LOCUS       sequence-333095-        8180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ISCU iron-sulfur cluster trapping mutant (dominant 
            negative).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Terzi EM, Sviderskiy VO, Alvarez SW, Whiten GC, Possemato R
  TITLE     Iron-sulfur cluster deficiency can be sensed by IRP2 and regulates 
            iron homeostasis and sensitivity to ferroptosis independent of IRP1 
            and FBXL5.
  JOURNAL   Sci Adv. 2021 May 26;7(22). pii: 7/22/eabg4302. doi: 
            10.1126/sciadv.abg4302. Print 2021 May.
  PUBMED    34039609
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-05-26"; volume: "7(22). pii: 7/22/eabg4302. doi"; pages:
            " 10.1126/sciadv.abg4302. Print 2021 May"
COMMENT     Sequence Label: pCW57.1 ISCU  D71A
FEATURES             Location/Qualifiers
     source          1..8180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..46
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        645..1155
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1074..1092
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1165..1761
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1165..1184)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1661..1681
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1762..1815
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1816..2433,2434..2439,2440..2475,2476..2478,2479..2514,
                     2515..2517,2518..2553,2554..2562)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(2602..2622)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3020..3031)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3236..3469
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3541..3662
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3578..3597)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3632..3651
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3702..3837
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3837..3908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3909..3977,3978..4769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4127..4146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4940..5528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5429..5448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5682..5699
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5816..5837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5852..5857,5858..5875,5876..5882)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5890..5906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5895..5917
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5914..5930
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5914..5930
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5949..5969
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5951..5969
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5997..6223
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6224..6404
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6451..6576
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7069..7302
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7487..7531
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7744..7861
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(7915..8180,1..49)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    7923..7941
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7959..7977
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7994..8012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8030..8048
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8066..8084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8101..8119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8137..8155
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagaattgg
       61 ctagcaccgg tagtgcttga ccagcggcgc gagttgatcg aagagtgctt caattggcgg
      121 ccgcttattt cttctcagct tcaccttttt tcggttcctg cttcaactta taatctgcca
      181 gcgcggcttt tattgcatct tctgccagca ttgagcaatg caacttgacc ggaggaagac
      241 acaattcctt ggcgatgtcg gtatttttta tagtcagggc ttcctcgacc gttttcccct
      301 tgacccattc cgtcgccaga ctggaactgg cgatagctga cccgcaaccg aaagtcttga
      361 atctcgcgtc tactattttt cctttctcat caacctgtat ttgcaatttc ataacggccc
      421 cgcaagctgg tgccccgacc aacccagtgc ccacgttttt agacgtcttg tccaatgacc
      481 ctacgttgcg aggattttca tagtggtcta ctactttctt gtgataaagt ctagctggag
      541 cgctcagctc gcgagcagga aggcggggac tgcggaggag caacgcgcta gccgctcgcc
      601 taaggcgaaa tgcccctgct gctgccatgg tggaattcgg atccggggtt ggggttgcgc
      661 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
      721 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
      781 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
      841 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
      901 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
      961 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     1021 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     1081 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     1141 gacctctctc cccagcaatt caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     1201 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     1261 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     1321 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     1381 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     1441 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     1501 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     1561 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     1621 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     1681 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     1741 atgacccgca agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa
     1801 gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg agctctggaa
     1861 ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     1921 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     1981 ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg
     2041 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
     2101 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     2161 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     2221 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
     2281 gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca agcaattgag
     2341 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
     2401 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
     2461 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
     2521 gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc
     2581 aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2641 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2701 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2761 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2821 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2881 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2941 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     3001 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3061 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3121 ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca
     3181 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     3241 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     3301 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     3361 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     3421 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     3481 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     3541 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3601 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3661 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     3721 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3781 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt
     3841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4081 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4141 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4201 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4321 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4741 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4801 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5581 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5641 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5701 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5761 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     5821 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     5881 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     5941 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     6001 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     6061 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     6121 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     6181 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     6241 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6301 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6361 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     6421 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     6481 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     6541 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     6601 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     6661 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     6721 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     6781 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     6841 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     6901 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     6961 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     7021 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     7081 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     7141 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     7201 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     7261 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     7321 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     7381 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     7441 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     7501 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     7561 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     7621 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     7681 ccacctccca accccgaggg gacaattctc gacctcgaga caaatggcag tattcatcca
     7741 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     7801 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     7861 tcgggtttat tacagggaca gcagagatcc actttggccg cgaatcgata tgtcgagttt
     7921 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     7981 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
     8041 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     8101 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
     8161 tgtcgaggta ggcgtgtacg
//
