LOCUS       sequence-333087-       12335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged CAD with mutation of reported S6K 
            phosphorylation site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD S1859A
FEATURES             Location/Qualifiers
     source          1..12335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(128..145)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     355..374
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             548..943
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1114..1583
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1713..1730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1884..2472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1964..1983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2636..3427,3428..3496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3259..3278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3497..3601)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3631..4224
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4287..4644
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4703..5119
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5049..5071
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5087..5103
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5129..5503
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5441..5457
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      5530..5539
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12207..12230
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(12296..12335,1..535,536..538,539..547)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
       61 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
      121 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
      181 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
      241 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
      301 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
      361 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
      421 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
      481 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
      541 cacaaccatg gttcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
      601 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
      661 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
      721 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
      781 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
      841 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
      901 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagtcgacg ataaaataaa
      961 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     1021 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
     1081 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
     1141 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
     1201 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     1261 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     1321 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     1381 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     1441 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     1501 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     1561 tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc
     1621 ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg cgcggggaga
     1681 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1741 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1801 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2281 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2581 ttgcggccgg ccgcaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2641 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2701 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2761 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2821 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2881 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2941 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3001 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3061 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3121 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3181 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3241 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3301 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3361 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3421 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3481 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3541 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3601 gcacatttcc ccgaaaagtg ccacctgcat aatgaaagac cccacctgta ggtttggcaa
     3661 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagaaaa
     3721 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     3781 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc
     3841 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     3901 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     3961 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     4021 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg
     4081 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
     4141 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     4201 acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag
     4261 ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat
     4321 tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc
     4381 tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg
     4441 agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga
     4501 tcgttttgga ctctttggtg caccccccta ataggaggga tatgtggttc tggtaggaga
     4561 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag
     4621 ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     4681 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     4741 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     4801 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     4861 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     4921 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     4981 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     5041 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     5101 cactccttct ctaggcgccc ccatatggcc atatgagatc ttatatgggg cacccccgcc
     5161 ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc
     5221 tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta
     5281 ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg
     5341 ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct
     5401 gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca
     5461 cgtgaaggct gccgaccccg ggggtggacc atcctctaga ctgccggatc tagctagtta
     5521 attaaggatc catggcggcc ctagtgttgg aggacgggtc ggtcctgcgg ggccagccct
     5581 ttggggccgc cgtgtcgact gccggggaag tggtgtttca aaccggcatg gtcggctacc
     5641 ccgaggccct cactgatccc tcctacaagg cacagatctt agtgctcacc tatcctctga
     5701 tcggcaacta tggcatcccc ccagatgaaa tggatgagtt cggtctctgc aagtggtttg
     5761 aatcctcggg catccacgta gcagcactgg tagtgggaga gtgctgtcct actcccagcc
     5821 actggagtgc cacccgcacc ctgcatgagt ggctgcagca gcatggcatc cctggcttgc
     5881 aaggagtaga cactcgggag ctgaccaaga agttgcggga acaggggtct ctgctgggga
     5941 agctggtcca gaatggaaca gaaccttcaa gtctgccatt cttggacccc aatgcccgcc
     6001 ccctggtacc agaggtctcc attaagactc cacgggtatt caatacaggg ggtgcccctc
     6061 ggatccttgc tttggactgt ggcctcaagt ataatcagat ccgatgcctc tgccagcgtg
     6121 gggctgaggt cactgtggta ccctgggacc atgcactaga cagccaagag tatgagggtc
     6181 tcttcttaag taatgggcct ggtgaccctg cctcctatcc cagtgtcgta tccacactga
     6241 gccgtgtttt atctgagcct aatccccgac ctgtctttgg gatctgcctg ggacaccagc
     6301 tattggcctt agccattggg gccaagactt acaagatgag atatgggaac cgaggccata
     6361 accagccctg cttgttggtg ggctctgggc gctgctttct gacatcccag aaccatgggt
     6421 ttgctgtgga gacagactca ctgccagcag actgggctcc tctcttcacc aacgccaatg
     6481 atggttccaa tgaaggcatt gtgcacaaca gcttgccttt cttcagtgtc cagtttcacc
     6541 cagagcacca agctggccct tcagatatgg aactgctttt cgatatcttt ctggaaactg
     6601 tgaaagaggc cacagctggg aaccctgggg gccagacagt tagagagcgg ctgactgagc
     6661 gcctctgtcc ccctgggatt cccactcccg gctctggact tccaccacca cgaaaggttc
     6721 tgatcctggg ctcagggggc ctctccattg gccaagctgg agaatttgac tactcgggct
     6781 ctcaggcaat taaggccctg aaggaggaaa acatccagac gttgctgatc aaccccaata
     6841 ttgccacagt gcagacctcc caggggctgg ccgacaaggt ctattttctt cccataacac
     6901 ctcattatgt aacccaggtg atacgtaatg aacgccccga tggtgtgtta ctgacttttg
     6961 ggggccagac tgctctgaac tgtggtgtgg agctgaccaa ggccggggtg ctggctcggt
     7021 atggggtccg ggtcctgggc acaccagtgg agaccattga gctgaccgag gatcgacggg
     7081 cctttgctgc cagaatggca gagatcggag agcatgtggc cccgagcgag gcagcaaatt
     7141 ctcttgaaca ggcccaggca gccgctgaac ggctggggta ccctgtgcta gtgcgtgcag
     7201 cctttgccct gggtggcctg ggctctggct ttgcctctaa cagggaggag ctctctgctc
     7261 tcgtggcccc agcttttgcc cataccagcc aagtgctagt agacaagtct ctgaagggat
     7321 ggaaggagat tgagtacgag gtggtgagag acgcctatgg caactgtgtc acggtgtgta
     7381 acatggagaa cttggaccca ctgggcatcc acactggtga gtccatagtg gtggccccta
     7441 gccagacact gaatgacagg gagtatcagc tcctgaggca gacagctatc aaggtgaccc
     7501 agcacctggg aattgttggg gagtgcaatg tgcagtatgc cttgaaccct gagtctgagc
     7561 agtattacat cattgaagtg aatgccaggc tctctcgcag ctctgccctg gccagtaagg
     7621 ccacaggtta tccactggct tatgtggcag ccaagctagc attgggcatc cctttgcctg
     7681 agctcaggaa ctctgtgaca gggggtacag cagcctttga acccagcgtg gattattgtg
     7741 tggtgaagat tcctcgatgg gaccttagca agttcctgcg agtcagcaca aagattggga
     7801 gctgcatgaa gagcgttggt gaagtcatgg gcattgggcg ttcatttgag gaggccttcc
     7861 agaaggccct gcgcatggtg gatgagaact gtgtgggctt tgatcacaca gtgaaaccag
     7921 tcagcgatat ggagttggag actccaacag ataagcggat ttttgtggtg gcagctgctt
     7981 tgtgggctgg ttattcagtg gaccgcctgt atgagctcac acgcatcgac cgctggttcc
     8041 tgcaccgaat gaagcgtatc atcgcacatg cccagctgct agaacaacac cgtggacagc
     8101 ctttgccgcc agacctgctg caacaggcca agtgtcttgg cttctcagac aaacagattg
     8161 cccttgcagt tctgagcaca gagctggctg ttcgcaagct gcgtcaggaa ctggggatct
     8221 gtccagcagt gaaacagatt gacacagttg cagctgagtg gccagcccag acaaattacc
     8281 tatacctaac gtattggggc accacccatg acctcacctt tcgaacacct catgtcctag
     8341 tccttggctc tggcgtctac cgtattggct ctagcgttga atttgactgg tgtgctgtag
     8401 gctgcatcca gcagctccga aagatgggat ataagaccat catggtgaac tataacccag
     8461 agacagtcag caccgactat gacatgtgtg atcgactcta ctttgatgag atctcttttg
     8521 aggtggtgat ggacatctat gagctcgaga accctgaagg tgtgatccta tccatgggtg
     8581 gacagctgcc caacaacatg gccatggcgt tgcatcggca gcagtgccgg gtgctgggca
     8641 cctcccctga agccattgac tcggctgaga accgtttcaa gttttcccgg ctccttgaca
     8701 ccattggtat cagccagcct cagtggaggg agctcagtga cctcgagtct gctcgccaat
     8761 tctgccagac cgtggggtac ccctgtgtgg tgcgcccctc ctatgtgctg agcggtgctg
     8821 ctatgaatgt ggcctacacg gatggagacc tggagcgctt cctgagcagc gcagcagccg
     8881 tctccaaaga gcatcccgtg gtcatctcca agttcatcca ggaggctaag gagattgacg
     8941 tggatgccgt ggcctctgat ggtgtggtgg cagccatcgc catctctgag catgtggaga
     9001 atgcaggtgt gcattcaggt gatgcgacgc tggtgacccc cccacaagat atcactgcca
     9061 aaaccctgga gcggatcaaa gccattgtgc atgctgtggg ccaggagcta caggtcacag
     9121 gacccttcaa tctgcagctc attgccaagg atgaccagct gaaagttatt gaatgcaacg
     9181 tacgtgtctc tcgctccttc cccttcgttt ccaagacact gggtgtggac ctagtagcct
     9241 tggccacgcg ggtcatcatg ggggaagaag tggaacctgt ggggctaatg actggttctg
     9301 gagttgtggg agtaaaggtg cctcagttct ccttctcccg cttggcgggt gctgacgtgg
     9361 tgttgggtgt ggaaatgacc agtactgggg aggtggccgg ctttggggag agccgctgtg
     9421 aggcatacct caaggccatg ctaagcactg gctttaagat ccccaagaag aatatcctgc
     9481 tgaccattgg cagctataag aacaaaagcg agctgctccc aactgtgcgg ctactggaga
     9541 gcctgggcta cagcctctat gccagtctcg gcacagctga cttctacact gagcatggcg
     9601 tcaaggtaac agctgtggac tggcactttg aggaggctgt ggatggtgag tgcccaccac
     9661 agcggagcat cctggagcag ctagctgaga aaaactttga gctggtgatt aacctgtcaa
     9721 tgcgtggagc tgggggccgg cgtctctctt cctttgtcac caagggctac cgcacccgac
     9781 gcttggccgc tgacttctcc gtgcccctaa tcatcgatat caagtgcacc aaactctttg
     9841 tggaggccct aggccagatc gggccagccc ctcctttgaa ggtgcatgtt gactgtatga
     9901 cctcccaaaa gcttgtgcga ctgccgggat tgattgatgt ccatgtgcac ctgcgggaac
     9961 caggtgggac acataaggag gactttgctt caggcacagc cgctgccctg gctgggggta
    10021 tcaccatggt gtgtgccatg cctaataccc ggccccccat cattgacgcc cctgctctgg
    10081 ccctggccca gaagctggca gaggctggcg cccggtgcga ctttgcgcta ttccttgggg
    10141 cctcgtctga aaatgcagga accttgggca ccgtggccgg gtctgcagcc gggctgaagc
    10201 tttacctcaa tgagaccttc tctgagctgc ggctggacag cgtggtccag tggatggagc
    10261 atttcgagac atggccctcc cacctcccca ttgtggctca cgcggagcag caaaccgtgg
    10321 ctgctgtcct catggtggct cagctcactc agcgctcagt gcacatatgt cacgtggcac
    10381 ggaaggagga gatcctgcta attaaagctg caaaggcacg gggcttgcca gtgacctgcg
    10441 aggtggctcc ccaccacctg ttcctaagcc atgatgacct ggagcgcctg gggcctggga
    10501 agggggaggt ccggcctgag cttggctccc gccaggatgt ggaagccctg tgggagaaca
    10561 tggctgtcat cgactgcttt gcctcagacc atgctcccca taccttggag gagaagtgtg
    10621 ggtccaggcc cccacctggg ttcccagggt tagagaccat gctgccacta ctcctgacgg
    10681 ctgtaagcga gggccggctc agcctggacg acctgctgca gcgattgcac cacaatcctc
    10741 ggcgcatctt tcacctgcct ccgcaggagg acacctatgt ggaggtggat ctggagcatg
    10801 agtggacaat tcccagccac atgcccttct ccaaggccca ctggacacct tttgaagggc
    10861 agaaagtgaa gggcaccgtc cgccgtgtgg tcctgcgagg ggaggttgcc tatatcgatg
    10921 ggcaggttct ggtacccccg ggctatggac aggatgtacg gaagtggcca cagggggctg
    10981 ttcctcagct cccaccctca gcccctgcca ctagtgagat gaccacgaca cctgaaagac
    11041 cccgccgtgg catcccaggg cttcctgatg gccgcttcca tctgccgccc cgaatccatc
    11101 gagccgccga cccaggtttg ccagctgagg agccaaagga gaagtcctct cggaaggtag
    11161 ccgagccaga gctgatggga acccctgatg gcacctgcta ccctccacca ccagtaccga
    11221 gacaggcatc tccccagaac ctggggaccc ctggcttgct gcacccccag acctcacccc
    11281 tgctgcactc attagtgggc caacatatcc tgtccgtcca gcagttcacc aaggatcaga
    11341 tgtctcacct gttcaatgtg gcacacacac tgcgtatgat ggtgcagaag gagcggagcc
    11401 tcgacatcct gaaggggaag gtcatggcct ccatgttcta tgaagtgagc acacggacca
    11461 gcagctcctt tgcagcagcc atggcccggc tgggaggtgc tgtgctcagc ttctcggaag
    11521 ccacatcgtc cgtccagaag ggcgaatccc tggctgactc cgtgcagacc atgagctgct
    11581 atgccgacgt cgtcgtgctc cggcaccccc agcctggagc agtggagctg gccgccaagc
    11641 actgccggag gccagtgatc aatgctgggg atggggtcgg agagcacccc acccaggccc
    11701 tgctggacat cttcaccatc cgtgaggagc tgggaactgt caatggcatg acgatcacga
    11761 tggtgggtga cctgaagcac ggacgcacag tacattccct ggcctgcctg ctcacccagt
    11821 atcgtgtcag cctgcgctac gtggcacctc ccagcctgcg catgccaccc actgtgcggg
    11881 ccttcgtggc ctcccgcggc accaagcagg aggaattcga gagcattgag gaggcgctgc
    11941 ctgacactga tgtgctctac atgactcgaa tccagaagga acgatttggc tctacccagg
    12001 agtacgaagc ttgctttggt cagttcatcc tcactcccca catcatgacc cgggccaaga
    12061 agaagatggt ggtgatgcac ccgatgcccc gtgtcaacga gataagcgtg gaagtggact
    12121 cggatccccg cgcagcctac ttccgccagg ctgagaacgg catgtacatc cgcatggctc
    12181 tgttagccac cgtgctgggc cgtttcgact acaaagacga tgacgacaag taggaattcc
    12241 tgcaggcctc gagggccggc gcgccgcggc cgctacgtaa attccgcccc ccccccccct
    12301 ctccctcccc cccccctaac gttactggcc gaagc
//
