LOCUS       sequence-333111-        4167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC22A17_STOP
FEATURES             Location/Qualifiers
     source          1..4167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1756..1855)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1872..1891)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1873..1891)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1896..1912)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1896..1912)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2014..2033)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2025..2834
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2092..2111)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2981..3569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3470..3489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3723..3740
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3899..3926)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4018..4104)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4153..4167,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4167,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacac attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccaccatg gcctccgacc ctatcttcac cctggcacca cctctgcact
      181 gccactacgg agcctttcca cccaacgcct ctggctggga gcagcctcca aatgcctccg
      241 gcgtgtctgt ggcaagcgcc gccctggcag catccgccgc ctctagggtg gccacctcta
      301 cagacccaag ctgctccgga ttcgcaccac ctgacttcaa ccactgtctg aaggactggg
      361 attataacgg cctgcctgtg ctgaccacaa atgccatcgg ccagtgggac ctggtgtgcg
      421 atctgggctg gcaggtcatc ctggagcaga tcctgttcat cctgggcttt gccagcggct
      481 acctgttcct gggctatcct gccgatcgct ttggccggag aggcatcgtg ctgctgaccc
      541 tgggactggt gggaccatgc ggagtgggag gagcagcagc cggcagctcc acaggcgtga
      601 tggcactgcg gttcctgctg ggctttctgc tggcaggagt ggacctgggc gtgtacctga
      661 tgagactgga gctgtgcgat cctacccaga ggctgagggt ggccctggca ggagagctgg
      721 tgggcgtggg cggccacttc ctgtttctgg gactggccct ggtgtccaag gactggcggt
      781 tcctgcagag aatgatcaca gccccctgca tcctgttcct gttttatggc tggcctggcc
      841 tgtttctgga gtctgccagg tggctgatcg tgaagcgcca gatcgaggag gcacagagcg
      901 tgctgaggat cctggccgag cggaacagac cacacggaca gatgctggga gaggaggcac
      961 aggaggccct gcaggatctg gagaatacct gcccactgcc cgccacatct agcttcagct
     1021 ttgcctccct gctgaactac aggaatatct ggaagaacct gctgatcctg ggcttcacca
     1081 acttcatcgc ccacgccatc aggcactgtt accagccagt gggaggagga ggatctccaa
     1141 gcgacttcta tctgtgctct ctgctggcaa gcggaaccgc cgccctggca tgcgtgttcc
     1201 tgggcgtgac agtggatcgg tttggcaggc gcggcatcct gctgctgtcc atgaccctga
     1261 caggcatcgc ctctctggtg ctgctgggcc tgtgggactg tgagcacccc atctttccta
     1321 ccgtgtgggc acagcaggga aacccaaata gagatctgaa cgaggccgcc atcaccacat
     1381 tcagcgtgct gggcctgttt tcctctcagg cagcagcaat cctgtccaca ctgctggccg
     1441 ccgaagtgat cccaaccaca gtgaggggaa ggggactggg cctgatcatg gccctgggcg
     1501 ccctgggagg actgagcgga ccagcccaga ggctgcacat gggacacgga gccttcctgc
     1561 agcacgtggt gctggcagca tgcgcactgc tgtgcatcct gagcatcatg ctgctgcctg
     1621 agaccaagag gaagctgctg ccagaggtgc tgagggacgg agagctgtgc cggagaccat
     1681 ccctgctgag gcagccacca cctaccagat gtgatcacgt gcccctgctg gccacaccta
     1741 atccagccct gtaagaccca gctttcttgt acaaagttgg cattataaga aagcattgct
     1801 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     1861 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1921 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     1981 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     2041 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     2101 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     2161 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     2221 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     2281 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     2341 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     2401 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     2461 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     2521 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     2581 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     2641 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     2701 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     2761 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     2821 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     2881 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     2941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3121 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3601 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3661 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3721 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3781 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3841 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     3901 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     3961 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     4021 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     4081 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
     4141 catggatgtt ttcccagtca cgacgtt
//
