LOCUS       sequence-333031-        7052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of CG7785-3UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7052)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1C-Fluc-CG7785_A
FEATURES             Location/Qualifiers
     source          1..7052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..2591
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2611..4266
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MAEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVA
                     PANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQ
                     SMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSH
                     ARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDY
                     KIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQG
                     YGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIM
                     SGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESI
                     LLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGV
                     VFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2680..2698)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     rep_origin      complement(5260..5848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5340..5359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6019..6810,6811..6879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6642..6661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6880..6984)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(7052,1..18)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ataggcgtat cacgaggccc tttcgtcttc aagaattaat tctatattct aaaaacacaa
       61 atgatacttc taaaaaaaaa tcatgaatgg catcaactct gaatcaaatc tttgcagatg
      121 cacctacttc tcatttccac tgtcacatca tttttccaga tctcgctgcc tgttatgtgg
      181 cccacaaacc aagacacgtt ttatggccat taaagctggc tgatcgtcgc caaacaccaa
      241 atacataatg aatatgtaca cattcgagaa agaagcgatc aaagaagcgt cttcgggcgg
      301 agtaggagaa tgcggaggag aaggagaacg agctgatcta gtatctctcc acaatccaat
      361 gccaactgac caactggcca tattcggagc aatttgaagc caatttccat cgcctggcga
      421 tcgctccatt cttggctata tgtttttcac cgttacccgg ggccattttc aaagactcgt
      481 cggcaagata agattgtgtc actcgctgtc tctcttcatt tgtcgaagaa tgctgaggaa
      541 tttcgcgatg acgtcggcga gtattttgaa gaatgagaat aatttgtatt tatacgaaaa
      601 tcagttagtg gaattttcta caaaaacatg ttatctatag ataattttgt tgcaaaatat
      661 gttgactatg acaaagattg tatgtatata cctttaatgt attctcattt tcttatgtat
      721 ttataatggc aatgatgata ctgatgatat tttaagatga tgccagacca aaaggcttga
      781 atttctgcgt cttttgccga acgcagtgca tgtgcaattg ttgttttttg gaatattcaa
      841 ttttcggact gtccgctttg atttcagttt cttggcttat tcaaaaagca aagtaaagcc
      901 aaaaaagcga gatggcaata ccaaatgcgg caaaacggta gtggaaggaa aggggtgcgg
      961 ggcagcggaa ggaagggtgg ggcggggcgt ggcggggtct gtggctgggc gcgacgtcac
     1021 cgacgttgga gccactcctt tgaccatgtg tgcgtgtgtg tattattcgt gtctcgccac
     1081 tcgccggttg tttttttctt tttatgctgc gctctctcta gcgccatctc gcttacgcat
     1141 gctcaacgca ccgcatgttg ccgtttcctt ttatgcgtca ttttggctcg aaataggcaa
     1201 ttatttaaac aaagattagt caacgaaaac gctaaaataa ataagtctac aatatggtta
     1261 cttattgcca tgtgtgtgca gccaacgata gcaacaaaag caacaacaca ggtggctttc
     1321 cctctttcac tttttgtttg caagccgcgt gcgagcaaga cggcacgacc ggcaaacgca
     1381 attacgctga caaagagcag acgaagtttt ggcgaaaaac atcaaggcgc ctgatacgaa
     1441 tgcatttgca ataacaattg cgatatttaa tattgtttat gaagctgttt gacttcaaaa
     1501 cacacaaaaa aaaaaataaa acaaattatt tgaaagagaa ttaggaatcg gacgcttatc
     1561 gttagggtaa caacaagaaa tgcttactga gtcacagcct ctggaaaact gccgcaagcc
     1621 agagagagag agaaaaagag ggagagcagc ttagaccgca tgtgcttgtg tgtgaggcgt
     1681 ctctctcttc gtctctgttg cgcaaacgca tagactgcac tgaaaaaatc gattacctat
     1741 tttttatgaa tgaatatttg cactattact attcaaaact attaagatag caatcacatt
     1801 caatagccaa atactatacc acctgagcga tgcaacgaaa tgatcaattt gagcaaaaat
     1861 gctgcatatt taggacggca tcattataga aatgcttctt gctgtgtact tttctctcgt
     1921 ctggcagctg tttcgccgtt attgttaaaa ccggcttaag ttaggtgtgt tttctacgac
     1981 tagtgaatgc cctactagaa gatgtgtgtt gcacaaaatg tccctggaat aaccaatttg
     2041 aagtgcagat agcagtaaac gtaagctaat atgaatatta tttaactgta atgttttaat
     2101 atcgctggac attactaata aacccactat aaacacatgt acatatgtat gttttggcat
     2161 acaatgagta gttggggaaa aaatgtgtaa aagcaccgtg accatcacag cataaagata
     2221 accagctgaa gtatcgaata tgagtaaccc ccaaattgaa tcacatgccg caactgatag
     2281 gacccatgga agtacactct tcatggcgat atacaagaca cacacaagca cgaacaccca
     2341 gttgcggagg aaattctccg taaatgaaaa cccaatcggc gaacaattca tacccatata
     2401 tggtaaaagt tttgaacgcg acttgagagc ggagagcatt gcggctgata aggttttagc
     2461 gctaagcggg ctttataaaa cgggctgcgg gaccagtttt catatcacta ccgtttgagt
     2521 tcttgtgctg tgtggatact cctcccgaca caaagccgct ccatcagcca gcagtcgtct
     2581 aatccagaga ccccggatcg gggtaccaac atggccgaag acgccaaaaa cataaagaaa
     2641 ggcccggcac cattctatcc actagaggat ggaaccgctg gagagcaact gcataaggct
     2701 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg
     2761 aacatcacgt acgcggaata cttcgaaatg tccgttcggt tggcagaagc tatgaaacga
     2821 tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt
     2881 atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat
     2941 aatgaacgtg aattgctcaa cagtatgaac atttcgcagc ctaccgtagt gtttgtttcc
     3001 aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaaat taccaataat ccagaaaatt
     3061 attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca
     3121 tctcatctac ctcccggttt taatgaatac gattttgtac cagagtcctt tgatcgtgac
     3181 aaaacaattg cactgataat gaactcctct ggatctactg ggttacctaa gggtgtggcc
     3241 cttccgcata gaactgcctg cgtcagattc tcgcatgcca gagatcctat ttttggcaat
     3301 caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg
     3361 tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa
     3421 gaagagctgt ttttacgatc ccttcaggat tacaaaattc aaagtgcgtt gctagtacca
     3481 accctatttt cattcttcgc caaaagcact ctgattgaca aatacgattt atctaattta
     3541 cacgaaattg cttctggggg cgcacctctt tcgaaagaag tcggggaagc ggttgcaaaa
     3601 cgcttccatc ttccagggat acgacaagga tatgggctca ctgagactac atcagctatt
     3661 ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt
     3721 gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca gagaggcgaa
     3781 ttatgtgtca gaggacctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac
     3841 gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac
     3901 gaacacttct tcatagttga ccgcttgaag tctttaatta aatacaaagg gtatcaggtg
     3961 gcccccgctg aattggaatc gatattgtta caacacccca acatcttcga cgcgggcgtg
     4021 gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac
     4081 ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg
     4141 aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa
     4201 ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagtccaaa
     4261 ttgtaagaat tctatctcac aaccaaaaca gctattaaat tatttcaatt tactttgtac
     4321 acatatctta atcgattttg ttgattttca ccacatattt acttatagtc gtaaaatgca
     4381 ctgttctgtt ctcatttaga catcaggtgt ataaactttg taaactctct agtccttgca
     4441 ctatccaaac cgaaagtatc tgtataattt tattaatgtt ttgtaaatta tttacgtagc
     4501 ttgacgtagc atttgtatta tacacagagg tacgcgtatc tgcatacaga tacagatgaa
     4561 tttttttgta ggactaggtg gccacaattc aattctggct tcatcgtaca cattccaatt
     4621 tacaacggca acgtactcga gcacggaatt acctaactaa atataagaat atatccgtat
     4681 atctaataaa ttgtatctac acgtatccaa cgcctcgaaa actgattaat ccgcgcgcgg
     4741 acaataaatc aatgttatgt acttaagtcg accgatgccc ttgagagcct tcaacccagt
     4801 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     4861 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
     4921 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
     4981 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
     5041 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
     5101 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
     5161 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggccagca
     5221 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5281 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5341 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5401 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5461 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5521 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5581 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5641 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5701 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5761 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5821 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5881 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5941 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6001 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6061 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6121 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6181 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6241 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6301 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     6361 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6421 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6481 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6541 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6601 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     6661 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6721 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6781 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6841 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6901 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6961 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     7021 aaccattatt atcatgacat taacctataa aa
//
