LOCUS       sequence-333016-        9614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tetracycline-inducible expression of V5 and mCherry tagged RPL22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Gregory JA, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, 
            Barretto N, Schrode N, Akbalik G, Kang K, Cheng E, Bowles K, Lotz S,
            Goderie S, Karch CM, Temple S, Goate A, Brennand KJ, Phatnani H
  TITLE     Cell Type-Specific In Vitro Gene Expression Profiling of Stem 
            Cell-Derived Neural Models.
  JOURNAL   Cells. 2020 Jun 5;9(6). pii: cells9061406. doi: 
            10.3390/cells9061406.
  PUBMED    32516938
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-06-5"; volume: "9(6). pii: cells9061406. doi"; pages: " 
            10.3390/cells9061406"
COMMENT     Sequence Label: P-rTetO-mCherry-T2A-RPL22-1xV5
FEATURES             Location/Qualifiers
     source          1..9614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(2..20,21..43,44..62,63..85,86..104,105..127,128..146,
                     147..169,170..188,189..211,212..230,231..253,254..272)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        305..343
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     340..364
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      443..452
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             449..1156
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(594..612)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     862..881
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1166..1219
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1619..1660
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1694..2282
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1747..1767)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2165..2176)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2285..2301)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2286..2302)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2807..2987
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3013..3030)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3019..3243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3289..3717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3376..3395)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3586..3607
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3726..3746)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3731..4060
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3911..4046
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3973..3992
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4108..4155
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4174..4548
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4678..4799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4715..4734)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4769..4788
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4848..4864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4848..4864)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4861..4883)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4872..4888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4896..4902,4903..4920,4921..4926))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4941..4962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5079..5096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5250..5838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5330..5349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6009..6800,6801..6869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6632..6651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6870..6974)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7049..7068)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7240..7619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7621..7819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7837..8017
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8064..8189
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8682..8915
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9100..9144
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9442..9559
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
       61 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      121 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      181 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      241 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc ggtacccggg
      301 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
      361 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      421 cagcctccgc ggccccgaat tcgccaccat ggtgagcaag ggcgaggagg ataacatggc
      481 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      541 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      601 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      661 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      721 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      781 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
      841 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      901 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
      961 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     1021 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     1081 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     1141 ggacgagctg tacaagggtt caggggaggg aagaggttcc ctgctcacat gcggagacgt
     1201 agaagagaat cccggaccca tggctcctgt gaaaaagctt gtggtgaagg ggggcaaaaa
     1261 aaagaagcaa gttctgaagt tcactcttga ttgcacccac cctgtagaag atggaatcat
     1321 ggatgctgcc aattttgagc agtttttgca agaaaggatc aaagtgaacg gaaaagctgg
     1381 gaaccttggt ggaggggtgg tgaccatcga aaggagcaag agcaagatca ccgtgacatc
     1441 cgaggtgcct ttctccaaaa ggtatttgaa atatctcacc aaaaaatatt tgaagaagaa
     1501 taatctacgt gactggttgc gcgtagttgc taacagcaaa gagagttacg aattacgtta
     1561 cttccagatt aaccaggacg aagaagagga ggaagacgag gatggaggtg gatcaggagg
     1621 taagcctatc cctaaccctc tcctcggtct cgattctacg taagctagct aattcgatat
     1681 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     1741 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     1801 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     1861 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     1921 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     1981 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     2041 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     2101 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     2161 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     2221 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     2281 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     2341 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     2401 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     2461 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     2521 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     2581 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     2641 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     2701 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     2761 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     2821 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2881 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2941 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     3001 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     3061 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     3121 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     3181 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     3241 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     3301 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3361 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3421 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3481 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3541 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3601 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3661 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     3721 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     3781 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3841 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     3901 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3961 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     4021 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     4081 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     4141 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     4201 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     4261 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     4321 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     4381 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     4441 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     4501 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     4561 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4621 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     4681 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4741 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4801 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     4861 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4921 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4981 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5041 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5101 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5161 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5221 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5281 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5341 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5401 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5461 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5521 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5581 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5641 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5701 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5761 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5821 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5881 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5941 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6001 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6061 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6121 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6181 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6241 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6301 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6361 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6421 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6481 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6541 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6601 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6661 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6721 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6781 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6841 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6901 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6961 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     7021 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     7081 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     7141 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     7201 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     7261 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     7321 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     7381 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     7441 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     7501 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     7561 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     7621 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     7681 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     7741 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     7801 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
     7861 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7921 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7981 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     8041 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     8101 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     8161 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     8221 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     8281 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     8341 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     8401 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     8461 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     8521 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     8581 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     8641 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     8701 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     8761 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     8821 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     8881 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     8941 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     9001 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     9061 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     9121 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     9181 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     9241 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     9301 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     9361 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     9421 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     9481 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     9541 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaga
     9601 tctcgagttt acca
//
