LOCUS       sequence-333133-        8888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a negative control sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Bieging-Rolett KT, Kaiser AM, Morgens DW, Boutelle AM, Seoane JA, 
            Van Nostrand EL, Zhu C, Houlihan SL, Mello SS, Yee BA, McClendon J, 
            Pierce SE, Winters IP, Wang M, Connolly AJ, Lowe SW, Curtis C, Yeo 
            GW, Winslow MM, Bassik MC, Attardi LD
  TITLE     Zmat3 Is a Key Splicing Regulator in the p53 Tumor Suppression 
            Program.
  JOURNAL   Mol Cell. 2020 Nov 5;80(3):452-469.e9. doi: 
            10.1016/j.molcel.2020.10.022.
  PUBMED    33157015
REFERENCE   2  (bases 1 to 8888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.10"; journalName: "Mol Cell"; date: 
            "2020-11-5- 5"; volume: "80"; issue: "3"; pages: "452-469.e9"
COMMENT     Sequence Label: pMCB320-sgNC.ACOCA
FEATURES             Location/Qualifiers
     source          1..8888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             750..930
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    977..1102
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1599..1832
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2017..2061
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2359..2476
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2729..2762)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2766..2786
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3030..3241
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3242..4180
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4137..4157
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4201..4797
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4201..4220)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4697..4717
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4807..4860
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4867..4876
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4873..5583
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5018..5036)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5286..5305
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(5603..5636)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5692..6280
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5745..5765)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6163..6174)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6283..6299)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6284..6300)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6809..6989
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7051..7639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7131..7150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7810..8601,8602..8670))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8433..8452
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8671..8775)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8850..8869)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
       61 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
      121 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      781 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      841 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      901 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      961 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     1021 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     1081 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     1141 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1201 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1261 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1321 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1381 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1441 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1501 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1561 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1621 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1681 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1741 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1801 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1861 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1921 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1981 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     2041 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     2101 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     2161 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2221 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2281 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     2341 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2401 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2461 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc
     2521 gggcccgctc tagagatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacgga
     2581 cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag
     2641 taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct
     2701 acattttaca tgataggctt ggatttctat aacttcgtat agcatacatt atacgaagtt
     2761 ataaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat
     2821 aagtatccct tggagaacca ccttgttggg caaactatct ccaactggtt taagagctaa
     2881 gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc
     2941 accgagtcgg tgcttttttt ctcgagtact aggatccatt aggcggccgc gtggataacc
     3001 gtattaccgc catgcatgtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     3061 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     3121 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     3181 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     3241 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     3301 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     3361 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     3421 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     3481 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     3541 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     3601 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     3661 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     3721 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     3781 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     3841 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     3901 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     3961 gcacctcgat tagttctcgc gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     4021 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     4081 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     4141 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag ctagcccacc
     4201 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     4261 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     4321 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4381 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4441 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     4501 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     4561 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     4621 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     4681 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     4741 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccgga
     4801 tcgggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca
     4861 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     4921 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     4981 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     5041 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     5101 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     5161 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5221 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     5281 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     5341 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     5401 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     5461 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     5521 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     5581 taagaattcg tcgagggacc taataacttc gtatagcata cattatacga agttatacat
     5641 gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct
     5701 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5761 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5821 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5881 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5941 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6001 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6061 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6121 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6181 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6241 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     6301 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     6361 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     6421 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6481 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     6541 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     6601 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     6661 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     6721 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     6781 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     6841 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6901 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6961 cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag
     7021 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7081 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7141 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7201 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7261 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7321 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7381 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7441 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7501 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7561 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7621 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7681 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7741 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7801 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7861 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7921 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7981 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8041 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8101 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8161 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8221 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8281 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8341 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8401 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8461 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8521 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8581 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8641 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8701 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8761 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     8821 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8881 cagtatct
//
