LOCUS       sequence-33313-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMEH-P38-mRFP
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(283..303)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(311..328)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(311..327)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(320..342)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atgtacccgg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
       61 ggcggccact acgacgcgga ggtcaagacc acctacaagg ccaagaagcc ggtgcagctg
      121 ccgggcgcct acaacgtcaa catcaagttg gacatcacct cgcacaacga ggactacacc
      181 atcgtggaac agtacgaacg cgccgagggc cgccactcga ccggcggtat ggacgagctg
      241 tacaagtagt gacccgggct gcaggaattc gatatcaagc ttcaacttta ttatacatag
      301 ttgataattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      361 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      421 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
      481 attttctcct tacgcatctg tgcggtattt cacaccgcat atggggattg gtggcgacga
      541 ctcctggagc ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca
      601 gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt
      661 aaggaaatcc attatgtact atttaaaaaa cacaaacttt tggatgttcg gtttattctt
      721 tttcttttac ttttttatca tgggagccta cttcccgttt ttcccgattt ggctacatga
      781 catcaaccat atcagcaaaa gtgatacggg tattattttt gccgctatt
//
