LOCUS       sequence-333045-        6205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmZuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6205)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmZuc_B
FEATURES             Location/Qualifiers
     source          1..6205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     53..71
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        135..2644
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2664..2729
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2754..2780
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3590..3631
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3641..3658
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3681..3698)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3731..3865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3755..3774)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3809..3828
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4466..5054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4546..4565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5225..6016,6017..6085))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5848..5867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6086..6190)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
       61 tatcacgagg ccctttcgtc ttcaagaatt aattctatat tctaaaaaca caaatgatac
      121 ttctaaaaaa aaatcatgaa tggcatcaac tctgaatcaa atctttgcag atgcacctac
      181 ttctcatttc cactgtcaca tcatttttcc agatctcgct gcctgttatg tggcccacaa
      241 accaagacac gttttatggc cattaaagct ggctgatcgt cgccaaacac caaatacata
      301 atgaatatgt acacattcga gaaagaagcg atcaaagaag cgtcttcggg cggagtagga
      361 gaatgcggag gagaaggaga acgagctgat ctagtatctc tccacaatcc aatgccaact
      421 gaccaactgg ccatattcgg agcaatttga agccaatttc catcgcctgg cgatcgctcc
      481 attcttggct atatgttttt caccgttacc cggggccatt ttcaaagact cgtcggcaag
      541 ataagattgt gtcactcgct gtctctcttc atttgtcgaa gaatgctgag gaatttcgcg
      601 atgacgtcgg cgagtatttt gaagaatgag aataatttgt atttatacga aaatcagtta
      661 gtggaatttt ctacaaaaac atgttatcta tagataattt tgttgcaaaa tatgttgact
      721 atgacaaaga ttgtatgtat atacctttaa tgtattctca ttttcttatg tatttataat
      781 ggcaatgatg atactgatga tattttaaga tgatgccaga ccaaaaggct tgaatttctg
      841 cgtcttttgc cgaacgcagt gcatgtgcaa ttgttgtttt ttggaatatt caattttcgg
      901 actgtccgct ttgatttcag tttcttggct tattcaaaaa gcaaagtaaa gccaaaaaag
      961 cgagatggca ataccaaatg cggcaaaacg gtagtggaag gaaaggggtg cggggcagcg
     1021 gaaggaaggg tggggcgggg cgtggcgggg tctgtggctg ggcgcgacgt caccgacgtt
     1081 ggagccactc ctttgaccat gtgtgcgtgt gtgtattatt cgtgtctcgc cactcgccgg
     1141 ttgttttttt ctttttatgc tgcgctctct ctagcgccat ctcgcttacg catgctcaac
     1201 gcaccgcatg ttgccgtttc cttttatgcg tcattttggc tcgaaatagg caattattta
     1261 aacaaagatt agtcaacgaa aacgctaaaa taaataagtc tacaatatgg ttacttattg
     1321 ccatgtgtgt gcagccaacg atagcaacaa aagcaacaac acaggtggct ttccctcttt
     1381 cactttttgt ttgcaagccg cgtgcgagca agacggcacg accggcaaac gcaattacgc
     1441 tgacaaagag cagacgaagt tttggcgaaa aacatcaagg cgcctgatac gaatgcattt
     1501 gcaataacaa ttgcgatatt taatattgtt tatgaagctg tttgacttca aaacacacaa
     1561 aaaaaaaaat aaaacaaatt atttgaaaga gaattaggaa tcggacgctt atcgttaggg
     1621 taacaacaag aaatgcttac tgagtcacag cctctggaaa actgccgcaa gccagagaga
     1681 gagagaaaaa gagggagagc agcttagacc gcatgtgctt gtgtgtgagg cgtctctctc
     1741 ttcgtctctg ttgcgcaaac gcatagactg cactgaaaaa atcgattacc tattttttat
     1801 gaatgaatat ttgcactatt actattcaaa actattaaga tagcaatcac attcaatagc
     1861 caaatactat accacctgag cgatgcaacg aaatgatcaa tttgagcaaa aatgctgcat
     1921 atttaggacg gcatcattat agaaatgctt cttgctgtgt acttttctct cgtctggcag
     1981 ctgtttcgcc gttattgtta aaaccggctt aagttaggtg tgttttctac gactagtgaa
     2041 tgccctacta gaagatgtgt gttgcacaaa atgtccctgg aataaccaat ttgaagtgca
     2101 gatagcagta aacgtaagct aatatgaata ttatttaact gtaatgtttt aatatcgctg
     2161 gacattacta ataaacccac tataaacaca tgtacatatg tatgttttgg catacaatga
     2221 gtagttgggg aaaaaatgtg taaaagcacc gtgaccatca cagcataaag ataaccagct
     2281 gaagtatcga atatgagtaa cccccaaatt gaatcacatg ccgcaactga taggacccat
     2341 ggaagtacac tcttcatggc gatatacaag acacacacaa gcacgaacac ccagttgcgg
     2401 aggaaattct ccgtaaatga aaacccaatc ggcgaacaat tcatacccat atatggtaaa
     2461 agttttgaac gcgacttgag agcggagagc attgcggctg ataaggtttt agcgctaagc
     2521 gggctttata aaacgggctg cgggaccagt tttcatatca ctaccgtttg agttcttgtg
     2581 ctgtgtggat actcctcccg acacaaagcc gctccatcag ccagcagtcg tctaatccag
     2641 agaccccgga tcggggtacc aacatggacg cacaaacacg acgacgtgag cgtcgcgctg
     2701 agaaacaagc tcaatggaaa gctgcaaacg gtggatctcc tccacatatg gcttacccat
     2761 acgatgttcc agattacgct cctccaagct tgatgttgat tacccaaata attatgaaac
     2821 aaattcgaga ctatcccatt gtgtccacaa taagcattgc cgtcagcact gtcctggcct
     2881 cagaggtgat ttggaagctg gtgcagtgct cgcgcagcaa gcgagagaag gcaagccgcg
     2941 tgcacgaagt gatcatcttc aatgaactgg gcgagatctg tgcggcggtg catatgcgca
     3001 acagcagcat gggatcccag aaaccacaag tgtcgccctg ctgcaacacg cattgttcgc
     3061 tgcggaatgt ggccaagatc gtagagcaga tcgatcgggc tgtatactcc attgacctgg
     3121 ccatatacac cttcacctcg ctttttttgg cggattcaat aaagcgggcc ctgcagcgcg
     3181 gcgtgattat ccggatcatc agcgacgggg agatggtcta ctccaaaggt tctcagatca
     3241 gtatgctggc acaattggga gttccagttc gcgtccccat taccacgaac ttgatgcaca
     3301 acaaattctg cataatcgac ggcttcgagc gcgtcgagga gattcgtctg ctgaggaagc
     3361 tcaagtttat gcgtccgtgc tatagcatcg tgatcagcgg ctccgtaaac tggacggctc
     3421 ttggactggg aggcaattgg gagaactgca ttatcacagc tgacgacaaa ctcacagcca
     3481 cgtttcaggc ggaattccaa cgaatgtggc gggcttttgc gaagaccgag gggagccaaa
     3541 tccagctcaa gtaggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     3601 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     3661 agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc
     3721 ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     3781 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3841 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     3901 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     3961 tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     4021 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     4081 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     4141 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     4201 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     4261 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     4321 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     4381 ccaggcaggt agatgacgac catcagggac agcttcaagg ccagcaaaag gccaggaacc
     4441 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4501 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4561 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4621 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4681 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4741 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4801 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4861 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     4921 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4981 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5041 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5101 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5161 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5221 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5281 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5341 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5401 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5461 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5521 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5581 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5641 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5701 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5761 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5821 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     5881 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5941 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6001 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6061 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6121 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6181 gggttccgcg cacatttccc cgaaa
//
