LOCUS       sequence-333093-       11943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged CAD with substitution of allosteric
            domain F1308 - C1455 with a (GGGS)X3 linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11943)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 11943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD delta Allosteric Domain
FEATURES             Location/Qualifiers
     source          1..11943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(128..145)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     355..374
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             548..943
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1114..1583
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1713..1730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1884..2472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1964..1983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2643..3434,3435..3503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3266..3285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3504..3608)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3638..4231
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4294..4651
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4710..5126
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5056..5078
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5094..5110
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5136..5510
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5448..5464
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      5537..5546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11815..11838
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(11904..11943,1..535,536..538,539..547)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
       61 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
      121 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
      181 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
      241 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
      301 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
      361 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
      421 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
      481 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
      541 cacaaccatg gttcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
      601 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
      661 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
      721 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
      781 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
      841 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
      901 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagtcgacg ataaaataaa
      961 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     1021 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
     1081 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
     1141 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
     1201 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     1261 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     1321 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     1381 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     1441 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     1501 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     1561 tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc
     1621 ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg cgcggggaga
     1681 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1741 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1801 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2281 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3241 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3601 gttccgcgca catttccccg aaaagtgcca cctgcataat gaaagacccc acctgtaggt
     3661 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     3721 tagaaaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga
     3781 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
     3841 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     3901 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
     3961 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     4021 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     4081 ctcggcgcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     4141 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     4201 attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc
     4261 tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt atctgtgtct
     4321 gtccgattgt ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa
     4381 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttcggaacac ccggccgcaa
     4441 ccctgggaga cgtcccaggg acttcggggg ccgtttttgt ggcccgacct gagtccaaaa
     4501 atcccgatcg ttttggactc tttggtgcac ccccctaata ggagggatat gtggttctgg
     4561 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttggg
     4621 accgaagccg cgccgcgcgt cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga
     4681 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
     4741 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     4801 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     4861 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     4921 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     4981 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     5041 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     5101 cagccctcac tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac
     5161 ccccgcccct tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc
     5221 tccaagctca cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg
     5281 cagcctacca agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca
     5341 cagtgtgggt ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca
     5401 cagtcctgct gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg
     5461 ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctag
     5521 ctagttaatt aaggatccat ggcggcccta gtgttggagg acgggtcggt cctgcggggc
     5581 cagccctttg gggccgccgt gtcgactgcc ggggaagtgg tgtttcaaac cggcatggtc
     5641 ggctaccccg aggccctcac tgatccctcc tacaaggcac agatcttagt gctcacctat
     5701 cctctgatcg gcaactatgg catcccccca gatgaaatgg atgagttcgg tctctgcaag
     5761 tggtttgaat cctcgggcat ccacgtagca gcactggtag tgggagagtg ctgtcctact
     5821 cccagccact ggagtgccac ccgcaccctg catgagtggc tgcagcagca tggcatccct
     5881 ggcttgcaag gagtagacac tcgggagctg accaagaagt tgcgggaaca ggggtctctg
     5941 ctggggaagc tggtccagaa tggaacagaa ccttcaagtc tgccattctt ggaccccaat
     6001 gcccgccccc tggtaccaga ggtctccatt aagactccac gggtattcaa tacagggggt
     6061 gcccctcgga tccttgcttt ggactgtggc ctcaagtata atcagatccg atgcctctgc
     6121 cagcgtgggg ctgaggtcac tgtggtaccc tgggaccatg cactagacag ccaagagtat
     6181 gagggtctct tcttaagtaa tgggcctggt gaccctgcct cctatcccag tgtcgtatcc
     6241 acactgagcc gtgttttatc tgagcctaat ccccgacctg tctttgggat ctgcctggga
     6301 caccagctat tggccttagc cattggggcc aagacttaca agatgagata tgggaaccga
     6361 ggccataacc agccctgctt gttggtgggc tctgggcgct gctttctgac atcccagaac
     6421 catgggtttg ctgtggagac agactcactg ccagcagact gggctcctct cttcaccaac
     6481 gccaatgatg gttccaatga aggcattgtg cacaacagct tgcctttctt cagtgtccag
     6541 tttcacccag agcaccaagc tggcccttca gatatggaac tgcttttcga tatctttctg
     6601 gaaactgtga aagaggccac agctgggaac cctgggggcc agacagttag agagcggctg
     6661 actgagcgcc tctgtccccc tgggattccc actcccggct ctggacttcc accaccacga
     6721 aaggttctga tcctgggctc agggggcctc tccattggcc aagctggaga atttgactac
     6781 tcgggctctc aggcaattaa ggccctgaag gaggaaaaca tccagacgtt gctgatcaac
     6841 cccaatattg ccacagtgca gacctcccag gggctggccg acaaggtcta ttttcttccc
     6901 ataacacctc attatgtaac ccaggtgata cgtaatgaac gccccgatgg tgtgttactg
     6961 acttttgggg gccagactgc tctgaactgt ggtgtggagc tgaccaaggc cggggtgctg
     7021 gctcggtatg gggtccgggt cctgggcaca ccagtggaga ccattgagct gaccgaggat
     7081 cgacgggcct ttgctgccag aatggcagag atcggagagc atgtggcccc gagcgaggca
     7141 gcaaattctc ttgaacaggc ccaggcagcc gctgaacggc tggggtaccc tgtgctagtg
     7201 cgtgcagcct ttgccctggg tggcctgggc tctggctttg cctctaacag ggaggagctc
     7261 tctgctctcg tggccccagc ttttgcccat accagccaag tgctagtaga caagtctctg
     7321 aagggatgga aggagattga gtacgaggtg gtgagagacg cctatggcaa ctgtgtcacg
     7381 gtgtgtaaca tggagaactt ggacccactg ggcatccaca ctggtgagtc catagtggtg
     7441 gcccctagcc agacactgaa tgacagggag tatcagctcc tgaggcagac agctatcaag
     7501 gtgacccagc acctgggaat tgttggggag tgcaatgtgc agtatgcctt gaaccctgag
     7561 tctgagcagt attacatcat tgaagtgaat gccaggctct ctcgcagctc tgccctggcc
     7621 agtaaggcca caggttatcc actggcttat gtggcagcca agctagcatt gggcatccct
     7681 ttgcctgagc tcaggaactc tgtgacaggg ggtacagcag cctttgaacc cagcgtggat
     7741 tattgtgtgg tgaagattcc tcgatgggac cttagcaagt tcctgcgagt cagcacaaag
     7801 attgggagct gcatgaagag cgttggtgaa gtcatgggca ttgggcgttc atttgaggag
     7861 gccttccaga aggccctgcg catggtggat gagaactgtg tgggctttga tcacacagtg
     7921 aaaccagtca gcgatatgga gttggagact ccaacagata agcggatttt tgtggtggca
     7981 gctgctttgt gggctggtta ttcagtggac cgcctgtatg agctcacacg catcgaccgc
     8041 tggttcctgc accgaatgaa gcgtatcatc gcacatgccc agctgctaga acaacaccgt
     8101 ggacagcctt tgccgccaga cctgctgcaa caggccaagt gtcttggctt ctcagacaaa
     8161 cagattgccc ttgcagttct gagcacagag ctggctgttc gcaagctgcg tcaggaactg
     8221 gggatctgtc cagcagtgaa acagattgac acagttgcag ctgagtggcc agcccagaca
     8281 aattacctat acctaacgta ttggggcacc acccatgacc tcacctttcg aacacctcat
     8341 gtcctagtcc ttggctctgg cgtctaccgt attggctcta gcgttgaatt tgactggtgt
     8401 gctgtaggct gcatccagca gctccgaaag atgggatata agaccatcat ggtgaactat
     8461 aacccagaga cagtcagcac cgactatgac atgtgtgatc gactctactt tgatgagatc
     8521 tcttttgagg tggtgatgga catctatgag ctcgagaacc ctgaaggtgt gatcctatcc
     8581 atgggtggac agctgcccaa caacatggcc atggcgttgc atcggcagca gtgccgggtg
     8641 ctgggcacct cccctgaagc cattgactcg gctgagaacc gtttcaagtt ttcccggctc
     8701 cttgacacca ttggtatcag ccagcctcag tggagggagc tcagtgacct cgagtctgct
     8761 cgccaattct gccagaccgt ggggtacccc tgtgtggtgc gcccctccta tgtgctgagc
     8821 ggtgctgcta tgaatgtggc ctacacggat ggagacctgg agcgcttcct gagcagcgca
     8881 gcagccgtct ccaaagagca tcccgtggtc atctccaagt tcatccagga ggctaaggag
     8941 attgacgtgg atgccgtggc ctctgatggt gtggtggcag ccatcgccat ctctgagcat
     9001 gtggagaatg caggtgtgca ttcaggtgat gcgacgctgg tgaccccccc acaagatatc
     9061 actgccaaaa ccctggagcg gatcaaagcc attgtgcatg ctgtgggcca ggagctacag
     9121 gtcacaggac ccttcaatct gcagctcatt gccaaggatg accagctgaa agttattgaa
     9181 tgcaacgtac gtgtctctcg ctccttcccc ttcgtttcca agacactggg tgtggaccta
     9241 gtagccttgg ccacgcgggt catcatgggg gaagaagtgg aacctgtggg gctaatgact
     9301 ggttctggag ttgtgggagt aaaggtgcct cagttctcct tctcccgctt ggcgggtgct
     9361 gacgtggtgt tgggtgtgga aatgaccagt actggggagg tggccggctt tggggagagc
     9421 cgctgtgagg catacctcaa ggccatgcta agcactggcg gcggtggtgg cagcgggggc
     9481 gggggcagcg gagggggtgg ctcaatgacc tcccaaaagc ttgtgcgact gccgggattg
     9541 attgatgtcc atgtgcacct gcgggaacca ggtgggacac ataaggagga ctttgcttca
     9601 ggcacagccg ctgccctggc tgggggtatc accatggtgt gtgccatgcc taatacccgg
     9661 ccccccatca ttgacgcccc tgctctggcc ctggcccaga agctggcaga ggctggcgcc
     9721 cggtgcgact ttgcgctatt ccttggggcc tcgtctgaaa atgcaggaac cttgggcacc
     9781 gtggccgggt ctgcagccgg gctgaagctt tacctcaatg agaccttctc tgagctgcgg
     9841 ctggacagcg tggtccagtg gatggagcat ttcgagacat ggccctccca cctccccatt
     9901 gtggctcacg cggagcagca aaccgtggct gctgtcctca tggtggctca gctcactcag
     9961 cgctcagtgc acatatgtca cgtggcacgg aaggaggaga tcctgctaat taaagctgca
    10021 aaggcacggg gcttgccagt gacctgcgag gtggctcccc accacctgtt cctaagccat
    10081 gatgacctgg agcgcctggg gcctgggaag ggggaggtcc ggcctgagct tggctcccgc
    10141 caggatgtgg aagccctgtg ggagaacatg gctgtcatcg actgctttgc ctcagaccat
    10201 gctccccata ccttggagga gaagtgtggg tccaggcccc cacctgggtt cccagggtta
    10261 gagaccatgc tgccactact cctgacggct gtaagcgagg gccggctcag cctggacgac
    10321 ctgctgcagc gattgcacca caatcctcgg cgcatctttc acctgcctcc gcaggaggac
    10381 acctatgtgg aggtggatct ggagcatgag tggacaattc ccagccacat gcccttctcc
    10441 aaggcccact ggacaccttt tgaagggcag aaagtgaagg gcaccgtccg ccgtgtggtc
    10501 ctgcgagggg aggttgccta tatcgatggg caggttctgg tacccccggg ctatggacag
    10561 gatgtacgga agtggccaca gggggctgtt cctcagctcc caccctcagc ccctgccact
    10621 agtgagatga ccacgacacc tgaaagaccc cgccgtggca tcccagggct tcctgatggc
    10681 cgcttccatc tgccgccccg aatccatcga gcctccgacc caggtttgcc agctgaggag
    10741 ccaaaggaga agtcctctcg gaaggtagcc gagccagagc tgatgggaac ccctgatggc
    10801 acctgctacc ctccaccacc agtaccgaga caggcatctc cccagaacct ggggacccct
    10861 ggcttgctgc acccccagac ctcacccctg ctgcactcat tagtgggcca acatatcctg
    10921 tccgtccagc agttcaccaa ggatcagatg tctcacctgt tcaatgtggc acacacactg
    10981 cgtatgatgg tgcagaagga gcggagcctc gacatcctga aggggaaggt catggcctcc
    11041 atgttctatg aagtgagcac acggaccagc agctcctttg cagcagccat ggcccggctg
    11101 ggaggtgctg tgctcagctt ctcggaagcc acatcgtccg tccagaaggg cgaatccctg
    11161 gctgactccg tgcagaccat gagctgctat gccgacgtcg tcgtgctccg gcacccccag
    11221 cctggagcag tggagctggc cgccaagcac tgccggaggc cagtgatcaa tgctggggat
    11281 ggggtcggag agcaccccac ccaggccctg ctggacatct tcaccatccg tgaggagctg
    11341 ggaactgtca atggcatgac gatcacgatg gtgggtgacc tgaagcacgg acgcacagta
    11401 cattccctgg cctgcctgct cacccagtat cgtgtcagcc tgcgctacgt ggcacctccc
    11461 agcctgcgca tgccacccac tgtgcgggcc ttcgtggcct cccgcggcac caagcaggag
    11521 gaattcgaga gcattgagga ggcgctgcct gacactgatg tgctctacat gactcgaatc
    11581 cagaaggaac gatttggctc tacccaggag tacgaagctt gctttggtca gttcatcctc
    11641 actccccaca tcatgacccg ggccaagaag aagatggtgg tgatgcaccc gatgccccgt
    11701 gtcaacgaga taagcgtgga agtggactcg gatccccgcg cagcctactt ccgccaggct
    11761 gagaacggca tgtacatccg catggctctg ttagccaccg tgctgggccg tttcgactac
    11821 aaagacgatg acgacaagta ggaattcctg caggcctcga gggccggcgc gccgcggccg
    11881 ctacgtaaat tccgcccccc ccccccctct ccctcccccc cccctaacgt tactggccga
    11941 agc
//
