LOCUS       sequence-333017-       10171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA and mCherry tagged RPL22, puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Gregory JA, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, 
            Barretto N, Schrode N, Akbalik G, Kang K, Cheng E, Bowles K, Lotz S,
            Goderie S, Karch CM, Temple S, Goate A, Brennand KJ, Phatnani H
  TITLE     Cell Type-Specific In Vitro Gene Expression Profiling of Stem 
            Cell-Derived Neural Models.
  JOURNAL   Cells. 2020 Jun 5;9(6). pii: cells9061406. doi: 
            10.3390/cells9061406.
  PUBMED    32516938
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-06-5"; volume: "9(6). pii: cells9061406. doi"; pages: " 
            10.3390/cells9061406"
COMMENT     Sequence Label: P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..10171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..64)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        49..378
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      229..364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     291..310
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        426..473
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             492..866
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    996..1117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1033..1052)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1087..1106
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1166..1182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1166..1182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1179..1201)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1190..1206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1214..1220,1221..1238,1239..1244))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1259..1280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1397..1414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1568..2156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1648..1667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2327..3118,3119..3187))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2950..2969
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3188..3292)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3367..3386)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3558..3937
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3939..4137
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4092..4112
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4155..4335
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4382..4507
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5000..5233
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5418..5462
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5760..5877
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5971..6182
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6201..6210
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6606..6686
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             6702..6758
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6771..7475
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6913..6931)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7181..7200
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7485..7538
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7551..8144
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="EYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVT
                     ELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQ
                     QQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAP
                     RNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8041..8061
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8183..8771
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8236..8256)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8654..8665)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8774..8790)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8775..8791)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9296..9476
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9502..9519)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9508..9732
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9778..10171,1..35)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9865..9884)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10075..10096
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
       61 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      121 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
      181 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
      241 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
      301 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
      361 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
      421 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
      481 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
      541 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
      601 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
      661 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
      721 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
      781 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
      841 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
      901 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
      961 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     1021 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     1081 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     1141 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1201 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1261 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1321 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1381 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1441 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1501 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1561 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1621 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1681 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1741 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1801 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1861 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1921 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1981 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2041 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2101 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2161 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2221 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2281 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2341 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2401 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2461 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2521 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2581 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2641 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2701 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2761 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2821 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2881 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2941 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3001 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3061 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3301 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     3361 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     3421 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     3481 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     3541 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     3601 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     3661 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     3721 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     3781 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     3841 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     3901 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     3961 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     4021 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     4081 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
     4141 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4201 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4261 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4321 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     4381 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     4441 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     4501 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     4561 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     4621 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     4681 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     4741 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     4801 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     4861 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     4921 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     4981 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     5041 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     5101 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     5161 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     5221 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     5281 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     5341 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     5401 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     5461 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     5521 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     5581 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     5641 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     5701 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     5761 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     5821 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     5881 gtttattaca gggacagcag agatccagtt tggttaatta agctagctag gtcttgaaag
     5941 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     6001 agaagttggg gggaggggtc ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa
     6061 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     6121 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     6181 aggaccggtt aatctgcgct gccaccatgg ctcctgtgaa aaagcttgtg gtgaaggggg
     6241 gcaaaaaaaa gaagcaagtt ctgaagttca ctcttgattg cacccaccct gtagaagatg
     6301 gaatcatgga tgctgccaat tttgagcagt ttttgcaaga aaggatcaaa gtgaacggaa
     6361 aagctgggaa ccttggtgga ggggtggtga ccatcgaaag gagcaagagc aagatcaccg
     6421 tgacatccga ggtgcctttc tccaaaaggt atttgaaata tctcaccaaa aaatatttga
     6481 agaagaataa tctacgtgac tggttgcgcg tagttgctaa cagcaaagag agttacgaat
     6541 tacgttactt ccagattaac caggacgaag aagaggagga agacgaggat ggaggtggat
     6601 caggataccc atacgatgtt ccagattacg cttatccata tgatgtccca gactatgctt
     6661 acccttacga cgttcctgac tacgccgaat tcggatctgg cgccacgaat ttctcgctac
     6721 tcaagcaggc cggtgatgtc gaggaaaacc ctggtcctat ggtgtctaga gtgagcaagg
     6781 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     6841 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     6901 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     6961 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     7021 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     7081 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     7141 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     7201 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     7261 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     7321 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     7381 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     7441 gccactccac cggcggcatg gacgagctgt acaagggttc aggggaggga agaggttccc
     7501 tgctcacatg cggagacgta gaagagaatc ccggacccat gacctctaga gagtacaagc
     7561 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     7621 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     7681 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     7741 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     7801 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     7861 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     7921 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     7981 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     8041 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8101 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgattaatt aagctagcta
     8161 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     8221 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     8281 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     8341 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     8401 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     8461 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     8521 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     8581 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     8641 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     8701 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     8761 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     8821 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     8881 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     8941 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     9001 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     9061 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     9121 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     9181 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     9241 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     9301 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     9361 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9421 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     9481 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     9541 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     9601 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     9661 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     9721 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     9781 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     9841 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     9901 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     9961 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
    10021 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
    10081 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
    10141 ggattttgcc gatttcggcc tattggttaa a
//
