LOCUS       sequence-33299-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of humanized ChR2(H134R) fused to EYFP, for 
            optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  TITLE     hChR2 plasmids
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-GFAP-hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttggagagg agacgcatca cctccgctgc tcgccgggat cctctagagt cgactccgga
       61 gccaccatgg actatggcgg cgctttgtct gccgtcggac gcgaactttt gttcgttact
      121 aatcctgtgg tggtgaacgg gtccgtcctg gtccctgagg atcaatgtta ctgtgccgga
      181 tggattgaat ctcgcggcac gaacggcgct cagaccgcgt caaatgtcct gcagtggctt
      241 gcagcaggat tcagcatttt gctgctgatg ttctatgcct accaaacctg gaaatctaca
      301 tgcggctggg aggagatcta tgtgtgcgcc attgaaatgg ttaaggtgat tctcgagttc
      361 ttttttgagt ttaagaatcc ctctatgctc taccttgcca caggacaccg ggtgcagtgg
      421 ctgcgctatg cagagtggct gctcacttgt cctgtcatcc ttatccgcct gagcaacctc
      481 accggcctga gcaacgacta cagcaggaga accatgggac tccttgtctc agacatcggg
      541 actatcgtgt ggggggctac cagcgccatg gcaaccggct atgttaaagt catcttcttt
      601 tgtcttggat tgtgctatgg cgcgaacaca ttttttcacg ccgccaaagc atatatcgag
      661 ggttatcata ctgtgccaaa gggtcggtgc cgccaggtcg tgaccggcat ggcatggctg
      721 tttttcgtga gctggggtat gttcccaatt ctcttcattt tggggcccga aggttttggc
      781 gtcctgagcg tctatggctc caccgtaggt cacacgatta ttgatctgat gagtaaaaat
      841 tgttgggggt tgttgggaca ctacctgcgc gtcctgat
//
