LOCUS       sequence-33314-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMEH-P38-che-ssrAec3
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    121..145
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(637..661)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(665..685)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(705..721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(705..721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(718..740)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag cgcatgaact
       61 ccttgatgat ggccatgttg tcctcctcgc ccttgctcac cattattaac ctcctgcatg
      121 cccactttgt acaagaaagc tgggtcggat caacggcctg gtcgacaagg gcaatacggt
      181 gatcgtcatc gaacataacc tggacgtgat cggccgtcaa ctcgttgagg taccggtcgg
      241 attcaaatgg ttcgtcgacg gtctgatccc gcggtgtgca ccgatgccct cacggttcca
      301 gcggtcgacc aggtcctcgc cgtgacgccc cttgatcgca aacgccaccg aatgcaccac
      361 ggccacagcc aaagccgaca cgctggttac cacgacgatg ttcacggggg gccctcgcgg
      421 atctggccca actgctggac ttccaccgaa gcaatcgagc cgatgtcacg ctgcaactgg
      481 tgcgggtggg cgacccgcgg gcattcggct gcgtacccac cgacgaggag gaccgcgtag
      541 tcgcctttct ggngaagacg gagcagacgg tgctgttggg cgcctgctgg attcccaggt
      601 cttcgaacac cgacacgctc gcggcgtgat ccaataagcc tgcttttttg tacaaacttg
      661 aattcaactt ttctatacaa agttgatagc ttggcgtaat catggtcata gctgtttcct
      721 gtgtgaaatt gttatccgct ggtatcagct cactcaaagg cggtaatacg gttatccaca
      781 gaatcagggg ataacgcagg aaagaacatg gtgacctcat gactcgctag c
//
