LOCUS       sequence-333042-        9206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express SARS-CoV-2 501V2 (South Africa strain / beta strain) spike 
            protein with an 18aa deletion on the C-terminal tail. Used for SARS2
            501V2 pseudotyped virus production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Yuan M, Huang D, Lee CD, Wu NC, Jackson AM, Zhu X, Liu H, Peng L, 
            van Gils MJ, Sanders RW, Burton DR, Reincke SM, Pruss H, Kreye J, 
            Nemazee D, Ward AB, Wilson IA
  TITLE     Structural and functional ramifications of antigenic drift in recent
            SARS-CoV-2 variants.
  JOURNAL   Science. 2021 Aug 13;373(6556):818-823. doi: 
            10.1126/science.abh1139. Epub 2021 May 20.
  PUBMED    34016740
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abh1139"; journalName: "Science"; date: 
            "2021-08-13- 13"; volume: "373"; issue: "6556"; pages: "818-823"
COMMENT     Sequence Label: pcDNA3.3_CoV2_501V2
FEATURES             Location/Qualifiers
     source          1..9206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        165..544
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        545..748
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     699..719
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     745..769
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             complement(2187..2204)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     primer_bind     complement(4707..4726)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4751..4799
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5001..5429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5088..5107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5298..5319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5438..5458)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5443..5772
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5623..5758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5685..5704
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5839..6633
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5893..5912)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6503..6522
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6809..6930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6846..6865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6900..6919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6979..6995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6979..6995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6992..7014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7003..7019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7027..7033,7034..7051,7052..7057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7072..7093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7210..7227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7381..7969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7461..7480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8140..8931,8932..9000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8763..8782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9001..9105)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9180..9199)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
       61 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
      121 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      181 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      241 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      301 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      361 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      421 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      481 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      541 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      601 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      661 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      721 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      781 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactga
      841 gctccttaag gttaactcta gagaattcgg atccatgttt gtgttcctgg tgctgctgcc
      901 actggtgtcc agccagtgtg tgaacttcac caccaggacc caacttcctc ctgcctacac
      961 caactccttc accaggggag tctactaccc tgacaaggtg ttcaggtcct ctgtgctgca
     1021 cagcacccag gacctgttcc tgccattctt cagcaatgtg acctggttcc atgccatcca
     1081 tgtgtctggc accaatggca ccaagaggtt tgccaaccct gtgctgccat tcaatgatgg
     1141 agtctacttt gccagcacag agaagagcaa catcatcagg ggctggattt ttggcaccac
     1201 cctggacagc aagacccagt ccctgctgat tgtgaacaat gccaccaatg tggtgattaa
     1261 ggtgtgtgag ttccagttct gtaatgaccc attcctggga gtctactacc acaagaacaa
     1321 caagtcctgg atggagtctg agttcagggt ctactcctct gccaacaact gtacctttga
     1381 atatgtgagc caaccattcc tgatggactt ggagggcaag cagggcaact tcaagaacct
     1441 gagggagttt gtgttcaaga acattgatgg ctacttcaag atttacagca aacacacacc
     1501 aatcaacctg gtgaggggcc tgccacaggg cttctctgcc ttggaaccac tggtggacct
     1561 gccaattggc atcaacatca ccaggttcca gaccctgctg gctctgcaca tctcctacct
     1621 gacacctgga gactcctcct ctggctggac agcaggagca gcagcctact atgtgggcta
     1681 cctccaacca aggaccttcc tgctgaaata caatgagaat ggcaccatca cagatgctgt
     1741 ggactgtgcc ctggacccac tgtctgagac caagtgtacc ctgaaatcct tcacagtgga
     1801 gaagggcatc taccagacca gcaacttcag ggtccaacca acagagagca ttgtgaggtt
     1861 tccaaacatc accaacctgt gtccatttgg agaggtgttc aatgccacca ggtttgcctc
     1921 tgtctatgcc tggaacagga agaggattag caactgtgtg gctgactact ctgtgctcta
     1981 caactctgcc tccttcagca ccttcaagtg ttatggagtg agcccaacca aactgaatga
     2041 cctgtgtttc accaatgtct atgctgactc ctttgtgatt aggggagatg aggtgagaca
     2101 gattgcccct ggacaaacag gcaacattgc tgactacaac tacaaactgc ctgatgactt
     2161 cacaggctgt gtgattgcct ggaacagcaa caacctggac agcaaggtgg gaggcaacta
     2221 caactacctc tacagactgt tcaggaagag caacctgaaa ccatttgaga gggacatcag
     2281 cacagagatt taccaggctg gcagcacacc atgtaatgga gtgaagggct tcaactgtta
     2341 ctttccactc caatcctatg gcttccaacc aacctatgga gtgggctacc aaccatacag
     2401 ggtggtggtg ctgtcctttg aactgctcca tgcccctgcc acagtgtgtg gaccaaagaa
     2461 gagcaccaac ctggtgaaga acaagtgtgt gaacttcaac ttcaatggac tgacaggcac
     2521 aggagtgctg acagagagca acaagaagtt cctgccattc caacagtttg gcagggacat
     2581 tgctgacacc acagatgctg tgagggaccc acagaccttg gagattctgg acatcacacc
     2641 atgttccttt ggaggagtgt ctgtgattac acctggcacc aacaccagca accaggtggc
     2701 tgtgctctac cagggtgtga actgtactga ggtgcctgtg gctatccatg ctgaccaact
     2761 tacaccaacc tggagggtct acagcacagg cagcaatgtg ttccagacca gggctggctg
     2821 tctgattgga gcagagcatg tgaacaactc ctatgagtgt gacatcccaa ttggagcagg
     2881 catctgtgcc tcctaccaga cccagaccaa cagcccaagg agggcaaggt ctgtggcaag
     2941 ccagagcatc attgcctaca caatgagtct gggagtagag aactctgtgg cttacagcaa
     3001 caacagcatt gccatcccaa ccaacttcac catctctgtg accacagaga ttctgcctgt
     3061 gagtatgacc aagacctctg tggactgtac aatgtatatc tgtggagaca gcacagagtg
     3121 tagcaacctg ctgctccaat atggctcctt ctgtacccaa cttaacaggg ctctgacagg
     3181 cattgctgtg gaacaggaca agaacaccca ggaggtgttt gcccaggtga agcagattta
     3241 caagacacct ccaatcaagg actttggagg cttcaacttc agccagattc tgcctgaccc
     3301 aagcaagcca agcaagaggt ccttcattga ggacctgctg ttcaacaagg tgaccctggc
     3361 tgatgctggc ttcatcaagc aatatggaga ctgtctggga gacattgctg ccagggacct
     3421 gatttgtgcc cagaagttca atggactgac agtgctgcct ccactgctga cagatgagat
     3481 gattgcccaa tacacctctg ccctgctggc tggcaccatc acctctggct ggacctttgg
     3541 agcaggagca gccctccaaa tcccatttgc tatgcagatg gcttacaggt tcaatggcat
     3601 tggagtgacc cagaatgtgc tctatgagaa ccagaaactg attgccaacc agttcaactc
     3661 tgccattggc aagattcagg actccctgtc cagcacagcc tctgccctgg gcaaactcca
     3721 agatgtggtg aaccagaatg cccaggctct gaacaccctg gtgaagcaac tttccagcaa
     3781 ctttggagcc atctcctctg tgctgaatga catcctgagc agactggaca aggtggaggc
     3841 tgaggtccag attgacagac tgattacagg cagactccaa tccctccaaa cctatgtgac
     3901 ccaacaactt atcagggctg ctgagattag ggcatctgcc aacctggctg ccaccaagat
     3961 gagtgagtgt gtgctgggac aaagcaagag ggtggacttc tgtggcaagg gctaccacct
     4021 gatgagtttt ccacagtctg cccctcatgg agtggtgttc ctgcatgtga cctatgtgcc
     4081 tgcccaggag aagaacttca ccacagcccc tgccatctgc catgatggca aggctcactt
     4141 tccaagggag ggagtgtttg tgagcaatgg cacccactgg tttgtgaccc agaggaactt
     4201 ctatgaacca cagattatca ccacagacaa cacctttgtg tctggcaact gtgatgtggt
     4261 gattggcatt gtgaacaaca cagtctatga cccactccaa cctgaactgg actccttcaa
     4321 ggaggaactg gacaaatact tcaagaacca caccagccct gatgtggacc tgggagacat
     4381 ctctggcatc aatgcctctg tggtgaacat ccagaaggag attgacagac tgaatgaggt
     4441 ggctaagaac ctgaatgagt ccctgattga cctccaagaa ctgggcaaat atgaacaata
     4501 catcaagtgg ccatggtaca tctggctggg cttcattgct ggactgattg ccattgtgat
     4561 ggtgaccata atgctgtgtt gtatgacctc ctgttgttcc tgtctgaaag gctgttgttc
     4621 ctgtggctcc tgttgtaagt gactcgagcc atggaagctt gatatctaac tgactgaacc
     4681 ggtggtacca aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     4741 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
     4801 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     4861 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     4921 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg
     4981 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5041 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5101 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5161 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5221 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5281 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5341 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5401 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     5461 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5521 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5581 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5641 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5701 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5761 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     5821 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     5881 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     5941 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     6001 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     6061 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     6121 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     6181 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     6241 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     6301 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     6361 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     6421 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     6481 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     6541 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     6601 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gcgaaatgac
     6661 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     6721 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     6781 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     6841 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6901 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     6961 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7021 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7081 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7141 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7201 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7261 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7321 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7381 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7501 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7561 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7621 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7681 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7741 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7801 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7861 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7981 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8041 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8101 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8161 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8221 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8281 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8341 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8401 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8461 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8521 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8581 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8641 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8701 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8761 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8821 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8881 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8941 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9001 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9061 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9121 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     9181 acaatctgct ctgatgccgc atagtt
//
