagaaga gaatcccgga cccatggctc ctgtgaaaaa gcttgtggtg aaggggggca
     7081 aaaaaaagaa gcaagttctg aagttcactc ttgattgcac ccaccctgta gaagatggaa
     7141 tcatggatgc tgccaatttt gagcagtttt tgcaagaaag gatcaaagtg aacggaaaag
     7201 ctgggaacct tggtggaggg gtggtgacca tcgaaaggag caagagcaag atcaccgtga
     7261 catccgaggt gcctttctcc aaaaggtatt tgaaatatct caccaaaaaa tatttgaaga
     7321 agaataatct acgtgactgg ttgcgcgtag ttgctaacag caaagagagt tacgaattac
     7381 gttacttcca gattaaccag gacgaagaag aggaggaaga cgaggatgga ggtggatcag
     7441 gaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtaagaa ttcgatatca
     7501 agcttatcgg taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7561 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7621 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7681 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7741 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7801 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7861 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7921 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7981 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8041 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8101 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     8161 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     8221 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8281 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     8341 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     8401 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     8461 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     8521 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     8581 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     8641 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8701 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8761 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     8821 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     8881 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     8941 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     9001 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     9061 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     9121 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     9181 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9241 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     9301 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     9361 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     9421 actggaacaa cactca
//
