LOCUS       sequence-333038-        9197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein 
            with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK 
            strain) pseudotyped virus production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Yuan M, Huang D, Lee CD, Wu NC, Jackson AM, Zhu X, Liu H, Peng L, 
            van Gils MJ, Sanders RW, Burton DR, Reincke SM, Pruss H, Kreye J, 
            Nemazee D, Ward AB, Wilson IA
  TITLE     Structural and functional ramifications of antigenic drift in recent
            SARS-CoV-2 variants.
  JOURNAL   Science. 2021 Aug 13;373(6556):818-823. doi: 
            10.1126/science.abh1139. Epub 2021 May 20.
  PUBMED    34016740
REFERENCE   2  (bases 1 to 9197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abh1139"; journalName: "Science"; date: 
            "2021-08-13- 13"; volume: "373"; issue: "6556"; pages: "818-823"
COMMENT     Sequence Label: pCDNA3.3_CoV2_B.1.1.7
FEATURES             Location/Qualifiers
     source          1..9197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..40)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(194..782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(274..293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(953..1744,1745..1813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1576..1595
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1814..1918)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1993..2012)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2184..2563
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2564..2767
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2718..2738
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2764..2788
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             complement(4197..4214)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     primer_bind     complement(6717..6736)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6761..6809
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7011..7439
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7098..7117)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7308..7329
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7448..7468)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7453..7782
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7633..7768
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7695..7714
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7849..8643
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7903..7922)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8513..8532
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8819..8940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8856..8875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8910..8929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8989..9005)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8989..9005)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9002..9024)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9013..9029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9037..9043,9044..9061,9062..9067))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9082..9103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
       61 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      121 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      181 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      241 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      301 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      361 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      421 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      481 ccttatccgg taactatcgt cttgagtcca acccggtaag acacggctta tcgccactgg
      541 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      601 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      661 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      721 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      781 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      841 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      901 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      961 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1021 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1081 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1141 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1201 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1261 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1321 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1381 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1441 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1501 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1561 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1621 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1681 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1741 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1801 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1861 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1921 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     1981 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     2041 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     2101 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     2161 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     2221 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     2281 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2341 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2401 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2461 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2521 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2581 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2641 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2701 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2761 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2821 ccatagaaga caccgggacc gatccagcct ccggactgag ctccttaagg ttaactctag
     2881 agaattcgga tccatgtttg tgttcctggt gctgctgcca ctggtgtcca gccagtgtgt
     2941 gaacctgacc accaggaccc aacttcctcc tgcctacacc aactccttca ccaggggagt
     3001 ctactaccct gacaaggtgt tcaggtcctc tgtgctgcac agcacccagg acctgttcct
     3061 gccattcttc agcaatgtga cctggttcca tgccatctct ggcaccaatg gcaccaagag
     3121 gtttgacaac cctgtgctgc cattcaatga tggagtctac tttgccagca cagagaagag
     3181 caacatcatc aggggctgga tttttggcac caccctggac agcaagaccc agtccctgct
     3241 gattgtgaac aatgccacca atgtggtgat taaggtgtgt gagttccagt tctgtaatga
     3301 cccattcctg ggagtctacc acaagaacaa caagtcctgg atggagtctg agttcagggt
     3361 ctactcctct gccaacaact gtacctttga atatgtgagc caaccattcc tgatggactt
     3421 ggagggcaag cagggcaact tcaagaacct gagggagttt gtgttcaaga acattgatgg
     3481 ctacttcaag atttacagca aacacacacc aatcaacctg gtgagggacc tgccacaggg
     3541 cttctctgcc ttggaaccac tggtggacct gccaattggc atcaacatca ccaggttcca
     3601 gaccctgctg gctctgcaca ggtcctacct gacacctgga gactcctcct ctggctggac
     3661 agcaggagca gcagcctact atgtgggcta cctccaacca aggaccttcc tgctgaaata
     3721 caatgagaat ggcaccatca cagatgctgt ggactgtgcc ctggacccac tgtctgagac
     3781 caagtgtacc ctgaaatcct tcacagtgga gaagggcatc taccagacca gcaacttcag
     3841 ggtccaacca acagagagca ttgtgaggtt tccaaacatc accaacctgt gtccatttgg
     3901 agaggtgttc aatgccacca ggtttgcctc tgtctatgcc tggaacagga agaggattag
     3961 caactgtgtg gctgactact ctgtgctcta caactctgcc tccttcagca ccttcaagtg
     4021 ttatggagtg agcccaacca aactgaatga cctgtgtttc accaatgtct atgctgactc
     4081 ctttgtgatt aggggagatg aggtgagaca gattgcccct ggacaaacag gcaagattgc
     4141 tgactacaac tacaaactgc ctgatgactt cacaggctgt gtgattgcct ggaacagcaa
     4201 caacctggac agcaaggtgg gaggcaacta caactacctc tacagactgt tcaggaagag
     4261 caacctgaaa ccatttgaga gggacatcag cacagagatt taccaggctg gcagcacacc
     4321 atgtaatgga gtggagggct tcaactgtta ctttccactc caatcctatg gcttccaacc
     4381 aacctatgga gtgggctacc aaccatacag ggtggtggtg ctgtcctttg aactgctcca
     4441 tgcccctgcc acagtgtgtg gaccaaagaa gagcaccaac ctggtgaaga acaagtgtgt
     4501 gaacttcaac ttcaatggac tgacaggcac aggagtgctg acagagagca acaagaagtt
     4561 cctgccattc caacagtttg gcagggacat tgatgacacc acagatgctg tgagggaccc
     4621 acagaccttg gagattctgg acatcacacc atgttccttt ggaggagtgt ctgtgattac
     4681 acctggcacc aacaccagca accaggtggc tgtgctctac cagggtgtga actgtactga
     4741 ggtgcctgtg gctatccatg ctgaccaact tacaccaacc tggagggtct acagcacagg
     4801 cagcaatgtg ttccagacca gggctggctg tctgattgga gcagagcatg tgaacaactc
     4861 ctatgagtgt gacatcccaa ttggagcagg catctgtgcc tcctaccaga cccagaccaa
     4921 cagccatagg agggcaaggt ctgtggcaag ccagagcatc attgcctaca caatgagtct
     4981 gggagcagag aactctgtgg cttacagcaa caacagcatt gccatcccaa tcaacttcac
     5041 catctctgtg accacagaga ttctgcctgt gagtatgacc aagacctctg tggactgtac
     5101 aatgtatatc tgtggagaca gcacagagtg tagcaacctg ctgctccaat atggctcctt
     5161 ctgtacccaa cttaacaggg ctctgacagg cattgctgtg gaacaggaca agaacaccca
     5221 ggaggtgttt gcccaggtga agcagattta caagacacct ccaatcaagg actttggagg
     5281 cttcaacttc agccagattc tgcctgaccc aagcaagcca agcaagaggt ccttcattga
     5341 ggacctgctg ttcaacaagg tgaccctggc tgatgctggc ttcatcaagc aatatggaga
     5401 ctgtctggga gacattgctg ccagggacct gatttgtgcc cagaagttca atggactgac
     5461 agtgctgcct ccactgctga cagatgagat gattgcccaa tacacctctg ccctgctggc
     5521 tggcaccatc acctctggct ggacctttgg agcaggagca gccctccaaa tcccatttgc
     5581 tatgcagatg gcttacaggt tcaatggcat tggagtgacc cagaatgtgc tctatgagaa
     5641 ccagaaactg attgccaacc agttcaactc tgccattggc aagattcagg actccctgtc
     5701 cagcacagcc tctgccctgg gcaaactcca agatgtggtg aaccagaatg cccaggctct
     5761 gaacaccctg gtgaagcaac tttccagcaa ctttggagcc atctcctctg tgctgaatga
     5821 catcctggcc agactggaca aggtggaggc tgaggtccag attgacagac tgattacagg
     5881 cagactccaa tccctccaaa cctatgtgac ccaacaactt atcagggctg ctgagattag
     5941 ggcatctgcc aacctggctg ccaccaagat gagtgagtgt gtgctgggac aaagcaagag
     6001 ggtggacttc tgtggcaagg gctaccacct gatgagtttt ccacagtctg cccctcatgg
     6061 agtggtgttc ctgcatgtga cctatgtgcc tgcccaggag aagaacttca ccacagcccc
     6121 tgccatctgc catgatggca aggctcactt tccaagggag ggagtgtttg tgagcaatgg
     6181 cacccactgg tttgtgaccc agaggaactt ctatgaacca cagattatca ccacacacaa
     6241 cacctttgtg tctggcaact gtgatgtggt gattggcatt gtgaacaaca cagtctatga
     6301 cccactccaa cctgaactgg actccttcaa ggaggaactg gacaaatact tcaagaacca
     6361 caccagccct gatgtggacc tgggagacat ctctggcatc aatgcctctg tggtgaacat
     6421 ccagaaggag attgacagac tgaatgaggt ggctaagaac ctgaatgagt ccctgattga
     6481 cctccaagaa ctgggcaaat atgaacaata catcaagtgg ccatggtaca tctggctggg
     6541 cttcattgct ggactgattg ccattgtgat ggtgaccata atgctgtgtt gtatgacctc
     6601 ctgttgttcc tgtctgaaag gctgttgttc ctgtggctcc tgttgtaagt gactcgagcc
     6661 atggaagctt gatatctaac tgactgaacc ggtggtacca aacgggggag gctaactgaa
     6721 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     6781 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     6841 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     6901 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     6961 aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc acgcgccctg
     7021 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7081 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7141 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7201 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7261 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7321 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7381 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     7441 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7501 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7561 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7621 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7681 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     7741 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     7801 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     7861 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     7921 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     7981 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     8041 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     8101 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     8161 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     8221 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     8281 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     8341 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     8401 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     8461 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     8521 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     8581 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     8641 tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     8701 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     8761 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     8821 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8881 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8941 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     9001 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9061 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9121 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9181 ggggagaggc ggtttgc
//
