LOCUS       sequence-333113-        4245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC1A6_STOP
FEATURES             Location/Qualifiers
     source          1..4245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1834..1933)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1950..1969)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1951..1969)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1974..1990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1974..1990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2092..2111)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2103..2912
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2170..2189)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3059..3647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3548..3567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3801..3818
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3977..4004)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4096..4182)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4231..4245,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4245,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacac attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccaccatg agctcccacg gcaactccct gttcctgagg gagtctggac
      181 agcgcctggg aagggtggga tggctgcagc ggctgcagga gagcctgcag cagagagccc
      241 tgcggaccag actgaggctg cagaccatga cactggagca cgtgctgaga ttcctgcgga
      301 gaaatgcctt tatcctgctg accgtgtctg ccgtggtcat cggcgtgagc ctggcctttg
      361 ccctgaggcc ataccagctg acatatcgcc agatcaagta cttctccttt cccggcgagc
      421 tgctgatgag gatgctgcag atgctggtgc tgcctctgat cgtgtctagc ctggtgaccg
      481 gcatggcctc tctggacaac aaggccacag gcagaatggg catgagggcc gccgtgtact
      541 atatggtgac cacaatcatc gccgtgttca tcggcatcct gatggtgacc atcatccacc
      601 ctggcaaggg cagcaaggag ggcctgcaca gagagggcag gatcgagacc atcccaacag
      661 ccgacgcctt catggatctg atcaggaaca tgtttccccc taatctggtg gaggcctgct
      721 tcaagcagtt taagacccag tactccacac gcgtggtgac ccggacaatg gtgagaaccg
      781 agaatggatc tgagcctgga gcaagcatgc caccaccttt cagcgtggag aacggcacat
      841 cctttctgga gaatgtgacc cgcgccctgg gcacactgca ggagatgctg tccttcgagg
      901 agaccgtgcc agtgccagga tctgccaacg gcatcaatgc cctgggcctg gtggtgttct
      961 ctgtggcctt tggcctggtc atcggcggca tgaagcacaa gggccgcgtg ctgcgggact
     1021 tctttgatag cctgaacgag gccatcatgc ggctggtggg catcatcatc tggtatgccc
     1081 cagtgggcat cctgtttctg atcgccggca agatcctgga gatggaggat atggccgtgc
     1141 tgggaggaca gctgggaatg tacaccctga cagtgatcgt gggcctgttc ctgcacgccg
     1201 gcatcgtgct gcccctgatc tattttctgg tgacccacag aaatcctttc cccttcatcg
     1261 gaggaatgct gcaggccctg atcaccgcca tgggcacatc ctctagctcc gccaccctgc
     1321 ccatcacatt caggtgtctg gaggagggac tgggagtgga caggaggatc acacgctttg
     1381 tgctgcctgt gggcgccacc gtgaacatgg atggaacagc cctgtacgag gccctggcag
     1441 caatcttcat cgcccaggtg aacaattatg agctgaatct gggccagatc accacaatct
     1501 ccatcaccgc aacagcagca tctgtgggag cagcaggcat cccacaggcc ggcctggtga
     1561 ccatggtcat cgtgctgacc agcgtgggac tgccaaccga ggacatcaca ctgatcatcg
     1621 ccgtggactg gtttctggat cgcctgcgga ccatgacaaa cgtgctgggc gatagcatcg
     1681 gagcagccgt gatcgagcac ctgtcccaga gggagctgga gctgcaggag gcagagctga
     1741 ccctgccaag cctgggcaag ccttataagt ccctgatggc ccaggagaag ggagcatcta
     1801 gaggaagggg aggcaatgag agcgccatgt aagacccagc tttcttgtac aaagttggca
     1861 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     1921 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     1981 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2041 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     2101 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg
     2161 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     2221 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     2281 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     2341 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     2401 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     2461 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     2521 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     2581 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     2641 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     2701 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     2761 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     2821 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     2881 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact
     2941 ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg
     3001 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3181 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3721 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3781 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3841 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3901 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     3961 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4021 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4081 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4141 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     4201 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgtt
//
