LOCUS       sequence-333056-        6858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-HKU1 S_nostop
FEATURES             Location/Qualifiers
     source          1..6858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(49..135)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     184..206
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     198..215
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     199..215
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    231..330
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4399..4498)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4515..4534)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4516..4534)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4539..4555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4539..4555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4986..5777
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5870..6458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6359..6378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6612..6629
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6788..6815)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
       61 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
      121 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt
      181 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga
      241 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
      301 taatgccaac tttgtacaaa aaagttggca tgttattaat tatttttatt ttgcctacaa
      361 cattagctgt tataggtgat tttaattgta ctaattttgc tattaatgat ttaaacacca
      421 cagttcctcg cataagtgag tatgttgtgg atgtttctta tggtttgggt acatattata
      481 tacttgatcg tgtttattta aatactacta tattatttac tggttatttc cctaaatctg
      541 gtgccaattt tagggatcta tctttaaaag gtactacata tttgagtact ctttggtatc
      601 agaaaccctt tttatctgat tttaataatg gtattttttc tagagttaag aatactaagt
      661 tgtatgttaa taaaactttg tatagtgagt ttagtactat agttataggt agtgttttta
      721 ttaacaactc ttatactatt gttgttcaac ctcataatgg tgttttggag attacagctt
      781 gtcaatacac tatgtgtgag tatcctcata ctatttgtaa atctaaaggt agttctcgta
      841 atgaatcttg gcattttgat aaatctgaac ctttgtgtct gttcaagaaa aattttactt
      901 ataatgtttc tacagattgg ttgtattttc atttttatca agaacgtggc actttttatg
      961 cttattatgc tgattctggc atgcctacta cttttttatt tagtttgtat cttggtactc
     1021 ttttatctca ttattatgtt ttgcctttga cttgtaatgc tatatcttct aatactgata
     1081 atgagacttt acaatattgg gtcacacctt tgtctaaacg ccaatatctt cttaaatttg
     1141 acaaccgtgg tgttattact aatgctgttg attgttctag tagtttcttt agcgagattc
     1201 aatgtaaaac taaatcttta ttacctaata ctggtgttta tgacttatct ggttttactg
     1261 ttaagcctgt tgcaactgta catcgtcgta ttcctgattt acctgattgt gacattgata
     1321 aatggcttaa caattttaat gtaccctcac ctcttaattg ggaacgtaaa attttttcta
     1381 attgcaactt taatttgagt actttgcttc gtttagttca tactgattct ttttcttgta
     1441 ataattttga tgaatctaag atatatggta gttgttttaa gagtattgtt ttagataaat
     1501 ttgccatacc caactccaga cgatctgatt tgcagttggg cagttctggt tttctgcaat
     1561 cttctaatta taaaattgac actacttcta gttcttgtca attgtattat agtttgcctg
     1621 caattaatgt tactattaat aattataatc cttcttcttg gaatagaagg tatggtttta
     1681 ataattttaa tttgagctct catagtgttg tttactcacg ttattgtttt tctgttaata
     1741 atactttttg tccttgtgct aaaccttctt ttgcttcaag ttgcaagagt cataaaccac
     1801 cttctgcttc ctgtcctatt ggtactaatt atcgttcttg tgagagtact actgtactcg
     1861 accacactga ctggtgtagg tgttcttgtt tacctgatcc tataactgct tatgacccta
     1921 ggtcttgttc tcaaaaaaag tctctggttg gtgttggtga acattgtgca gggttcggtg
     1981 ttgatgaaga aaagtgtggt gtattggatg gatcatataa tgtttcttgt ctttgtagta
     2041 ctgatgcctt tctaggttgg tcttatgaca cttgcgtcag taacaaccgt tgtaatattt
     2101 tttctaattt tattttaaat ggtatcaata gtggtaccac ttgttctaat gatttattgc
     2161 agcctaatac tgaagttttt actgatgttt gtgttgatta cgacctttat ggtattacag
     2221 gacaaggtat ttttaaagaa gtttctgctg tttattataa tagttggcaa aatcttttgt
     2281 atgattctaa tggcaacatt attggtttta aagattttgt tactaataaa acatataata
     2341 ttttcccttg ttatgcagga agagtttctg ctgcttttca tcaaaatgct tcctctttgg
     2401 ctttacttta tcgtaattta aaatgtagct atgttttgaa taatatttct ttaactactc
     2461 agccatattt tgatagttat cttggttgcg tttttaatgc tgataattta actgattatt
     2521 ctgtttcttc ttgtgctctt cgcatgggta gtggtttttg tgttgattat aactcacctt
     2581 cttcttcctc ttcgcgtcgt aaacgtagaa gtatttctgc ttcttatcgt tttgttactt
     2641 ttgaaccctt taatgtcagt tttgttaatg acagtattga gtctgtgggt ggtctttatg
     2701 agatcaaaat tcccactaac tttactatag ttggtcaaga ggaatttatt caaactaatt
     2761 ctcctaaagt tactattgat tgttctttat ttgtctgttc taattatgca gcttgccatg
     2821 acttattgtc agagtatggc actttttgtg ataatattaa tagtatttta gatgaagtta
     2881 atggtttact tgatactact caattgcatg tagctgatac tcttatgcaa ggtgtcacac
     2941 ttagctccaa tcttaatact aatttgcatt ttgatgttga taatattaat tttaaatccc
     3001 tagttggatg tttaggtcca cactgcggtt cttcttctcg ttcttttttt gaagatttat
     3061 tgtttgacaa agttaaactt tcagatgttg gttttgttga agcttataac aattgtactg
     3121 gtggtagtga aattagagat cttctttgtg tacaatcctt taatggtatt aaagttttgc
     3181 ctcctatttt gtctgaatct caaatttctg gttacaccac agccgctact gttgctgcta
     3241 tgtttccacc atggtcagca gcagctggca taccattttc tcttaatgta caatatagaa
     3301 ttaatggttt gggtgttact atggatgttc ttaataaaaa tcaaaagttg atagctactg
     3361 cttttaataa tgctcttctt tctattcaga atggttttag tgctaccaac tctgcacttg
     3421 ctaaaataca aagtgttgtt aattctaatg ctcaagcact taatagtttg ttacagcaat
     3481 tatttaataa atttggtgca attagttctt ctttacaaga aattttatct cgtctcgatg
     3541 ctttagaggc tcaggttcag attgataggc ttattaatgg tcgtttaact gctttaaatg
     3601 cttatgtctc tcaacagctt agtgatattt ctcttgtaaa atttggtgct gctttagcta
     3661 tggagaaggt taatgagtgt gttaaaagtc aatctcctcg tattaatttt tgtggtaatg
     3721 gtaatcatat tttgtcatta gttcaaaatg ctccttatgg tttgttgttt atgcatttta
     3781 gttataaacc tatttctttt aaaactgttt tagtaagtcc tggtttgtgt atatcaggtg
     3841 atgtaggtat tgcacctaaa caagggtatt ttattaaaca taatgatcat tggatgttca
     3901 ctggtagttc ttactattat cctgaaccaa tttcagataa aaatgttgtt tttatgaata
     3961 cttgttctgt taattttact aaagcgcctc ttgtttattt gaatcattct gtaccaaaat
     4021 tgtctgattt tgaatctgag ttatctcatt ggtttaaaaa tcaaacatcc attgcgccta
     4081 atttgacttt aaatcttcat actattaatg ctactttttt agatttgtat tatgagatga
     4141 atcttattca agagtctatt aagtctttga ataatagtta tatcaatctt aaagatatag
     4201 gtacatatga aatgtatgta aaatggcctt ggtatgtttg gctactaatt tctttttcat
     4261 ttataatatt ccttgtattg ctctttttta tatgttgttg tactggttgt ggttctgcat
     4321 gttttagtaa atgtcataat tgttgtgatg agtatggtgg tcatcatgat tttgttatca
     4381 aaacatctca tgatgattac ccaactttct tgtacaaagt tggcattata agaaagcatt
     4441 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     4501 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     4561 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     4621 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     4681 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     4741 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     4801 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     4861 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     4921 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     4981 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     5041 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     5101 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     5161 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     5221 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     5281 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     5341 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     5401 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     5461 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     5521 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     5581 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     5641 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     5701 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     5761 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     5821 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5881 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6001 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6241 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6481 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6541 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6601 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6661 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6721 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     6781 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     6841 tggcgggcgt cctgcccg
//
