LOCUS       sequence-333047-        8005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmTral-R42E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Tritschler F, Eulalio A, Helms S, Schmidt S, Coles M, Weichenrieder 
            O, Izaurralde E, Truffault V
  TITLE     Similar modes of interaction enable Trailer Hitch and EDC3 to 
            associate with DCP1 and Me31B in distinct protein complexes.
  JOURNAL   Mol Cell Biol. 2008 Nov . 28(21):6695-708.
  PUBMED    18765641
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Nov . 28(21):6695-708."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmTral-R42E_B
FEATURES             Location/Qualifiers
     source          1..8005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..60
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        124..2633
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2653..2655,2656..2658,2659..3369)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2653..2655,2656..2658,2659..3369)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2959..2978)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3306..3327
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5379..5420
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5430..5447
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5470..5487)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5520..5654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5544..5563)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5598..5617
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6255..6843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6335..6354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7014..7805,7806..7874))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7637..7656
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7875..7979)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
       61 cctttcgtct tcaagaatta attctatatt ctaaaaacac aaatgatact tctaaaaaaa
      121 aatcatgaat ggcatcaact ctgaatcaaa tctttgcaga tgcacctact tctcatttcc
      181 actgtcacat catttttcca gatctcgctg cctgttatgt ggcccacaaa ccaagacacg
      241 ttttatggcc attaaagctg gctgatcgtc gccaaacacc aaatacataa tgaatatgta
      301 cacattcgag aaagaagcga tcaaagaagc gtcttcgggc ggagtaggag aatgcggagg
      361 agaaggagaa cgagctgatc tagtatctct ccacaatcca atgccaactg accaactggc
      421 catattcgga gcaatttgaa gccaatttcc atcgcctggc gatcgctcca ttcttggcta
      481 tatgtttttc accgttaccc ggggccattt tcaaagactc gtcggcaaga taagattgtg
      541 tcactcgctg tctctcttca tttgtcgaag aatgctgagg aatttcgcga tgacgtcggc
      601 gagtattttg aagaatgaga ataatttgta tttatacgaa aatcagttag tggaattttc
      661 tacaaaaaca tgttatctat agataatttt gttgcaaaat atgttgacta tgacaaagat
      721 tgtatgtata tacctttaat gtattctcat tttcttatgt atttataatg gcaatgatga
      781 tactgatgat attttaagat gatgccagac caaaaggctt gaatttctgc gtcttttgcc
      841 gaacgcagtg catgtgcaat tgttgttttt tggaatattc aattttcgga ctgtccgctt
      901 tgatttcagt ttcttggctt attcaaaaag caaagtaaag ccaaaaaagc gagatggcaa
      961 taccaaatgc ggcaaaacgg tagtggaagg aaaggggtgc ggggcagcgg aaggaagggt
     1021 ggggcggggc gtggcggggt ctgtggctgg gcgcgacgtc accgacgttg gagccactcc
     1081 tttgaccatg tgtgcgtgtg tgtattattc gtgtctcgcc actcgccggt tgtttttttc
     1141 tttttatgct gcgctctctc tagcgccatc tcgcttacgc atgctcaacg caccgcatgt
     1201 tgccgtttcc ttttatgcgt cattttggct cgaaataggc aattatttaa acaaagatta
     1261 gtcaacgaaa acgctaaaat aaataagtct acaatatggt tacttattgc catgtgtgtg
     1321 cagccaacga tagcaacaaa agcaacaaca caggtggctt tccctctttc actttttgtt
     1381 tgcaagccgc gtgcgagcaa gacggcacga ccggcaaacg caattacgct gacaaagagc
     1441 agacgaagtt ttggcgaaaa acatcaaggc gcctgatacg aatgcatttg caataacaat
     1501 tgcgatattt aatattgttt atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata
     1561 aaacaaatta tttgaaagag aattaggaat cggacgctta tcgttagggt aacaacaaga
     1621 aatgcttact gagtcacagc ctctggaaaa ctgccgcaag ccagagagag agagaaaaag
     1681 agggagagca gcttagaccg catgtgcttg tgtgtgaggc gtctctctct tcgtctctgt
     1741 tgcgcaaacg catagactgc actgaaaaaa tcgattacct attttttatg aatgaatatt
     1801 tgcactatta ctattcaaaa ctattaagat agcaatcaca ttcaatagcc aaatactata
     1861 ccacctgagc gatgcaacga aatgatcaat ttgagcaaaa atgctgcata tttaggacgg
     1921 catcattata gaaatgcttc ttgctgtgta cttttctctc gtctggcagc tgtttcgccg
     1981 ttattgttaa aaccggctta agttaggtgt gttttctacg actagtgaat gccctactag
     2041 aagatgtgtg ttgcacaaaa tgtccctgga ataaccaatt tgaagtgcag atagcagtaa
     2101 acgtaagcta atatgaatat tatttaactg taatgtttta atatcgctgg acattactaa
     2161 taaacccact ataaacacat gtacatatgt atgttttggc atacaatgag tagttgggga
     2221 aaaaatgtgt aaaagcaccg tgaccatcac agcataaaga taaccagctg aagtatcgaa
     2281 tatgagtaac ccccaaattg aatcacatgc cgcaactgat aggacccatg gaagtacact
     2341 cttcatggcg atatacaaga cacacacaag cacgaacacc cagttgcgga ggaaattctc
     2401 cgtaaatgaa aacccaatcg gcgaacaatt catacccata tatggtaaaa gttttgaacg
     2461 cgacttgaga gcggagagca ttgcggctga taaggtttta gcgctaagcg ggctttataa
     2521 aacgggctgc gggaccagtt ttcatatcac taccgtttga gttcttgtgc tgtgtggata
     2581 ctcctcccga cacaaagccg ctccatcagc cagcagtcgt ctaatccaga gaccccggat
     2641 cggggtacca tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     2701 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     2761 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     2821 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     2881 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     2941 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3001 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3061 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3121 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     3181 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     3241 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     3301 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     3361 ctgtacaagc caagcttgaa ttctatgagc gggggattac cggagttggg ctccaaaatc
     3421 agcctcattt ccaaggcgga cattcgatac gagggccgcc tgtacacggt ggatccgcag
     3481 gagtgcacca ttgcgctctc gagtgtggag tccttcggta cagaagatcg ggacacccag
     3541 ttccagatag cgccgcaatc gcagatctac gactacatcc tcttccgtgg ctcggacatc
     3601 aaggacattc gcgttgtcaa caaccacacg ctgccgcacc acaatgaccc ggcgatcatg
     3661 caggcgcagc tccagaacgg accgccccag atgccgcagc acttccccat gcccagcggg
     3721 atgagcggtc cgccgcagca gcaacaggtg ccgtcgcagc aaccgcccca gatgccaagc
     3781 ggtggaggca gtggtggtgc tggtggagca ggagctcctg gcggcggcgg acccggctac
     3841 ggcaatggca atccgttcgg caacctcggc ggtcccaatc tggccaacat ggtgggcaat
     3901 gcgggcggat cgcttgcacc cggttccggt gctccgggca gcggaccctt catgcacatc
     3961 ggtggcaatc agcagcaacc gaagccccag cagcagaaac agccaaacat gctggccggt
     4021 gcctcgcgat cgacgacgcc cattagcctg attgtgagtc ccacggcgga gctgactcag
     4081 cagcagcagc tgcaccagca acagaacgct ggccagggtg gcaacggacg cgatgcagga
     4141 cataagcgcc agaaccacca gcagcaacag aatcagcagc atcagcaacg tggcggaccc
     4201 agccacaaca atatgcagca tcagcagcaa cgaggcggcg gcagtggaac ggacttctat
     4261 aaccagcagc gggatcgtcg ggactccgga cgtcaaatgg acaacaacta tagcaacaac
     4321 tacaacaaca ataataataa tcagcgcaat cgcggcggcg gcaacggaat gcaacagcag
     4381 cagcgaggag gaaacggcgg cagcggcggc ggcggtggaa acggaggtgg aaacaacccg
     4441 gcctggaaca tgcatcgcgg caaccagaac tcgaacaaca tgatgaacat gcgcaaccgc
     4501 ggcatgggat cccgcggccc catgcgaccc aatcagggct atcgtccgca gtcggccaat
     4561 aatcagaaca agccgcggaa caagatcaag ttcgagggcg acttcgattt cgagcaggca
     4621 aacaacaagt tcgaggaact gcgctcccaa ctggccaagc tcaaggtggc cgaggatggt
     4681 gcacccaagc cagccaccaa tgcaacggcc gccactgcaa ctgcaaccaa tgagcaggtg
     4741 ggtgagaagg ttgaaggcgt tcacacactg aatggcgaga ccgacaagaa ggatgattct
     4801 ggcaacgaga ccggcgctgg agagcacgag cctgaggagg atgatgttgc tgtgtgctac
     4861 gacaagacca aatcgttctt cgacaacatc tcgtgcgagg ctgcccagga tcgcagcaag
     4921 aacaagaaga acgattggcg ccaggagcgc aagttgaaca cggagacctt cggagtgtcc
     4981 tccacacgac gtggcagtta ccgcggacgc aaccaatact acaacaataa tggcaacggc
     5041 ggcattaact cgggatatgg aggagcgcca ggctacaaca ggaacaatta tcgcatgggt
     5101 ggcggcggcg gaaacttccg aaacaggagc aacaatcgca acaacggcgg cggtcgtggc
     5161 ggaaacggaa tgccaaacat caccaatggc aacacggctg ctgcgctgaa ggcggccaac
     5221 aatgctgctg gccacggatc caatgccacg gactccagtg caccaaatgc cacaaccgcg
     5281 acgacaaagt cgacgtccct cttgccagag cagacgcaac aggtggcggc agtttcacaa
     5341 taggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     5401 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     5461 gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg
     5521 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     5581 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     5641 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     5701 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     5761 agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     5821 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     5881 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     5941 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     6001 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     6061 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     6121 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     6181 gatgacgacc atcagggaca gcttcaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6661 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     6721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7141 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7201 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7321 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7621 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7981 acatttcccc gaaaagtgcc acctg
//
