LOCUS       sequence-333110-        4122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC16A6_STOP
FEATURES             Location/Qualifiers
     source          1..4122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1711..1810)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1827..1846)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1828..1846)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1851..1867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1851..1867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1969..1988)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1980..2789
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2047..2066)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2936..3524
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3425..3444
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3678..3695
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3854..3881)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3973..4059)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4108..4122,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4122,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacac attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccaccatg acccagaaca agctgaagct gtgctccaag gccaacgtgt
      181 acacagaggt gccagacgga ggatggggat gggcagtggc cgtgtctttc tttttcgtgg
      241 aggtgttcac ctatggcatc atcaagacat ttggcgtgtt tttcaacgac ctgatggatt
      301 ccttcaacga gtctaatagc aggatctctt ggatcatcag catctgcgtg ttcgtgctga
      361 ccttcagcgc ccctctggcc acagtgctgt ctaataggtt cggacacagg ctggtggtca
      421 tgctgggagg cctgctggtg tctaccggca tggtggccgc ctccttttct caggaggtga
      481 gccacatgta cgtggccatc ggcatcatct ccggcctggg ctattgcttt tctttcctgc
      541 caaccgtgac aatcctgagc cagtacttcg gcaagcggag aagcatcgtg accgcagtgg
      601 catccacagg agagtgtttt gccgtgtttg ccttcgcccc agcaatcatg gccctgaagg
      661 agcggatcgg ctggagatat tctctgctgt ttgtgggcct gctgcagctg aacatcgtga
      721 tcttcggcgc cctgctgcgg ccaatcttta tcagaggccc tgccagccca aagatcgtga
      781 tccaggagaa caggaaggag gcccagtaca tgctggagaa tgagaagacc cgcacatcta
      841 tcgacagcat cgattccggc gtggagctga ccacatcccc caagaacgtg cctacccaca
      901 caaatctgga gctggagcct aaggccgaca tgcagcaggt gctggtgaag acctccccaa
      961 gaccctctga gaagaaggcc ccactgctgg atttctctat cctgaaggag aagagcttta
     1021 tctgctacgc cctgttcggc ctgtttgcca ccctgggctt tttcgcccca agcctgtata
     1081 tcatccccct gggcatctcc ctgggaatcg accaggatag ggcagccttc ctgctgtcca
     1141 caatggccat cgcagaggtg ttcggaagaa tcggagcagg ctttgtgctg aatagggagc
     1201 ctatccgcaa gatctacatc gagctgatct gcgtgatcct gctgaccgtg agcctgtttg
     1261 ccttcacctt tgccacagag ttctggggcc tgatgagctg ttccatcttt ttcggcttta
     1321 tggtgggcac catcggcggc acacacatcc cactgctggc cgaggacgat gtggtgggca
     1381 tcgagaagat gagctccgcc gccggcgtgt atatcttcat ccagagcatc gcaggactgg
     1441 caggaccacc tctggcaggc ctgctggtgg accagagcaa gatctactcc agggcctttt
     1501 attcctgcgc agcaggaatg gcactggcag ccgtgtgcct ggccctggtg cgcccttgca
     1561 agatgggcct gtgccagcac caccactctg gagagaccaa ggtggtgagc cacaggggca
     1621 agacactgca ggacatcccc gaggatttcc tggagatgga tctggccaag aacgagcaca
     1681 gagtgcacgt gcagatggag cctgtgtaag acccagcttt cttgtacaaa gttggcatta
     1741 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1801 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     1861 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     1921 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     1981 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
     2041 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
     2101 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     2161 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     2221 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     2281 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     2341 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     2401 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     2461 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     2521 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     2581 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     2641 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     2701 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     2761 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     2821 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga
     2881 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2941 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3001 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3061 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3121 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3181 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3241 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3301 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3361 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3421 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3481 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3541 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3601 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3661 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3721 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3781 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     3841 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     3901 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     3961 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4021 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     4081 tcgcgttaac gctagcatgg atgttttccc agtcacgacg tt
//
