LOCUS       sequence-333010-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG and mCherry tagged RPL22, puromycin resistance .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Gregory JA, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, 
            Barretto N, Schrode N, Akbalik G, Kang K, Cheng E, Bowles K, Lotz S,
            Goderie S, Karch CM, Temple S, Goate A, Brennand KJ, Phatnani H
  TITLE     Cell Type-Specific In Vitro Gene Expression Profiling of Stem 
            Cell-Derived Neural Models.
  JOURNAL   Cells. 2020 Jun 5;9(6). pii: cells9061406. doi: 
            10.3390/cells9061406.
  PUBMED    32516938
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-06-5"; volume: "9(6). pii: cells9061406. doi"; pages: " 
            10.3390/cells9061406"
COMMENT     Sequence Label: P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..118)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        103..432
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      283..418
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     345..364
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        480..527
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             546..920
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1050..1171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1087..1106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1141..1160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1220..1236)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1220..1236)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1233..1255)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1244..1260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1268..1274,1275..1292,1293..1298))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1313..1334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1451..1468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1622..2210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1702..1721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2381..3172,3173..3241))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3004..3023
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3242..3346)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3421..3440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3612..3991
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3993..4191
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4146..4166
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4209..4389
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4436..4561
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5054..5287
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5472..5516
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5814..5931
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6025..6236
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6255..6264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6660..6725
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6741..6797
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6810..7514
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6952..6970)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7220..7239
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7524..7577
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7590..8183
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="EYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVT
                     ELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQ
                     QQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAP
                     RNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8080..8100
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8222..8810
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8275..8295)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8693..8704)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8813..8829)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8814..8830)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9335..9515
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9541..9558)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9547..9771
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9817..10156,1..89)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9904..9923)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10114..10135
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
       61 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
      121 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
      181 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      241 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
      301 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
      361 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
      421 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
      481 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
      541 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
      601 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
      661 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
      721 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
      781 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
      841 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
      901 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
      961 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     1021 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     1081 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     1141 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     1201 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1261 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1321 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1381 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1441 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1501 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1561 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1621 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1681 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1741 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1801 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1861 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1921 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1981 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2041 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2101 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2161 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2221 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2281 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2341 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2401 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2461 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2521 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2581 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2641 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2701 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2761 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2821 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2881 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2941 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3001 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3061 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3121 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3181 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3241 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3301 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3361 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     3421 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     3481 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     3541 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     3601 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     3661 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     3721 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     3781 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     3841 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     3901 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     3961 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     4021 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     4081 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     4141 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
     4201 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4261 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4321 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4381 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     4441 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     4501 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     4561 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     4621 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     4681 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     4741 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     4801 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     4861 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     4921 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     4981 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     5041 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     5101 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     5161 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     5221 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     5281 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     5341 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     5401 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     5461 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     5521 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     5581 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     5641 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     5701 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     5761 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     5821 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     5881 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     5941 tacagggaca gcagagatcc agtttggtta attaagctag ctaggtcttg aaaggagtgg
     6001 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     6061 tggggggagg ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg
     6121 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     6181 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc
     6241 ggttaatctg cgctgccacc atggctcctg tgaaaaagct tgtggtgaag gggggcaaaa
     6301 aaaagaagca agttctgaag ttcactcttg attgcaccca ccctgtagaa gatggaatca
     6361 tggatgctgc caattttgag cagtttttgc aagaaaggat caaagtgaac ggaaaagctg
     6421 ggaaccttgg tggaggggtg gtgaccatcg aaaggagcaa gagcaagatc accgtgacat
     6481 ccgaggtgcc tttctccaaa aggtatttga aatatctcac caaaaaatat ttgaagaaga
     6541 ataatctacg tgactggttg cgcgtagttg ctaacagcaa agagagttac gaattacgtt
     6601 acttccagat taaccaggac gaagaagagg aggaagacga ggatggaggt ggatcaggag
     6661 actacaagga tcacgacggt gattacaagg accacgatat cgactacaag gatgacgatg
     6721 acaaggaatt cggatctggc gccacgaatt tctcgctact caagcaggcc ggtgatgtcg
     6781 aggaaaaccc tggtcctatg gtgtctagag tgagcaaggg cgaggaggat aacatggcca
     6841 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     6901 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     6961 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     7021 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     7081 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     7141 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     7201 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     7261 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     7321 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     7381 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     7441 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     7501 acgagctgta caagggttca ggggagggaa gaggttccct gctcacatgc ggagacgtag
     7561 aagagaatcc cggacccatg acctctagag agtacaagcc cacggtgcgc ctcgccaccc
     7621 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     7681 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     7741 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     7801 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     7861 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     7921 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     7981 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     8041 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     8101 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     8161 tgacccgcaa gcccggtgcc tgattaatta agctagctaa ttcgatatca agcttatcga
     8221 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     8281 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     8341 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     8401 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     8461 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     8521 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     8581 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     8641 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     8701 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8761 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     8821 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     8881 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     8941 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     9001 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     9061 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     9121 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     9181 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     9241 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     9301 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     9361 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9421 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9481 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     9541 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     9601 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     9661 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     9721 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     9781 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     9841 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     9901 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     9961 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
    10021 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
    10081 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
    10141 cactcaaccc tatctc
//
