LOCUS       sequence-33312-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMEH-P38-mRFP
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    133..157
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(649..673)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(677..697)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(717..733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(717..733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(730..752)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
       61 agcgcatgaa ctccttgatg atggccatgt tgtcctcctc gcccttgctc accattatta
      121 acctcctgca tgcccacttt gtacaagaaa gctgggtcgg atcaacggcc tggtcgacaa
      181 gggcaatacg gtgatcgtca tcgaacataa cctggacgtg atcggccgtc aactcgttga
      241 ggtaccggtc ggattcaaat ggttcgtcga cggtctgatc ccgcggtgtg caccgatgcc
      301 ctcacggttc cagcggtcga ccaggtcctc gccgtgacgc cccttgatcg caaacgccac
      361 cgaatgcacc acggccacag ccaaagccga cacgctggtt accacgacga tgttcacggg
      421 gggccctcgc ggatctggcc caactgctgg acttccaccg aagcaatcga gccgatgtca
      481 cgctgcaact ggtgcgggtg ggcgacccgc gggcattcgg ctgcgtaccc accgacgagg
      541 aggaccgcgt agtcgccttt ctggagaaga cggagcagac ggtgctgttg ggcgcctgct
      601 ggattcccag gtcttcgaac accgacacgc tcgcggcgtg atccaataag cctgcttttt
      661 tgtacaaact tgaattcaac ttttctatac aaagttgata gcttggcgta atcatggtca
      721 tagctgtttc ctgtgtgaaa ttgttatccg ctggtatcag ctcactcaaa ggcggtaata
      781 cggttatcca cagaatca
//
