LOCUS       sequence-333001-       11386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Double-floxed inverse Orientation HA and IRES-YFP tagged RPL22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Gregory JA, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, 
            Barretto N, Schrode N, Akbalik G, Kang K, Cheng E, Bowles K, Lotz S,
            Goderie S, Karch CM, Temple S, Goate A, Brennand KJ, Phatnani H
  TITLE     Cell Type-Specific In Vitro Gene Expression Profiling of Stem 
            Cell-Derived Neural Models.
  JOURNAL   Cells. 2020 Jun 5;9(6). pii: cells9061406. doi: 
            10.3390/cells9061406.
  PUBMED    32516938
REFERENCE   2  (bases 1 to 11386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-06-5"; volume: "9(6). pii: cells9061406. doi"; pages: " 
            10.3390/cells9061406"
COMMENT     Sequence Label: Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP
FEATURES             Location/Qualifiers
     source          1..11386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2463..3641
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2694..3632
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3589..3609
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(3672..3705)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3756..3789)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(3798..4511,4512..4514,4515..4517))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3843..3864)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4192..4211
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4451..4472
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(4518..4526,4527..4529,4530..5104)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4691..4710)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4920..4937
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     misc_feature    5106..5158
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(5168..5257)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     regulatory      5644..5653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    5660..5693
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5744..5777
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5841..6429
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5894..5914)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6312..6323)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6432..6448)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6433..6449)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6954..7134
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7160..7177)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7166..7390
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7436..7864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7523..7542)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7733..7754
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7873..7893)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7878..8207
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8058..8193
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8120..8139
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8255..8302
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8321..8695
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8825..8946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8862..8881)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8916..8935
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8995..9011)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8995..9011)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9008..9030)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9019..9035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9043..9049,9050..9067,9068..9073))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9088..9109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9226..9243)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9397..9985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9477..9496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10156..10947,10948..11016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10779..10798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11017..11121)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11196..11215)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaagctagt acgcgtaagc tttgcaaaga tggataaagt
     2401 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
     2461 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2521 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2581 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2641 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2701 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2761 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2821 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2881 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2941 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     3001 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     3061 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     3121 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     3181 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3241 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3301 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3361 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3421 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3481 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3541 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3601 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggtaccgga tcctctagag
     3661 tcgactccgg aataacttcg tataggatac tttatacgaa gttatgcaga atggtagctg
     3721 gattgtagct gctattagca atatgaaacc tcttaataac ttcgtatagc atacattata
     3781 cgaagttatg gcgcgcctta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
     3841 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
     3901 gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
     3961 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
     4021 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
     4081 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
     4141 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
     4201 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
     4261 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag
     4321 ccgaaggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
     4381 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
     4441 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
     4501 tcgcccttgc tcaccatggt tgtggccata ttatcatcgt gtttttcaaa ggaaaaccac
     4561 gtccccgtgg ttcggggggc ctagacgttt ttttaacctc gactaaacac atgtaaagca
     4621 tgtgtaccga ggccccagat cagatcccat acaatggggt accttctggg catccttcag
     4681 ccccttgttg aatacgcttg aggagagcca tttgactctt tccacaacta tccaactcac
     4741 aacgtggcac tggggttgtg ccgcctttgc aggtgtatct tatacacgtg gcttttggcc
     4801 gcagaggcac ctgtcgccag gtggggggtt ccgctgcctg caaagggtcg ctacagacgt
     4861 tgtttgtctt caagaagctt ccagaggaac tgcttccttc acgacattca acagaccttg
     4921 cattcctttg gcgagagggg aaagacccct aggaatgctc gtcaagaaga cagggccagg
     4981 tttccgggcc ctcacattgc caaaagacgg caatatggtg gaaaataaca tatagacaaa
     5041 cgcacaccgg ccttattcca agcggcttcg gccagtaacg ttaggggggg gggagggaga
     5101 ggggcggatc ccgggcccgc ggtaccgtcg actgcagaat tcgaagcttg agctcgagct
     5161 actgagcagc gtaatctgga acgtcatatg gataggatcc tgcatagtcc gggacgtcat
     5221 agggatagcc cgcatagtca ggaacatcgt atgggtagaa ttcatcctcg tcttcctcct
     5281 cctcttcatc ctggttaatc tggaagtaac gcagctcgta actctctttg ctgttggcga
     5341 caacacgcag ccagtctcgg aggttgttct tcttcaaata ttttttggtg agatatttca
     5401 aatacctttt ggagaaaggc acctctgaag tgacagtgat cttgctcttg ctgcgttcga
     5461 tggtcacgac tcctccgccg aggttgccag ctttcccatt caccttgatt ctctcctgga
     5521 ggaactgctc aaaattggca gcatccatga ttccatcttc tacagggtga gtgcagtcca
     5581 gagtgaactt caaaacctgc ttcttttttt tgcccccctt cgccacaagc tttttcacag
     5641 gcgccatggt ggcgctagca taacttcgta taaagtatcc tatacgaagt tatttgcctt
     5701 aacccagaaa ttatcactgt tattctttag aatggtgcaa agaataactt cgtataatgt
     5761 atgctatacg aagttatgaa ttcgatatca agcttatcga taatcaacct actagctaat
     5821 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     5881 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5941 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6001 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6061 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6121 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6181 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6241 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6301 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6361 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6421 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     6481 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     6541 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     6601 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6661 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     6721 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     6781 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     6841 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     6901 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     6961 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7021 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7081 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     7141 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7201 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7261 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     7321 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     7381 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     7441 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7501 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7561 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7621 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7681 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7741 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7801 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7861 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7921 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7981 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     8041 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8101 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     8161 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     8221 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     8281 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     8341 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     8401 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     8461 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     8521 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     8581 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     8641 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     8701 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8761 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8821 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8881 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8941 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     9001 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9061 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9121 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9181 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10141 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10201 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10261 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10321 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10381 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10441 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10501 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10561 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10621 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10681 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10741 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10801 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10861 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10921 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10981 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11041 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11101 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    11161 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    11221 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    11281 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    11341 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
