LOCUS       sequence-333022-        7785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmDCP2_209-791.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7785)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmDCP2_209-791_A
FEATURES             Location/Qualifiers
     source          1..7785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..2589
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2609..2611,2612..2614,2615..3325)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2609..2611,2612..2614,2615..3325)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2654..2675)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2915..2934)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3262..3283
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5115..5156
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5166..5183
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5206..5223)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5256..5390
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5280..5299)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5334..5353
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5991..6579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6071..6090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6750..7541,7542..7610))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7373..7392
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7611..7715)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(7783..7785,1..16)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aggcgtatca cgaggccctt tcgtcttcaa gaattaattc tatattctaa aaacacaaat
       61 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca
      121 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc
      181 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat
      241 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag
      301 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc
      361 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc
      421 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg
      481 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt
      541 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc
      601 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt
      661 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt
      721 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat
      781 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt
      841 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa
      901 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg
      961 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg
     1021 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc
     1081 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc
     1141 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt
     1201 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact
     1261 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc
     1321 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat
     1381 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg
     1441 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca
     1501 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt
     1561 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag
     1621 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct
     1681 ctctcttcgt ctctgttgcg caaacgcata gactgcactg aaaaaatcga ttacctattt
     1741 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca
     1801 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc
     1861 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct
     1921 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta
     1981 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa
     2041 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat
     2101 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac
     2161 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac
     2221 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga
     2281 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt
     2341 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg
     2401 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc
     2461 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc
     2521 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa
     2581 tccagagacc ccggatcggg gtaccatcat ggtgagcaag ggcgaggagc tgttcaccgg
     2641 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     2701 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     2761 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     2821 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     2881 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     2941 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3001 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3061 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     3121 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3181 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     3241 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3301 tctcggcatg gacgagctgt acaagccaag cttgattccg tccgatatac ttgatgatct
     3361 ggccagtcga tttataatca atgttccgga catggagcta aacaatctaa tacgtatgtg
     3421 tttccaaatc gaactggccc attggttcta tttggatttc ttttgtgccc ccgaatctgg
     3481 cgaggatggc gagaccccga agtgcgtgca acgaaagctg ccatcggtgg gcatcaagca
     3541 gtttgccatg cagttgttcc aacacattcc cttcctgaac aaacattttg gcaccgtgga
     3601 tcaaatcctg gacgagtgga agaactacaa gctatcggtg cccacttatg gcgctattct
     3661 ggtgtccgag gatcacaatc actgcttgct agtgcagtcc tacttcgcgc gcaactcctg
     3721 gggatttccc aagggcaaga tcaatgagaa cgaagaccca gcccattgtg caacgagaga
     3781 ggtttatgag gagaccgggt tcgatatcac ggacctaatc gatgccaacg actacatcga
     3841 ggcgttcatc aactaccagt acacgcggct gtacgtggtg cgaaacatac cgatggacac
     3901 gcagttcgcg ccccgcactc gcaacgagat caagtgctgc gactggttcc gtatcgatgc
     3961 gctgccggtg aacaagaacg atgccatatc aaaggccaag ttgggcaaga cctccaactc
     4021 cttcttcatg attatgccgt ttgtgaagcg cctgaagaag tgggtgaacg atcggaaggc
     4081 tggaatcgag ccgcgtcgcc gcaaattctc tggtcagcaa tcgccaaaag cggcctcgcc
     4141 caccaatatg aacggaaatt gcaagaagga cgtcacagga gtgcgaaatg aatcgcaacg
     4201 acagaggcac aagtccatgg gcgacttgga tggcgtcaaa ttgaataatc tcaatggcaa
     4261 ggggaccgtt ggaacgggag cgggatcggc agcttccgcc tccgccaccg ctgccatcaa
     4321 ctcgatgaac atatgcaata gcaatgggaa acgcaataag caaaatgcgg ctgcgggcag
     4381 caatcaggca acgccaacta caatagcaac gccaatgacg tcaaatggcg ccgtcgtagg
     4441 aggtactgta tcctcgaagc gtcaactgtt ccatagccaa agtcagaacg atgcgcagca
     4501 atcggcaagc aatgagaaga ttgtcagctc cttcgattta atacgcaagg agaagcagca
     4561 acaacttaag gcagctaagg cgcaaaagca gcagcaacaa cagcagcaac gatcgcgcac
     4621 caagtcgcag agtgataagc agtacaatcc acagcagcca gtcaaaatcc tgggacaaca
     4681 gcagcccaca acgggcatgg atttgatagc catgctatcg gcagcggcgg cagctcaaaa
     4741 gacaccaaac aacgagcagc aaacacaaga gaagcagcag cagcaacaac gaccacgacc
     4801 tgcttcagtg tcgcttaacc ttggagcagg cagtgccaca ggagcaacaa catcagctcc
     4861 ggatgcccag tacatgcaca ggctgcagcg catggcatcg ggcacaaatt tgaggattct
     4921 gaagcgggca cagtcgcagc agccgcagca gcaccatcag caacagttgc aactagaaca
     4981 gcaacaacag cagcagcagc agctgggaat cgactttacg ccaaatttga attcgtggac
     5041 gaatttctcc tttaccaaga acttcatagc aaatgtgttt tgctaactcg agtctagagg
     5101 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     5161 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     5221 ctaaggcctg agctcgctga tcagcctcga tcgaggatcc agacatgata agatacattg
     5281 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     5341 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     5401 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     5461 aaaacctcta caaatgtggt atggctgatt atgatcagtc gaccgatgcc cttgagagcc
     5521 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     5581 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     5641 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     5701 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     5761 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     5821 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     5881 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     5941 caaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6001 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6061 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6121 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6181 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6241 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6301 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6361 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6421 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6481 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6541 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6601 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6661 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6721 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6781 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6841 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6901 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6961 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7021 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     7081 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7141 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7201 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7261 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7321 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     7381 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7441 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7501 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7561 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7621 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7681 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7741 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaat
//
