LOCUS       sequence-333143-        5952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV transfer plasmid for neuronal expression of jRex-GECO..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Grodem S, Nymoen I, Vatne GH, Rogge FS, Bjornsdottir V, Lensjo KK, 
            Fyhn M
  TITLE     An updated suite of viral vectors for in vivo calcium imaging using 
            intracerebral and retro-orbital injections in male mice.
  JOURNAL   Nat Commun. 2023 Feb 4;14(1):608. doi: 10.1038/s41467-023-36324-3.
  PUBMED    36739289
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-4"; volume: "14(1):608. doi"; pages: " 
            10.1038/s41467-023-36324-3"
COMMENT     Sequence Label: AAV-hSyn-jRex-GECO
FEATURES             Location/Qualifiers
     source          1..5952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     163..182
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   324..453
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        664..1111
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      1124..1133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1145..1201
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     1897..1916
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2408..2996
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2461..2481)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2879..2890)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3016..3492
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3215..3234)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3538..3678
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3538..3667
                     /label=AAV2 ITR
     rep_origin      3753..4208
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3840..3859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4050..4071
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4225..4244)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4344..4366
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4404..4422)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4490..4594
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4595..4663,4664..5455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4813..4832)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5626..5952,1..262)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
       61 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      121 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      181 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      241 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      301 ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc
      361 cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag
      421 agggagtggc caactccatc actaggggtt cctgcggccg cacgcgttgc aaagatggat
      481 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
      541 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
      601 gttgggggga ggggtcggca gcaaatggtt aattaatcta gactgcagag ggccctgcgt
      661 atgagtgcaa gtgggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac
      721 cgaccccgac ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc
      781 agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc
      841 gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg
      901 cgcgctgacg tcactcgccg gtcccccgca aactcccctt cccggccacc ttggtcgcgt
      961 ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg
     1021 gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag
     1081 cggaggagtc gtgtcgtgcc tgagagcgca gtcgagagga tccgccacca tggtcgactc
     1141 ttcacgtcgt aagtggaata aggcaggtca cgcagtcaga gctataggtc ggctgagctc
     1201 acgttgggtt tccgagtgga tgtaccccga ggacggcgcc ctgaagagcg tgatcaagga
     1261 ggggttgagg ctgaaggacg gcggccacta cgccgccgag gtcaggacca cctacaaggc
     1321 caaaaagccc gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc
     1381 ccacaacgag gactacacca tcgtggaaca gtgcgaacgc gccgagggcc gccaccccac
     1441 cggcggcatg gtcgggctgt acaagggagg tacaggcggg agtctggtga gcaagggcga
     1501 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     1561 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt
     1621 tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     1681 gtcccttcag ttcatgtacg gctccaaggc ctacattaag cacccagccg acatccccga
     1741 ctacttcaag ctgtccttcc ccgagggctt caggtgggag cgcgtgatga tcttcgagga
     1801 cggcggcatt attcacgtta accaggactc ctccctgcag gacggcgtat tcatctacaa
     1861 ggtgaagctg cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat
     1921 gggctgggag cctacgcgtg acgatctgac tgaagagcag atcgcagagt ttaaagaggc
     1981 tttctcccta tttgacaagg acggggatgg gacgataaca accaaggagc tggggacggt
     2041 gttccggtct ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt
     2101 agatgccgac ggtgacggca cattcgactt ccctgagttc ctgacgatga tggcaaggaa
     2161 aatgaatgac tcagacagtg aagaggaaat tagagaagcg ttccgcgtgt ttgataagga
     2221 cggcaatggc tacatcggcg cagcagagct tcgccacgtg atgacagacc ttggagagaa
     2281 gttaacagat gaggaggttg atgaaatgat cagggtagca gacatcgatg gggatggtca
     2341 ggtaaactac gaagagtttg tacaaatgat gacagcgaag taggaattcc gtacaaagct
     2401 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2461 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2521 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2581 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2641 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2701 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2761 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2821 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2881 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2941 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3001 ataccggctc gagacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     3061 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     3121 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     3181 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     3241 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     3301 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     3361 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     3421 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     3481 ttccctgtcc ttctcgagct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
     3541 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3601 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3661 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3721 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     3781 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     3841 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3901 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3961 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4021 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4081 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4141 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4201 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4261 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4321 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4381 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4441 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4501 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4561 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4621 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4681 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4741 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4801 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4861 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4921 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4981 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5041 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5101 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5161 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5221 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5281 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5341 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5401 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5461 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5521 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5581 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5641 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5701 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5761 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5821 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5881 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5941 ctgaacgggg gg
//
