LOCUS       sequence-333011-        9427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG and EGFP tagged RPL22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Gregory JA, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, 
            Barretto N, Schrode N, Akbalik G, Kang K, Cheng E, Bowles K, Lotz S,
            Goderie S, Karch CM, Temple S, Goate A, Brennand KJ, Phatnani H
  TITLE     Cell Type-Specific In Vitro Gene Expression Profiling of Stem 
            Cell-Derived Neural Models.
  JOURNAL   Cells. 2020 Jun 5;9(6). pii: cells9061406. doi: 
            10.3390/cells9061406.
  PUBMED    32516938
REFERENCE   2  (bases 1 to 9427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-06-5"; volume: "9(6). pii: cells9061406. doi"; pages: " 
            10.3390/cells9061406"
COMMENT     Sequence Label: P-Ef1a-EGFP-T2A-RPL22-1xFlag
FEATURES             Location/Qualifiers
     source          1..9427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        54..101
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             120..494
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    624..745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(661..680)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     715..734
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(794..810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(794..810)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(807..829)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    818..834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(842..848,849..866,867..872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    887..908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1025..1042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1196..1784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1276..1295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1955..2746,2747..2815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2578..2597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2816..2920)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2995..3014)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3186..3565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3567..3765
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3720..3740
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3783..3963
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4010..4135
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4628..4861
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5046..5090
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5388..5505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5599..5810
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      5831..5840
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5837..5839,5840..5842,5843..6553)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5837..5839,5840..5842,5843..6553)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5882..5903)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6143..6162)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6490..6511
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6563..6616
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7016..7039
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7067..7655
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7120..7140)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7538..7549)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7658..7674)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7659..7675)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8180..8360
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8386..8403)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8392..8616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8662..9090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8749..8768)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8959..8980
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9099..9119)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9104..9427,1..6)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9284..9419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9346..9365
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
       61 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
      121 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
      181 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
      241 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
      301 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
      361 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
      421 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
      481 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
      541 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
      601 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
      661 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
      721 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
      781 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      841 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      901 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      961 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     1021 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1081 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1141 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1201 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1261 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1321 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1381 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1441 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1501 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1561 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1621 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1681 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1741 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1801 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1861 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1921 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1981 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2041 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2101 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2161 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2221 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2281 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2341 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2401 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2461 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     2521 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     2581 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     2641 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     2701 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     2761 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     2821 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2881 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2941 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     3001 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     3061 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     3121 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     3181 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     3241 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     3301 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     3361 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     3421 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     3481 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     3541 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3601 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     3661 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     3721 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
     3781 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3841 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3901 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3961 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     4021 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     4081 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     4141 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     4201 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     4261 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     4321 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     4381 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     4441 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     4501 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     4561 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     4621 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     4681 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     4741 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     4801 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     4861 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     4921 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     4981 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     5041 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     5101 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     5161 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     5221 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     5281 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     5341 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     5401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     5461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     5521 gacagcagag atccagtttg gttaattaag ctagctaggt cttgaaagga gtgggaattg
     5581 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     5641 gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     5701 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     5761 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gaccggtgcc
     5821 ggccggatct gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     5881 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     5941 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     6001 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     6061 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     6121 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     6181 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     6241 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     6301 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     6361 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     6421 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     6481 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     6541 cgagctgtac aagggttcag gggagggaag aggttccctg ctcacatgcg gagacgtaga
     6601 agagaatccc ggacccatgg ctcctgtgaa aaagcttgtg gtgaaggggg gcaaaaaaaa
     6661 gaagcaagtt ctgaagttca ctcttgattg cacccaccct gtagaagatg gaatcatgga
     6721 tgctgccaat tttgagcagt ttttgcaaga aaggatcaaa gtgaacggaa aagctgggaa
     6781 ccttggtgga ggggtggtga ccatcgaaag gagcaagagc aagatcaccg tgacatccga
     6841 ggtgcctttc tccaaaaggt atttgaaata tctcaccaaa aaatatttga agaagaataa
     6901 tctacgtgac tggttgcgcg tagttgctaa cagcaaagag agttacgaat tacgttactt
     6961 ccagattaac caggacgaag aagaggagga agacgaggat ggaggtggat caggagacta
     7021 caaggatgac gatgacaagt aagaattcga tatcaagctt atcggtaatc aacctctgga
     7081 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7141 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7201 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7261 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7321 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7381 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7441 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     7501 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7561 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7621 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct
     7681 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     7741 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     7801 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7861 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     7921 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     7981 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     8041 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     8101 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     8161 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8221 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8281 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8341 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     8401 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     8461 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8521 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     8581 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     8641 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     8701 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     8761 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     8821 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     8881 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     8941 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9001 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9061 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     9121 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9181 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9241 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9301 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9361 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9421 aggcttt
//
