LOCUS       sequence-333115-       18702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes rfb region of Shigella flexneri serotype 2a, which expresses
            Shigella O antigen in E. coli K-12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18702)
  AUTHORS   Macpherson DF, Morona R, Beger DW, Cheah KC, Manning PA
  TITLE     Genetic analysis of the rfb region of Shigella flexneri encoding the
            Y serotype O-antigen specificity.
  JOURNAL   Mol Microbiol. 1991 Jun;5(6):1491-9. doi: 
            10.1111/j.1365-2958.1991.tb00795.x.
  PUBMED    1724058
REFERENCE   2  (bases 1 to 18702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.1991.tb00795"; journalName: "Mol Microbiol"; 
            date: "1991-06"; volume: "5"; issue: "6"; pages: "1491-9"
COMMENT     Sequence Label: pPM2212
FEATURES             Location/Qualifiers
     source          1..18702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(558..1349,1350..1418))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1181..1200
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1419..1523)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1591..1609
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             5146..5157
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(14222..14241)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     terminator      15321..15415
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             17882..18073
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(18067..18089)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    18175..18315
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     18230..18249
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(18330..18347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(18501..18702,1..387))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18581..18600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
       61 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
      241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
      301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
      421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
      481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
      541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
      601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      661 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
      721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      841 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
      901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1141 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     1201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     1381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     1441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     1561 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     1621 caagaattct catgtttgac agcttatcat cgataacctc actgtcagcc acgctaatta
     1681 cgccatctta cgccagggat ttcataacca gattatcggt gccaacatta ccaactgtaa
     1741 gttcagcgac ttacagggcg acgccattga atggaacgtg gcaattaacg acagtgatat
     1801 tttgatatct gaccatgtca tcgagcgtat caactgtacc aacggcaaaa tcaactgggg
     1861 catcggcata ggccttgcgg gaagcactta tgataacaac tacccggaag accaggcagt
     1921 gaaaaacttt gtcgtggcga atatcacggg atcggattgt cggcagttga tccatgttga
     1981 aaatggtaaa cattttgtta ttcgtaatat caatgcccgc aatatcacgc cggatttcag
     2041 taagaaagcg ggcattgata acgcgacagt cgctatttac ggttgtgaca atttcgtgat
     2101 tgataatatt gaaatgatta atagcgccgg gatgttaatc ggctatgggg taattaaagg
     2161 caaatatctc tcgataccgc aaaatttccg agtgaataat attcaactgg ataataccca
     2221 tcttgcttat aaattgcgcg gcatccaaat ctccgccggg aatgccgtct cctttgtgtc
     2281 gctaactaac attgagatga agcgtgcctc gctggagtta cacaacaagc cgcaacatct
     2341 ttttatgcga aatatcaatg tgatgcagga atcctcagtt ggaccagcat tgagcatgaa
     2401 cttcgacatg cgcaaagacg ttcgcggcgt ctttatggcg aaaaaagaaa cactgctgtc
     2461 tcttgcaaat gttcatgcgg tgaatgaaag agggcaaagc tccgtcgata tcgacaggat
     2521 taatcaccat attgttaatg tggaaaagat taactttaga ttgccggaac ggagggagta
     2581 gatttgcgac cattcctgga aaaatggagc catacttagg aacaatgcta ctgcaatcca
     2641 caacgaagcg gcgtaacatc acaagtaatt cagtaatcaa ttcagggtaa ttgatgctgg
     2701 cgaaaaaaat cgaacaagct ataattcagc aaccatttta caggtggatg aaataatgac
     2761 gaatttaaaa gcagttattc ctgtagcggg tctcgggatg catatgttgc ctgccactaa
     2821 ggcgataccc aaagagatgc taccaatcgt cgacaagcca atgattcagt acattgttga
     2881 cgagattgtg gctgcaggga tcaaagaaat cctcctggta actcacgcgt ccaagaacgc
     2941 ggtcgaaaac cacttcgaca cctcttatga gttagaatca ctccttgagc agcgcgtgaa
     3001 gcgtcaactg ctggcggaag tacagtccat ctgtccgccg ggcgtgatca ttatgaacgt
     3061 gcgtcagggc gaacctttag gtttaggcca ctccattttg tgtgcgcgac ctgccattgg
     3121 tgacaaccca tttgtcgtgg tactgccaga cgttgtgatc gacgatgcca gcgccgaccc
     3181 gctacgttac aaccttgctg ccatgattgc acgtttcaac gaaacgggcc gcagccaggt
     3241 gctggcaaaa cgtatgccgg gtgacctctc tgaatactcc gtcatccaga ctaaagagcc
     3301 gctggaccgt gagggtaaag tcagccgcat tgttgaattt atcgaaaaac cggatcagcc
     3361 gcagacgctg gactcagaca tcatggccgt aggtcgctat gtgctttctg ccgatatttg
     3421 gccggaactg gaacgtactc agcctggtgc atggggacgt attcagctga ctgatgctat
     3481 tgccgagctg gcgaaaaaac aatccgttga tgcaatgctg atgaccggcg acagttacga
     3541 ctgcggcaaa aaaatgggct atatgcaggc gtttgtgaag tatggcctac gcaacctgaa
     3601 agaaggggcg aagttccgta aaggtattga gaagctgtta agcgaataat gaaaatctga
     3661 ccggatgtaa cggttgataa gaaaattata acggcagtga agattcgtgg cataagtttt
     3721 gctcattgaa ttttcctgcc gttgccgttt ttataaagcc tataaaaaca agaagttaca
     3781 tagctcatat tattcaaatt tttccaggat tttccttgtt tctacggctg tttggtaaga
     3841 caattaacgt ttgaattttt cggatttggc gcgagtggct aacgctcgcc acatcgttga
     3901 gatgcatgca gtgcactggt agctgttaag ccaggggcgg tagcgtgtat taatacctct
     3961 attaatcaaa tctggagcag tctatttcac agcatgctct atagctatat ggaataaaaa
     4021 agtgaagata cttgttactg gtggcgcagg gtttattggt tctgctgttg ttcgtcacat
     4081 aataaataat acgcaagata gtgttgttaa tgtcgataaa ttaacatacg ccgggaacct
     4141 ggaatcactc gctgatgttt ctgattctga acgttatgct tttgagcatg cagatatctg
     4201 cgatgctgta gccatgtcgc gtattttcgc acagcaccag ccagacgcgg tgatgcacct
     4261 ggcggcagag agccacgttg accgttcaat aacgggccct gcggcattta ttgaaaccaa
     4321 tattgtgggg acttatgttc ttttagaagc ggcgcgcaat tactggtctg ctctgaatga
     4381 tgaaaagaaa aaaagtttcc gctttcatca tatttctact gatgaagttt atggtgactt
     4441 accccatccg gatgaagcaa ataataacga agcgttaccg ctatttacgg aaacgacagc
     4501 ttacgcgcca agtagcccgt attctgcgtc taaagcttcc agcgatcatt tggtacgtgc
     4561 ctggaagcgg acttatggtt taccaaccat tgtgactaat tgctcgaaca attatggtcc
     4621 ttatcacttc ccggaaaaac ttattccatt ggttattctg aatgctctgg aaggtaaggc
     4681 attacctatt tatggtaaag gggatcaaat tcgcgactgg ttgtatgtag aggatcatgc
     4741 tcgtgcgtta tataccgtcg taaccgaagg taaagcgggt gaaacttata acattggtgg
     4801 acataacgaa aagaaaaaca tcgacgtagt gctcactatt tgtgatttgt tggatgagat
     4861 tgtaccgaaa gagaaatctt atcgtgagca aattacttat gttgccgatc gcccggggca
     4921 tgaccgccgt tacgccattg atgccgataa aattagccgc gaattgggct ggaaaccgca
     4981 agaaacgttt gagagcggga ttcgtaaaac ggtggaatgg tatctggcta atacaaattg
     5041 ggttgagaat gtgaaaagcg gtacttatca gtcgtggatt gaacaaaact atgagggccg
     5101 tcagtaatga atatcctgct tttcggcaaa acagggcagg tgggttggga actgcagcgt
     5161 gctctggctc ctctgggtaa cttgattgcg ctcgatgttc actccactga ttattgtgga
     5221 gatttcagca accccgaagg tgtggctgaa accgtcaaaa aaattcgccc cgatgttatt
     5281 gttaatgctg ctgctcacac tgcagtagac aaagcggaat cagaaccgaa tttcgcgcaa
     5341 ttacttaacg cgacatgcgt cgaagcgatt gcaaaagcag ctaatgaagt cggagcctgg
     5401 gttatacact actccactga ttatgttttc ccaggcaacg gtgacacgcc atggctggaa
     5461 acggatgcaa cagcaccgct aaatgtctac ggtgaaacca agctagctgg ggaaaaagcg
     5521 ttacaagaac attgcgcaaa gcatcttatt ttccgtacca gctgggtata cgctggtaaa
     5581 ggaaataact ttgccaaaac gatgttgcgc ctggcaaaag aacgcgaaga gttggctgta
     5641 ataaacgatc aattcggcgc accaacgggg gctgaattac tggctgattg caccgctcat
     5701 gccattcgcg tggccgcaaa taaaccagaa gttgctggtt tgtaccatct ggttgctggt
     5761 ggtacaacaa cctggcacga ttatgccgct ctggtatttg aagaagcgcg cagagcgggg
     5821 atcaaccttg ctcttaacaa acttaatgcc gtgccgacaa cggcctatcc cacaccagca
     5881 cgtcgtcccc ataattctcg cctcaatacc gaaaaatttc agcagaactt tgcgcttgtc
     5941 ttgcctgact ggcaggtggg cgtgaaacgt atgctcaacg aattatttac gaccacggca
     6001 atttaacaaa ttattgcatc tcgctcatga tgctagagcg agatgaatta aaaggaatgg
     6061 tgaaatgaaa acgcgtaaag gtattattct ggctggtggt tccggcactc gtctttatcc
     6121 tgtgacaatg gctgtgagca aacaactgct accgatttat gataaaccga tgatttatta
     6181 tccgctttca acgcttatgt tggcgggtat tcgagatatt ctgattatca gtacgccgca
     6241 ggatacacca cgttttcaac aattgcttgg tgatggaagc cagtgggggc ttaatctgca
     6301 atataaagta caaccgagtc cggatggtct ggcgcaagca tttatcattg gtgaagaatt
     6361 tataggtggt gatgattgtg cgctggtact tggtgataat atcttctatg gtcacgacct
     6421 gccgaaatta atggataccg ctgttaacag agagagtggc gcaacggttt ttgcctatca
     6481 cgtaaatgat cctgagcgct acggtgtggt ggaatttgat gataacggta cggcaattag
     6541 tctggaagaa aaaccgcagg aaccaaaaag taactatgcg gttactgggc tttatttcta
     6601 tgacaatgac gttgtagaaa tggcgaaaaa ccttaaacca tctgcacgtg gcgaactgga
     6661 aattaccgat attaaccgta tttatatgga ccaaggacgt ttgtctgtcg ctatgatggg
     6721 gcgtggttat gcatggctgg acacggggac acatcaaagt cttattgaag caagcaactt
     6781 cattgcaaca attgaagagc gtcaggggct gaaagtttcc tgcccggaag aaattgctca
     6841 ccgtaaaggc tttattgatg ctgagcaagt gaaggtatta gccgagccgc tgaagaaaaa
     6901 tgcttatggt cagtatctgc tgaaaatgat taaaggttat taataaaatg aacgtaatta
     6961 aaactgaaat tcctgatgtg ctaatttttg aaccaaaagt atttggtgat gagcggggct
     7021 tttttatgga aagcttcaat cagaaagttt tcgaagaggc tgtaggacga aaggttgagt
     7081 ttgttcagga taaccattct aaatcaacta agggtgtgtt acgcggactg cactatcagc
     7141 tggaacctta tgctcaagga aaactggtac gctgtgttgt cggtgaagtt tttgacgtag
     7201 ctgttgatat tcggaaatca tcaccaacat ttggcaagtg ggttggtgta aatttgtcgg
     7261 cggagaataa gcgtcaatta tggataccgg aaggattcgc tcatgggttt tgcgttctta
     7321 gtgatgaagc agaatttgtc tacaaaacaa ataatttcta ttcaaaaatg caagagcgtg
     7381 ggattttatg gagcgacaaa agtataaata tagaatggcc ggttcaaaat ccattgcttt
     7441 ctgataaaga tattaatggt caaaaatttg tagatgctga ttattttata tgataaagaa
     7501 atgtaataat gagcataata aaaaatagtg tctggaacct ttttggctat gcaataccaa
     7561 ctttaattgc tatcccctcg ctaggatttc tcgctcgagg tttggggcct gaaggtttcg
     7621 gtgtttatac aattgcaatt gcacttgttg ggtatgctgg aatttttgat gtaggcctga
     7681 ctcgctctgt tattagagaa attgcaattc atcgtgataa tcatcatgaa agaaccaagg
     7741 taatttcaac aagtacatct tttttggtgc tattttcatg ctttggtgct tttttattat
     7801 tgattttctc tgatggaatt gttaattatc taaaaatttc tggtgttgaa catagtgata
     7861 tacaactagc atttaaactg ttggctattt gcattccatt atttattcta aatcaattat
     7921 ggtcagccat tcttgagggg gatgaaaaat ttggcattgt aaatattcaa aaatctatat
     7981 caagctcttg catcgcggga attccggcca tatttgtttt ttatagtgct acattgtcgg
     8041 cagcggttgc tggtttaata tttgcaagag ttatttcgat tttagtctct gcctattatg
     8101 tcaggaatga tattaaaatt tcgggggttc atttttgtta taaaaccttt aaacgactct
     8161 ttttctttgg cggttggatg acagtaagta atatcataag tccggtcatg gtgtattttg
     8221 atcgatttat agtgtcaaat attatggggg cagataaagt tgcattttat tctgcaccag
     8281 cggaggttat tctaaaatta ggaataatac ctgcagcaat cgggagggca gtgtttccaa
     8341 ggttaagtaa catcaaagac tttaaagaat ttaaacgtaa tgtaaataaa tcattgcttt
     8401 taatgtttct aatctgtttg ccggtgataa tcataggctt gttatattca gggcttgtat
     8461 tgaaaatatg gtttggtgag aattatcaaa ttaattcctt taatatatta aatgtgttat
     8521 tgatcggttt tttcttcaat gcgctggcaa tgataccatt ctctgcaatc caggcattag
     8581 gaaaatctaa aattactgct ttgattcatt gtgctgaatt ggttccttat ttagcccttt
     8641 tgtattttat ggtcgaaaaa tatgggttac tgggtgccgc aatatcctgg agcatacgtg
     8701 taattttaga tgctctgtta ttacaatggc tttatactag aatgtgttct gtatatgaaa
     8761 actaaaatta accaattact gagtcacttg gataatgaat agtaatattt acgctgtcat
     8821 tgtgacatat aatcccgaac ttaaaaatct gaatgcactg atcactgaac taaaggagca
     8881 aaattgttac gttgtagtag tcgataatag gactaatttc accttgaaag ataaattagc
     8941 agacattgaa aaagttcatt taatttgtct tggtaggaat gaaggtatag caaaagcaca
     9001 aaatattggg attcgatatt ctcttgaaaa aggggcagag aagataattt tttttgatca
     9061 agatagccgt attcgcaatg agtttatcaa aaaactttcc tgctatatgg ataatgaaaa
     9121 tgcaaaaata gctggcccag tttttattga tagagataag tcacattatt atcctatttg
     9181 taatatcaaa aaaaatggtc ttcgagagaa aattcatgtc actgaaggac agacaccgtt
     9241 taaaagttca gtaacaatct catccggaac catggtttca aaagaagttt ttgagattgt
     9301 tggaatgatg gatgaggaac tttttattga ttatgtcgat acagaatggt gtcttagatg
     9361 cttaaactat ggcatattag ttcatatcat tcctgatata gaaatggttc atgctattgg
     9421 ggataagtca gtaaaaatct gtgggattaa cataccaatt cactcgccag tacgtcgtta
     9481 ttatcgagta agaaatgcat ttcttttgct tagaaaaaat catgtgcctc ttttactctc
     9541 tattagggaa gttgtttttt ctttaattca tacgacttta attatcgcaa ctcaaaaaaa
     9601 taaaattgaa tatatgaaaa aacatatttt ggctacgctg gatggaataa gaggaattac
     9661 tgggggcggt cgttataatg catagttctg atcaaaaaag agtagctgta cttatggcta
     9721 cgtataatgg cgaatgctgg attgaggaac aattaaaaag tattattgaa caaaaagatg
     9781 tagacatctc aatttttata tcagatgatt tatcaactga taatacatta aatatttgtg
     9841 aagaatttca attatcatat ccctcaatta taaatatact tccaagcgtc aataagtttg
     9901 ggggggcagg taaaaatttt tatagattaa ttaaagatgt cgatcttgaa aactatgatt
     9961 atatctgttt tagtgatcag gacgatattt ggtataaaga taaaataaaa aatgcaattg
    10021 actgcttggt atttaataat gcgaattgtt attccagcaa tgtaattgca tattatccat
    10081 cagggcgaaa aaatttagtt gataaggctc agtcgcaaac tcaattcgat tatttttttg
    10141 aagcagcagg tccggggtgt acatatgtta taaaaaaaga aacgcttatt gaatttaaga
    10201 agttcatcat aaataataaa aatgctgcac aagatatctg tctgcatgat tggtttttat
    10261 attcatttgc gcggacgaga aattattcgt ggtatattga tagaaaacca acaatgctgt
    10321 acagacagca tgaaaacaac caagtgggag caaacattag ttttaaagca aaatacaaac
    10381 ggcttggttt ggtgagaaac aagtggtatc gaaaagaagt aactaaaata gctaatgcct
    10441 tagctgatga tagctttgtg aataatcaat tgggtaaagg ttatataggc aatttgatct
    10501 tggctttaag tttttggaag ttaaggcgga aaaaggctga taaaatttat attttattaa
    10561 tgttgatttt aaacatattt taaaaaacat caccttacga atatgaataa tataaataaa
    10621 atttttataa catttttatg tattgaactg attattggtg gtggtggaag attactggag
    10681 ccattgggaa tattcccttt gcgatattta ttatttgtat ttagttttat acttttaatt
    10741 tttaatttag ttacattcaa tttttcaatc acccaaaaat gtgtcagtct ttttatatgg
    10801 ttgcttttat ttccttttta tggcttcttt gtcggcttat tagctggtaa taaaataaat
    10861 gatatactgt ttgatgtgca accatacctt tttatgctgt cacttatata tctatttaca
    10921 ctaagatata ctttaaaagt attttcatgt gagattttta ttaaaatagt taatgcattt
    10981 gcattatatg gatcactgtt atatatttca tacataattt tgttgaattt cggtttgtta
    11041 aattttaatt taatttatga acacttatca ttgactagcg agttcttttt tcgtcccgat
    11101 ggggcttttt tttccaaatc cttctacttt tttggtgtcg gtgcgattat cagttttgtc
    11161 gacaaaaaat atttaaaatg tctcataata gtgcttgcga tattattgac agaatcaaga
    11221 ggtgtattac tttttacaac attatcactg ttattagcca gttttaaatt acataagcta
    11281 tatttaaata ctattataat aatattgggc agcgttctat ttataattat gctttacatg
    11341 gtcggatcac gcagtgaaga ttctgactct gttagattta atgatttata tttttattat
    11401 aaaaatgttg atttagcgac gttcttgttt ggaagaggat ttggttcatt tatattagat
    11461 cgattaagga ttgaaatagt acctcttgag atacttcaga aaacaggcgt tattggtgta
    11521 tttatatcat tagttcctat gttgcttatc tttttgaaag gctatttttt aaatagtaca
    11581 aaaacatcat taatgatgtc gttaatactt tttttcagta ttaccgtttc tataactaat
    11641 ccatttcttt ttacacccat gggaattttt attataggcg ttgtagtttt atgggtattt
    11701 tctatagaaa atatccaaat tagtaataac ctcacttctg gagcaaaata aaggatgtta
    11761 aaaataggga agttattgac cagttctttt tttagttatt ttttaattgg aattgtcaat
    11821 actgctttac attggggggt attttatgcg tgttataata atttagcttt tggtcaaggc
    11881 cgaagtaaca ttgttggttt tatatgtgca gcaacctttt ctttttttgc aaatgcaaga
    11941 tgctcattta aagtatctgc aaccaaggct agatatttta tatttatatt ttttatgggg
    12001 gcgatgagtt atttattcgg cgttttattt gatctcttag ctttatctcc aatatttaca
    12061 ttgttcacct tttctctttt tagtttggtt ttaggatatt gcgcatcaaa atatttcatt
    12121 tttcgttagt aaacgatttg ttaaaaacac aattacttgc acattagcta aatagtttat
    12181 atgaggggct tatgtatcac tcttttaata tcgctgtaat tattccttgt tataatgagc
    12241 agaaggcaat agcaaaagtc ataaatgatt tcaaaacgaa tattccaact gcttctatat
    12301 atgtatttga taataattcg actgacagta cagcacaagt ggcagaggat gctggagcaa
    12361 cagttcactc tgttcctctg aaaggcaagg ggaatgtcgt aagacgtatg ttctcagatg
    12421 tagatgcgga tatttatcta atggttgatg gagatgacac ttatgatgca agttctgctc
    12481 cagaaatgat taaccatctc ataaaaaatc aattggatat ggttgtagga tgtcgtcaag
    12541 agaatggaga tcaaaataca tacagaaaag ggcatcgctg aggaaataaa ttgttgactg
    12601 gtactgttca gactatcttt aaagggaatt ttactgatat gttgtcgggg tatcgtgtat
    12661 tttccagacg ctatgttaaa tcttttccat gtctatcaag gggatttgaa atcgaaactg
    12721 agcttacaat tcatgcttta gaattacgta tgaagtatgg tgaggttaat acaaaatatg
    12781 gcgagcgatc tgaagggagt gtgagcaaac tatcaacttg gagtgatggg ttcaagattc
    12841 tgaaaacaat aattaaactt tactcgcttg aacggccact atactttttt agtattatcg
    12901 gcgttttact tgctgctttg tctataattt taggattgcc catcattgtt gattatattg
    12961 atacaggttt ggtgagaaga tttccgactg cctttttaac tgcctccatc atgttatcgt
    13021 caataatggc atttgtatgt ggcattattt tgcattcaaa tacaacaact agacgtgaaa
    13081 tgaaggcatt attttatctt tctgaaaaaa actataaact gataatgtaa atagagtatt
    13141 ttactatgat tgaattgccg ttgtatttgt atgttgtgta ttgttttgct tttggaatga
    13201 cattttttgt cattggtaat attgtattgt catctttttt taaggagggg gtggcaggac
    13261 ttaacaaatt ttctttattt tttattgcct atgcataggg atgtctttaa atattttgat
    13321 aatttacttt ctgggcatgg tcgggcaatt taataagatt gctatctttt taatttttac
    13381 ggtgtgttgg gttttaagca ttattaaacg tcagcaattt agatggctgg caataaataa
    13441 tattgagttc agtacgctgt ttgttatttt atttcttgtt ttgatttttg tggttacatt
    13501 gttatcgtcc ctgcgagctc ccggtgattg ggatgatact atgtatcact tacctttggc
    13561 tcgttcatta gttgagcacc atgcaattgt tgttgaacaa tatttgcgtt ttcctctgtt
    13621 cccgcaaaat gcagatcttt taatggcttt aggtttacag ttaggagatg taaggttagc
    13681 tcagttttta gcaaatatct gtttttttgt aatagcctgt ggacttgttg gctgttcatg
    13741 ggaaattacg aaaacttatt atccaggcat tattgctaca atcctactat ttactatcaa
    13801 tccacttaaa gatcatcttg gttatgcata tattgattta acgctatctt tgttttgctg
    13861 cagccagtat tcttatatat attcgctcag aaaacaataa aacacaaaat gaattaattt
    13921 ttttatgtgg tttttttatt ggaattgctt gtggcgtaaa actatttggt gccatatatg
    13981 gagccatcat atttgttata ttgatagcga aaaagagaaa catacagaca tttcttattt
    14041 ttagcttggc aacaattctt ggtggcattt attggtatgt ccgtagtttt tggatctctg
    14101 gtgatccttt tagtcctgct ggaggaaata tatttggtca ctacctatgg aatgaaattg
    14161 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
    14221 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
    14281 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
    14341 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
    14401 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc
    14461 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
    14521 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
    14581 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
    14641 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag
    14701 caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg
    14761 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
    14821 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
    14881 ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg
    14941 atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta
    15001 taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga
    15061 atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
    15121 tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg
    15181 ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc
    15241 gcatgatctg ccacattacg ctcctgtccg gcaaagttac ctctgccgaa gttgagtatt
    15301 tttgctgtat ttgtcataat gactcctgtt gatagatcca gtaatgacct cagaactcca
    15361 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatcgcag
    15421 caacttgtcg cgccaatcga gccatgtcgt cgtcaacgac cccccattca agaacagcaa
    15481 gcagcattga gaactttgga atccagtccc tcttccacct gctgatctgc gacttatcaa
    15541 cgcccacagc ttccgctgtc ttctcagttc caagcattgc gattttgtta agcaacgcac
    15601 tctcgattcg tagagcctcg ttgcgtttgt ttgcacgaac catatgtaag tatttcctta
    15661 gataacaatt gattgaatgt atgcaaataa atgcatacac cataggtgtg gtttaatttg
    15721 atgccctttt tcagggctgg aatgtgtaag agcggggtta tttatgctgt tgtttttttg
    15781 ttactcggga agggctttac ctcttccgca taaacgcttc catcagcgtt tatagttaaa
    15841 aaaatctttc ggcctgcatg aatggccttg ttgatcgcgc tttgatatac gccgagatct
    15901 tggcagtcac agcatgcgca tatccatgct tcgaccatgc gctcacaaag taggtgaatg
    15961 cgcaatgtag tacccacatc gtcatcgctt tccactgctc tcgcgaataa agatggaaaa
    16021 tcaatctcat ggtaatagtc catgaaaatc cttgtattca taaatcctcc aggtagctat
    16081 atgcaaattg aaacaaaaga gatggtgatc tttctaagag atgatggaat ctcccttcag
    16141 tatcccgatg gtcaatgcgc tggatatggg atagatggga atatgctgat ttttatggga
    16201 cagagttgcg aactgttccc aactaaaatc attttgcacg atcagcgcac tacgaacttt
    16261 acccacaaat agtcaggtaa tgaatcctga tataaagaca ggttgataaa tcagtcttct
    16321 acgcgcatcg cacgcgcaca ccgtagaaag tctttcagtt gtgagcctgg gcaaaccgtt
    16381 aactttcggc ggctttgctg tgcgacaggc tcacgtctaa aaggaaataa atcatgggtc
    16441 ataaaattat cacgttgtcc ggcgcggcga cggatgttct gtatgcgctg tttttccgtg
    16501 gcgcgttgct gtctggtgat ctgccttcta aatctggcac agccgaattg cgcgagcttg
    16561 gttttgctga aaccagacac acagcaactg aataccagaa agaaaatcac tttacctttc
    16621 tgacatcaga agggcagaaa tttgccgttg aacacctggt caatacgcgt tttggtgagc
    16681 agcaatattg cgcttcgatg acgcttggcg ttgagattga tacctctgct gcacaaaagg
    16741 caatcgacga gctggaccag cgcattcgtg acaccgtctc cttcgaactt attcgcaatg
    16801 gagtgtcatt catcaaggac gccgctatcg caaatggtgc tatccacgca gcggcaatcg
    16861 aaacacctca gccggtgacc aatatctaca acatcagcct tggtatccag cgtgatgagc
    16921 cagcgcagaa caaggtaacc gtcagtgccg ataagttcaa agttaaacct ggtgttgata
    16981 ccaacattga aacgttgatc gaaaacgcgc tgaaaaacgc tgctgaatgt gcggcgctgg
    17041 atgtcacaaa gcaaatggca gcagacaaga aagcgatgga tgaactggct tcctatgtcc
    17101 gcacggccat catgatggaa tgtttccccg gtggtgttat ctggcagcag tgccgtcgat
    17161 agtatgcaat tgataattat tatcatttgc gggtcctttc cggcgatccg ccttgttacg
    17221 gggcggcgac ctcgcgggtt ttcgctattt atgaaaattt tccggtttaa ggcgtttccg
    17281 ttcttcttcg tcataactta atgtttttat ttaaaatacc ctctgaaaag aaaggaaacg
    17341 acaggtgctg aaagcgagct ttttggcctc tgtcgtttcc tttctctgtt tttgtccgtg
    17401 gaatgaacaa tggaagtcaa caaaaagcag ctggctgaca ttttcggtgc gagtatccgt
    17461 accattcaga actggcagga acagggaatg cccgttctgc gaggcggtgg caagggtaat
    17521 gaggtgcttt atgactctgc cgccgtcata aaatggtatg ccgaaaggga tgctgaaatt
    17581 gagaacgaaa agctgcgccg ggaggttgaa gaactgcggc aggccagcga ggcagatctg
    17641 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg
    17701 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc
    17761 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
    17821 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc
    17881 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
    17941 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
    18001 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
    18061 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    18121 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
    18181 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
    18241 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    18301 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    18361 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    18421 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    18481 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    18541 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    18601 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    18661 gcctttctcc cttcgggaag cgtggcgctt tctcatagct ca
//
