LOCUS       sequence-333037-        5762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmLSm3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Tritschler F, Eulalio A, Truffault V, Hartmann MD, Helms S, Schmidt 
            S, Coles M, Izaurralde E, Weichenrieder O
  TITLE     A divergent Sm fold in EDC3 proteins mediates DCP1 binding and 
            P-body targeting.
  JOURNAL   Mol Cell Biol. 2007 Dec . 27(24):8600-11.
  PUBMED    17923697
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Dec . 27(24):8600-11."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmLSm3_A
FEATURES             Location/Qualifiers
     source          1..5762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        44..2553
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2573..2638
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2663..2689
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3056..3097
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3107..3124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3147..3164)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3197..3331
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3221..3240)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3275..3294
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3932..4520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4012..4031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4691..5482,5483..5551))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5314..5333
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5552..5656)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5724..5742
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 attctatatt ctaaaaacac aaatgatact tctaaaaaaa aatcatgaat ggcatcaact
       61 ctgaatcaaa tctttgcaga tgcacctact tctcatttcc actgtcacat catttttcca
      121 gatctcgctg cctgttatgt ggcccacaaa ccaagacacg ttttatggcc attaaagctg
      181 gctgatcgtc gccaaacacc aaatacataa tgaatatgta cacattcgag aaagaagcga
      241 tcaaagaagc gtcttcgggc ggagtaggag aatgcggagg agaaggagaa cgagctgatc
      301 tagtatctct ccacaatcca atgccaactg accaactggc catattcgga gcaatttgaa
      361 gccaatttcc atcgcctggc gatcgctcca ttcttggcta tatgtttttc accgttaccc
      421 ggggccattt tcaaagactc gtcggcaaga taagattgtg tcactcgctg tctctcttca
      481 tttgtcgaag aatgctgagg aatttcgcga tgacgtcggc gagtattttg aagaatgaga
      541 ataatttgta tttatacgaa aatcagttag tggaattttc tacaaaaaca tgttatctat
      601 agataatttt gttgcaaaat atgttgacta tgacaaagat tgtatgtata tacctttaat
      661 gtattctcat tttcttatgt atttataatg gcaatgatga tactgatgat attttaagat
      721 gatgccagac caaaaggctt gaatttctgc gtcttttgcc gaacgcagtg catgtgcaat
      781 tgttgttttt tggaatattc aattttcgga ctgtccgctt tgatttcagt ttcttggctt
      841 attcaaaaag caaagtaaag ccaaaaaagc gagatggcaa taccaaatgc ggcaaaacgg
      901 tagtggaagg aaaggggtgc ggggcagcgg aaggaagggt ggggcggggc gtggcggggt
      961 ctgtggctgg gcgcgacgtc accgacgttg gagccactcc tttgaccatg tgtgcgtgtg
     1021 tgtattattc gtgtctcgcc actcgccggt tgtttttttc tttttatgct gcgctctctc
     1081 tagcgccatc tcgcttacgc atgctcaacg caccgcatgt tgccgtttcc ttttatgcgt
     1141 cattttggct cgaaataggc aattatttaa acaaagatta gtcaacgaaa acgctaaaat
     1201 aaataagtct acaatatggt tacttattgc catgtgtgtg cagccaacga tagcaacaaa
     1261 agcaacaaca caggtggctt tccctctttc actttttgtt tgcaagccgc gtgcgagcaa
     1321 gacggcacga ccggcaaacg caattacgct gacaaagagc agacgaagtt ttggcgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacgctta tcgttagggt aacaacaaga aatgcttact gagtcacagc
     1561 ctctggaaaa ctgccgcaag ccagagagag agagaaaaag agggagagca gcttagaccg
     1621 catgtgcttg tgtgtgaggc gtctctctct tcgtctctgt tgcgcaaacg catagactgc
     1681 actgaaaaaa tcgattacct attttttatg aatgaatatt tgcactatta ctattcaaaa
     1741 ctattaagat agcaatcaca ttcaatagcc aaatactata ccacctgagc gatgcaacga
     1801 aatgatcaat ttgagcaaaa atgctgcata tttaggacgg catcattata gaaatgcttc
     1861 ttgctgtgta cttttctctc gtctggcagc tgtttcgccg ttattgttaa aaccggctta
     1921 agttaggtgt gttttctacg actagtgaat gccctactag aagatgtgtg ttgcacaaaa
     1981 tgtccctgga ataaccaatt tgaagtgcag atagcagtaa acgtaagcta atatgaatat
     2041 tatttaactg taatgtttta atatcgctgg acattactaa taaacccact ataaacacat
     2101 gtacatatgt atgttttggc atacaatgag tagttgggga aaaaatgtgt aaaagcaccg
     2161 tgaccatcac agcataaaga taaccagctg aagtatcgaa tatgagtaac ccccaaattg
     2221 aatcacatgc cgcaactgat aggacccatg gaagtacact cttcatggcg atatacaaga
     2281 cacacacaag cacgaacacc cagttgcgga ggaaattctc cgtaaatgaa aacccaatcg
     2341 gcgaacaatt catacccata tatggtaaaa gttttgaacg cgacttgaga gcggagagca
     2401 ttgcggctga taaggtttta gcgctaagcg ggctttataa aacgggctgc gggaccagtt
     2461 ttcatatcac taccgtttga gttcttgtgc tgtgtggata ctcctcccga cacaaagccg
     2521 ctccatcagc cagcagtcgt ctaatccaga gaccccggat cggggtacca acatggacgc
     2581 acaaacacga cgacgtgagc gtcgcgctga gaaacaagct caatggaaag ctgcaaacgg
     2641 tggatctcct ccacatatgg cttacccata cgatgttcca gattacgctc ctccaagctt
     2701 gaattctatg gccgacgaaa cagagcagct cagtcaggtg attttgccgg taaaggagcc
     2761 tttggatctc atccgattga gtttagatga gaaggtgtac gtaaagatgc gcaacgagcg
     2821 ggaactgcga ggacgtcttc acgcctttga tcagcatttg aacatggtgc tcggcgatgc
     2881 ggaggagacg gtgaccactg tggagatcga cgaggagacc tatgaggagg tgtacaagac
     2941 cgccaagcgc actattccca tgctattcgt tagaggcgat ggagtcatcc tggtttcgcc
     3001 acccatgcgg gtgggctagg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa
     3061 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca
     3121 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctaaggcct gagctcgctg
     3181 atcagcctcg atcgaggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     3241 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     3301 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     3361 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     3421 tatggctgat tatgatcagt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     3481 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     3541 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
     3601 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
     3661 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
     3721 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
     3781 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
     3841 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggccag caaaaggcca
     3901 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3961 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4021 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4081 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4141 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4201 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4261 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4321 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     4381 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4441 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4501 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4561 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4621 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4681 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4741 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4801 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4861 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4921 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4981 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     5041 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5101 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5161 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5221 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5281 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     5341 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5401 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5461 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5521 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5581 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5641 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     5701 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat
     5761 ta
//
