LOCUS       sequence-333044-        7035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmExu.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7035)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmExu_B
FEATURES             Location/Qualifiers
     source          1..7035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        75..2584
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2604..2669
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2694..2720
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4360..4401
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4411..4428
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4451..4468)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4501..4635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4525..4544)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4579..4598
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5236..5824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5316..5335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5995..6786,6787..6855))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6618..6637
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6856..6960)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(7028..7035,1..11)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tatcacgagg ccctttcgtc ttcaagaatt aattctatat tctaaaaaca caaatgatac
       61 ttctaaaaaa aaatcatgaa tggcatcaac tctgaatcaa atctttgcag atgcacctac
      121 ttctcatttc cactgtcaca tcatttttcc agatctcgct gcctgttatg tggcccacaa
      181 accaagacac gttttatggc cattaaagct ggctgatcgt cgccaaacac caaatacata
      241 atgaatatgt acacattcga gaaagaagcg atcaaagaag cgtcttcggg cggagtagga
      301 gaatgcggag gagaaggaga acgagctgat ctagtatctc tccacaatcc aatgccaact
      361 gaccaactgg ccatattcgg agcaatttga agccaatttc catcgcctgg cgatcgctcc
      421 attcttggct atatgttttt caccgttacc cggggccatt ttcaaagact cgtcggcaag
      481 ataagattgt gtcactcgct gtctctcttc atttgtcgaa gaatgctgag gaatttcgcg
      541 atgacgtcgg cgagtatttt gaagaatgag aataatttgt atttatacga aaatcagtta
      601 gtggaatttt ctacaaaaac atgttatcta tagataattt tgttgcaaaa tatgttgact
      661 atgacaaaga ttgtatgtat atacctttaa tgtattctca ttttcttatg tatttataat
      721 ggcaatgatg atactgatga tattttaaga tgatgccaga ccaaaaggct tgaatttctg
      781 cgtcttttgc cgaacgcagt gcatgtgcaa ttgttgtttt ttggaatatt caattttcgg
      841 actgtccgct ttgatttcag tttcttggct tattcaaaaa gcaaagtaaa gccaaaaaag
      901 cgagatggca ataccaaatg cggcaaaacg gtagtggaag gaaaggggtg cggggcagcg
      961 gaaggaaggg tggggcgggg cgtggcgggg tctgtggctg ggcgcgacgt caccgacgtt
     1021 ggagccactc ctttgaccat gtgtgcgtgt gtgtattatt cgtgtctcgc cactcgccgg
     1081 ttgttttttt ctttttatgc tgcgctctct ctagcgccat ctcgcttacg catgctcaac
     1141 gcaccgcatg ttgccgtttc cttttatgcg tcattttggc tcgaaatagg caattattta
     1201 aacaaagatt agtcaacgaa aacgctaaaa taaataagtc tacaatatgg ttacttattg
     1261 ccatgtgtgt gcagccaacg atagcaacaa aagcaacaac acaggtggct ttccctcttt
     1321 cactttttgt ttgcaagccg cgtgcgagca agacggcacg accggcaaac gcaattacgc
     1381 tgacaaagag cagacgaagt tttggcgaaa aacatcaagg cgcctgatac gaatgcattt
     1441 gcaataacaa ttgcgatatt taatattgtt tatgaagctg tttgacttca aaacacacaa
     1501 aaaaaaaaat aaaacaaatt atttgaaaga gaattaggaa tcggacgctt atcgttaggg
     1561 taacaacaag aaatgcttac tgagtcacag cctctggaaa actgccgcaa gccagagaga
     1621 gagagaaaaa gagggagagc agcttagacc gcatgtgctt gtgtgtgagg cgtctctctc
     1681 ttcgtctctg ttgcgcaaac gcatagactg cactgaaaaa atcgattacc tattttttat
     1741 gaatgaatat ttgcactatt actattcaaa actattaaga tagcaatcac attcaatagc
     1801 caaatactat accacctgag cgatgcaacg aaatgatcaa tttgagcaaa aatgctgcat
     1861 atttaggacg gcatcattat agaaatgctt cttgctgtgt acttttctct cgtctggcag
     1921 ctgtttcgcc gttattgtta aaaccggctt aagttaggtg tgttttctac gactagtgaa
     1981 tgccctacta gaagatgtgt gttgcacaaa atgtccctgg aataaccaat ttgaagtgca
     2041 gatagcagta aacgtaagct aatatgaata ttatttaact gtaatgtttt aatatcgctg
     2101 gacattacta ataaacccac tataaacaca tgtacatatg tatgttttgg catacaatga
     2161 gtagttgggg aaaaaatgtg taaaagcacc gtgaccatca cagcataaag ataaccagct
     2221 gaagtatcga atatgagtaa cccccaaatt gaatcacatg ccgcaactga taggacccat
     2281 ggaagtacac tcttcatggc gatatacaag acacacacaa gcacgaacac ccagttgcgg
     2341 aggaaattct ccgtaaatga aaacccaatc ggcgaacaat tcatacccat atatggtaaa
     2401 agttttgaac gcgacttgag agcggagagc attgcggctg ataaggtttt agcgctaagc
     2461 gggctttata aaacgggctg cgggaccagt tttcatatca ctaccgtttg agttcttgtg
     2521 ctgtgtggat actcctcccg acacaaagcc gctccatcag ccagcagtcg tctaatccag
     2581 agaccccgga tcggggtacc aacatggacg cacaaacacg acgacgtgag cgtcgcgctg
     2641 agaaacaagc tcaatggaaa gctgcaaacg gtggatctcc tccacatatg gcttacccat
     2701 acgatgttcc agattacgct cctccaagct tgaattcaat ggttgccgat aacatcgatg
     2761 ccggagtggc cattgccgtc gccgatcaat cctcgtcgcc cgtgggcgat aaagttgagc
     2821 tgccggccgg taattatatc ctggtgggcg tggatatcga caccactgga cgtcgtctaa
     2881 tggatgagat tgtccagctg gccgcttata cacccaccga ccacttcgag cagtacatca
     2941 tgccatatat gaatctgaat ccagctgctc gccagcgtca tcaagttcgt gttatttcga
     3001 ttggctttta tcgtatgctg aagtcgatgc agacttataa gatcattaaa tccaagtctg
     3061 agatcgctgc cctcaaggac ttcctcaact ggcttgagca actgaagacc aaggcgggtc
     3121 ccagctcgga cgggattgtg cttatctatc atgaagagcg caagttcatt ccctacatga
     3181 tcctggagtc gttgaagaag tacggcctgt tggagcgctt caccgcgtcg gtgaagtcgt
     3241 ttgcaaacag catcaatctg gccaaggctt ccattggcga tgccaacata aagaactaca
     3301 gtctgcgaaa gctttccaag atcttgtcca cgaccaagga ggaagacgcg gcttgctctg
     3361 cgtccacatc tggatctgga tctggcttgg gatctggatc tagtatggtg tccgattcgg
     3421 tatcgatatc ccccagggac agtaccgtga ccaatggtga cgataagcaa tctagcaaga
     3481 acgccgttca gggtaagcgg gaactgtttg atggcaatgc cagtgttcgt gccaaattgg
     3541 cctttgatgt ggcccttcag ctgagcaact ccgacggcaa gccggaacca aaaagctcgg
     3601 aggcattgga gaatatgttt aatgccatac gtccgtttgc caagttggtg gtgtccgatg
     3661 tcctggagct ggatatccag atcgaaaatt tggagcgtca aaactccttc cgtccggtgt
     3721 tcctgaacta cttcaagacc actctgtacc atcgtgtgcg tgccgtgaag ttccgcattg
     3781 tgctggccga gaatgggttc gatttgaaca cgctaagtgc catctgggca gagaaaaaca
     3841 tcgagggcct ggacatagcc ctgcagtcaa ttggccggct aaagagcaag gacaaggcgg
     3901 agctgctcga gcttctggac agctatttcg atcccaagaa gaccacggtg aagccggttg
     3961 tcaagggtaa cagcaacaat aataacaatt atcgtcgtcg caatcgacgc ggcggccgcc
     4021 aatcggtgaa ggacgctagg ccatccagct ccccatcggc tagcaccgag tttggagcag
     4081 gaggtgacaa gtcgcgcagc gtttcctcgt tgcccgactc tactaccaag actccctcgc
     4141 caaacaagcc gcgtatgcac cgtaagcgca actcgcgtca aagcttgggt gcaaccccca
     4201 atggactcaa ggttgctgct gaaatatcct cctcaggcgt gtctgagctc aacaatagcg
     4261 ctccacctgc cgtaaccata agcccagtcg tggcacaacc ctcaccgaca ccggtggcca
     4321 tcacggcctc caactaatct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     4381 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc
     4441 cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag
     4501 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     4561 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4621 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     4681 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     4741 cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     4801 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     4861 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     4921 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     4981 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     5041 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     5101 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     5161 agatgacgac catcagggac agcttcaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5221 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5281 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5341 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5401 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5461 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5521 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5581 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5641 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     5701 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5761 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5821 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5881 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5941 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6001 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6061 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6121 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6181 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6241 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6301 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6361 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6421 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6481 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6541 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6601 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6661 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6721 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6781 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6841 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6901 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6961 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     7021 ctataaaaat aggcg
//
