LOCUS       sequence-333005-        9536 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tetracycline-inducible expression of HA and EGFP tagged RPL22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Gregory JA, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, 
            Barretto N, Schrode N, Akbalik G, Kang K, Cheng E, Bowles K, Lotz S,
            Goderie S, Karch CM, Temple S, Goate A, Brennand KJ, Phatnani H
  TITLE     Cell Type-Specific In Vitro Gene Expression Profiling of Stem 
            Cell-Derived Neural Models.
  JOURNAL   Cells. 2020 Jun 5;9(6). pii: cells9061406. doi: 
            10.3390/cells9061406.
  PUBMED    32516938
REFERENCE   2  (bases 1 to 9536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-06-5"; volume: "9(6). pii: cells9061406. doi"; pages: " 
            10.3390/cells9061406"
COMMENT     Sequence Label: P-rTetO-EGFP-T2A-RPL22-3xHA
FEATURES             Location/Qualifiers
     source          1..9536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    290..407
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    464..482
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    506..524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    548..566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    590..608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        641..679
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     676..700
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      779..788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(785..787,788..790,791..1501)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(785..787,788..790,791..1501)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(830..851)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1091..1110)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1438..1459
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1511..1564
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1964..2044
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    2078..2666
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2131..2151)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2549..2560)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2669..2685)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2670..2686)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3191..3371
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3397..3414)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3403..3627
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3673..4101
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3760..3779)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3970..3991
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4110..4130)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4115..4444
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4295..4430
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4357..4376
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4492..4539
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4558..4932
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5062..5183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5099..5118)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5153..5172
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5232..5248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5232..5248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5245..5267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5256..5272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5280..5286,5287..5304,5305..5310))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5325..5346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5463..5480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5634..6222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5714..5733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6393..7184,7185..7253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7016..7035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7254..7358)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7433..7452)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7624..8003
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8005..8203
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8221..8401
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8448..8573
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9066..9299
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9484..9528
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
       61 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
      121 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
      181 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
      241 ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa
      301 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
      361 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
      421 gggacagcag agatccagtt tggttagatc tcgagtttac cactccctat cagtgataga
      481 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      541 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      601 gatagagaaa agtgaaagtc gagctcggta cccgggtcga ggtaggcgtg tacggtggga
      661 ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
      721 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaattcgc
      781 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      841 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      901 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      961 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     1021 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1081 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1141 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1201 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1261 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1321 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1381 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1441 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1501 gggttcaggg gagggaagag gttccctgct cacatgcgga gacgtagaag agaatcccgg
     1561 acccatggct cctgtgaaaa agcttgtggt gaaggggggc aaaaaaaaga agcaagttct
     1621 gaagttcact cttgattgca cccaccctgt agaagatgga atcatggatg ctgccaattt
     1681 tgagcagttt ttgcaagaaa ggatcaaagt gaacggaaaa gctgggaacc ttggtggagg
     1741 ggtggtgacc atcgaaagga gcaagagcaa gatcaccgtg acatccgagg tgcctttctc
     1801 caaaaggtat ttgaaatatc tcaccaaaaa atatttgaag aagaataatc tacgtgactg
     1861 gttgcgcgta gttgctaaca gcaaagagag ttacgaatta cgttacttcc agattaacca
     1921 ggacgaagaa gaggaggaag acgaggatgg aggtggatca ggatacccat acgatgttcc
     1981 agattacgct tatccatatg atgtcccaga ctatgcttac ccttacgacg ttcctgacta
     2041 cgcctaagct agctaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     2101 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     2161 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     2221 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     2281 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     2341 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     2401 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     2461 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     2521 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     2581 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     2641 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     2701 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     2761 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     2821 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     2881 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     2941 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     3001 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     3061 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     3121 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     3181 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     3241 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3301 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3361 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     3421 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3481 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3541 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     3601 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     3661 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3721 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3781 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3841 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3901 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3961 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4021 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4081 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4141 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4201 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4261 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4321 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4381 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4441 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     4501 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     4561 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     4621 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     4681 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     4741 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     4801 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     4861 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     4921 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4981 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     5041 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     5101 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5161 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     5221 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5281 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5401 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6781 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7381 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     7441 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     7501 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     7561 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     7621 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     7681 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     7741 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     7801 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     7861 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     7921 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     7981 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     8041 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     8101 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     8161 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
     8221 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8281 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8341 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8401 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     8461 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     8521 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     8581 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     8641 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     8701 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     8761 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     8821 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     8881 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     8941 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     9001 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     9061 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     9121 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     9181 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     9241 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     9301 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     9361 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     9421 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     9481 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattg
//
