LOCUS       sequence-33310-e         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pTE-0X38-GCT1
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      533..542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgctagcca tatgtatatc tccttcgcat gcggatcaac ggcctggtcg acaagggcaa
       61 tacggtgatc gtcatcgaac ataacctgga cgtgatcggc cgtcaactcg ttgaggtacc
      121 ggtcggattc aaatggttcg tcgacggtct gatcccgcgg tgtgcaccga tgccctcacg
      181 gttccagcgg tcgaccaggt cctcgccgtg acgccccttg atcgcaaacg ccaccgaatg
      241 caccacggcc acagccaaag ccgacacgct ggttaccacg acgatgttca cggggggccc
      301 tcgcggatct ggcccaactg ctggacttcc accgaagcaa tcgagccgat gtcacgctgc
      361 aactggtgcg ggtgggcgac ccgcgggcat tcggctgcgt acccaccgac gaggaggacc
      421 gcgtagtcgc ctttctggag aagacggagc agacggtgct gttgggcgcc tgctggattc
      481 ccaggtcttc gaacaccgac acgctcgcgg cgtgatccaa tatttctaga tggccaccat
      541 ggtgacctca tgactcgcta gccagctggg gaccctagag gtccccttga aaaaattttt
      601 aaaataaaa
//
