LOCUS       sequence-333035-        9206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express SARS-CoV-2 P.1 (Brazil / gamma) strain spike protein with an
            18aa deletion on the C-terminal tail. Used for SARS2 P.1 pseudotyped
            virus production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Yuan M, Huang D, Lee CD, Wu NC, Jackson AM, Zhu X, Liu H, Peng L, 
            van Gils MJ, Sanders RW, Burton DR, Reincke SM, Pruss H, Kreye J, 
            Nemazee D, Ward AB, Wilson IA
  TITLE     Structural and functional ramifications of antigenic drift in recent
            SARS-CoV-2 variants.
  JOURNAL   Science. 2021 Aug 13;373(6556):818-823. doi: 
            10.1126/science.abh1139. Epub 2021 May 20.
  PUBMED    34016740
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abh1139"; journalName: "Science"; date: 
            "2021-08-13- 13"; volume: "373"; issue: "6556"; pages: "818-823"
COMMENT     Sequence Label: pcDNA3.3_CoV2_P1
FEATURES             Location/Qualifiers
     source          1..9206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        80..459
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        460..663
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     614..634
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     660..684
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             complement(2102..2119)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     primer_bind     complement(4622..4641)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4666..4714
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4916..5344
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5003..5022)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5213..5234
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5353..5373)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5358..5687
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5538..5673
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5600..5619
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5754..6548
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5808..5827)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6418..6437
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6724..6845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6761..6780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6815..6834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6894..6910)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6894..6910)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6907..6929)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6918..6934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6942..6948,6949..6966,6967..6972))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6987..7008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7125..7142)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7296..7884)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7376..7395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8055..8846,8847..8915))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8678..8697
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8916..9020)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9095..9114)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
       61 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      121 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      181 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      241 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      301 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      361 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      421 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      481 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      541 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      601 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      661 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      721 agaagacacc gggaccgatc cagcctccgg actgagctcc ttaaggttaa ctctagagaa
      781 ttcggatcca tgtttgtgtt cctggtgctg ctgccactgg tgtccagcca gtgtgtgaac
      841 ttcaccaaca ggacccaact tccttctgcc tacaccaact ccttcaccag gggagtctac
      901 taccctgaca aggtgttcag gtcctctgtg ctgcacagca cccaggacct gttcctgcca
      961 ttcttcagca atgtgacctg gttccatgcc atccatgtgt ctggcaccaa tggcaccaag
     1021 aggtttgaca accctgtgct gccattcaat gatggagtct actttgccag cacagagaag
     1081 agcaacatca tcaggggctg gatttttggc accaccctgg acagcaagac ccagtccctg
     1141 ctgattgtga acaatgccac caatgtggtg attaaggtgt gtgagttcca gttctgtaat
     1201 tacccattcc tgggagtcta ctaccacaag aacaacaagt cctggatgga gtctgagttc
     1261 agggtctact cctctgccaa caactgtacc tttgaatatg tgagccaacc attcctgatg
     1321 gacttggagg gcaagcaggg caacttcaag aacctgtcgg agtttgtgtt caagaacatt
     1381 gatggctact tcaagattta cagcaaacac acaccaatca acctggtgag ggacctgcca
     1441 cagggcttct ctgccttgga accactggtg gacctgccaa ttggcatcaa catcaccagg
     1501 ttccagaccc tgctggctct gcacaggtcc tacctgacac ctggagactc ctcctctggc
     1561 tggacagcag gagcagcagc ctactatgtg ggctacctcc aaccaaggac cttcctgctg
     1621 aaatacaatg agaatggcac catcacagat gctgtggact gtgccctgga cccactgtct
     1681 gagaccaagt gtaccctgaa atccttcaca gtggagaagg gcatctacca gaccagcaac
     1741 ttcagggtcc aaccaacaga gagcattgtg aggtttccaa acatcaccaa cctgtgtcca
     1801 tttggagagg tgttcaatgc caccaggttt gcctctgtct atgcctggaa caggaagagg
     1861 attagcaact gtgtggctga ctactctgtg ctctacaact ctgcctcctt cagcaccttc
     1921 aagtgttatg gagtgagccc aaccaaactg aatgacctgt gtttcaccaa tgtctatgct
     1981 gactcctttg tgattagggg agatgaggtg agacagattg cccctggaca aacaggcacg
     2041 attgctgact acaactacaa actgcctgat gacttcacag gctgtgtgat tgcctggaac
     2101 agcaacaacc tggacagcaa ggtgggaggc aactacaact acctctacag actgttcagg
     2161 aagagcaacc tgaaaccatt tgagagggac atcagcacag agatttacca ggctggcagc
     2221 acaccatgta atggagtgaa gggcttcaac tgttactttc cactccaatc ctatggcttc
     2281 caaccaacct atggagtggg ctaccaacca tacagggtgg tggtgctgtc ctttgaactg
     2341 ctccatgccc ctgccacagt gtgtggacca aagaagagca ccaacctggt gaagaacaag
     2401 tgtgtgaact tcaacttcaa tggactgaca ggcacaggag tgctgacaga gagcaacaag
     2461 aagttcctgc cattccaaca gtttggcagg gacattgctg acaccacaga tgctgtgagg
     2521 gacccacaga ccttggagat tctggacatc acaccatgtt cctttggagg agtgtctgtg
     2581 attacacctg gcaccaacac cagcaaccag gtggctgtgc tctaccaggg tgtgaactgt
     2641 actgaggtgc ctgtggctat ccatgctgac caacttacac caacctggag ggtctacagc
     2701 acaggcagca atgtgttcca gaccagggct ggctgtctga ttggagcaga gtatgtgaac
     2761 aactcctatg agtgtgacat cccaattgga gcaggcatct gtgcctccta ccagacccag
     2821 accaacagcc caaggagggc aaggtctgtg gcaagccaga gcatcattgc ctacacaatg
     2881 agtctgggag cagagaactc tgtggcttac agcaacaaca gcattgccat cccaaccaac
     2941 ttcaccatct ctgtgaccac agagattctg cctgtgagta tgaccaagac ctctgtggac
     3001 tgtacaatgt atatctgtgg agacagcaca gagtgtagca acctgctgct ccaatatggc
     3061 tccttctgta cccaacttaa cagggctctg acaggcattg ctgtggaaca ggacaagaac
     3121 acccaggagg tgtttgccca ggtgaagcag atttacaaga cacctccaat caaggacttt
     3181 ggaggcttca acttcagcca gattctgcct gacccaagca agccaagcaa gaggtccttc
     3241 attgaggacc tgctgttcaa caaggtgacc ctggctgatg ctggcttcat caagcaatat
     3301 ggagactgtc tgggagacat tgctgccagg gacctgattt gtgcccagaa gttcaatgga
     3361 ctgacagtgc tgcctccact gctgacagat gagatgattg cccaatacac ctctgccctg
     3421 ctggctggca ccatcacctc tggctggacc tttggagcag gagcagccct ccaaatccca
     3481 tttgctatgc agatggctta caggttcaat ggcattggag tgacccagaa tgtgctctat
     3541 gagaaccaga aactgattgc caaccagttc aactctgcca ttggcaagat tcaggactcc
     3601 ctgtccagca cagcctctgc cctgggcaaa ctccaagatg tggtgaacca gaatgcccag
     3661 gctctgaaca ccctggtgaa gcaactttcc agcaactttg gagccatctc ctctgtgctg
     3721 aatgacatcc tgagcagact ggacaaggtg gaggctgagg tccagattga cagactgatt
     3781 acaggcagac tccaatccct ccaaacctat gtgacccaac aacttatcag ggctgctgag
     3841 attagggcat ctgccaacct ggctgccatc aagatgagtg agtgtgtgct gggacaaagc
     3901 aagagggtgg acttctgtgg caagggctac cacctgatga gttttccaca gtctgcccct
     3961 catggagtgg tgttcctgca tgtgacctat gtgcctgccc aggagaagaa cttcaccaca
     4021 gcccctgcca tctgccatga tggcaaggct cactttccaa gggagggagt gtttgtgagc
     4081 aatggcaccc actggtttgt gacccagagg aacttctatg aaccacagat tatcaccaca
     4141 gacaacacct ttgtgtctgg caactgtgat gtggtgattg gcattgtgaa caacacagtc
     4201 tatgacccac tccaacctga actggactcc ttcaaggagg aactggacaa atacttcaag
     4261 aaccacacca gccctgatgt ggacctggga gacatctctg gcatcaatgc ctctgtggtg
     4321 aacatccaga aggagattga cagactgaat gaggtggcta agaacctgaa tgagtccctg
     4381 attgacctcc aagaactggg caaatatgaa caatacatca agtggccatg gtacatctgg
     4441 ctgggcttca ttgctggact gattgccatt gtgatggtga ccataatgct gtgttgtatg
     4501 acctcctgtt gttcctgtct gaaaggctgt tgttcctgtg gctcctgttg taagtgactc
     4561 gagccatgga agcttgatat ctaactgact gaaccggtgg taccaaacgg gggaggctaa
     4621 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     4681 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     4741 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     4801 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     4861 gcggcaggcc ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg
     4921 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4981 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5041 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5101 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5161 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5221 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5281 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5341 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5401 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5461 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5521 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5581 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     5641 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     5701 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     5761 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5821 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5881 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5941 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     6001 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     6061 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     6121 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     6181 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     6241 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     6301 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     6361 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     6421 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     6481 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     6541 tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca
     6601 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6661 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6721 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6781 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6841 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6901 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6961 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7021 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7081 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7141 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7201 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7261 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7321 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7381 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7441 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7501 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7561 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7621 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7681 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7741 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7801 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7861 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7921 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7981 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8041 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8101 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8161 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8221 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8281 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8341 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8401 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8461 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8521 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8581 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8641 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8701 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8761 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8821 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8881 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8941 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9001 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     9061 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9121 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     9181 tttaagctac aacaaggcaa ggcttg
//
